Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression.

Abstract: Objective To investigate the expression of aquaporin-1 (AQP1) in the human peritoneum and to evaluate the effect of peritoneal dialysis (PD) on its expression. Methods Peritoneal biopsies were obtained from normal subjects (n = 10), uremic nondialysis patients (n = 12) at catheter insertion and PD patients (n = 10) at the time of catheter removal, reinsertion or renal transplantation. Western blot, immuno-histochemical staining and reverse transcript-polymerase chain reaction (RT-PCR) techniques were used to investigate AQP1 expression. Results All peritoneal samples expressed AQP1 at both mRNA and protein levels. Western blot revealed a major band at 28 kD as well as more diffuse bands between 35 and 50 kD. The 28 kD band represents the nonglycosylated form of the protein while the 35 - 50 kD bands correspond to glycosylated AQP1. Immunohistochemical staining found the positive deposits were distributed in the mesothelial cells, endothelial cells of capillaries, venules and small veins, whereas no signal was detected in the arterioles. Semi-quantitative analysis showed that AQP1 expression was remarkably stable in all samples, whatever their origin (P > 0.05). Conclusions Our findings suggested that AQP1 is the molecular counterpart of an ultra small pore during PD. Secondly, the peritoneal mesothelial cell might also be involved in peritoneal transcellular water transport. As regards whether or not the structural or distributional alterations of AQP1 in the peritoneum may be more obviously expressed during PD, further study is needed.

Microanatomy of endoscope-assisted glabellar nasal keyhole approach.

Abstract: Objective: To make a study of the detailed microanatomy of endoscope-assisted glabellar nasal keyhole approach (GNKA) to be used clinically. Methods: 10 dry adult Chinese skulls were measured and 10 perfused cadaveric heads were dissected to mimic the GNKA. Results: Average distances between bilateral supraorbital foramen/incisure, supratrochlear incisure, medial orbital wall were 47.71 ± 3.61 mm, 33.67 ± 3.82 mm and 24.34 ± 1.29 mm, respectively, in dry skulls. Average distance from nasion to medial intercanthus line (ICL) was 10.31 ± 1.02 mm in cadaveric heads. The nasion was 3.95 ± 0.45 mm above the cribriform plate (CP) and the ICL is 6.25 ± 0.71 mm below. With the assistance of endoscopes, GNKA can expose all extradural structures near the midline from the anterior skull base to the ventral brain stem corresponding to the clivus. The exposed clival dura was 19.18 ± 1.44 mm in width and 25.44 ± 1.25 mm in length. Intradural structures such as frontal base, parasellar area, longitudinal fissure and the third ventricle floor, can also be exposed. Conclusion: GNKA is a minimally invasive and effective anterior skull base approach. Determining the inferior margin of incision by ICL instead of nasion is more convenient and can expose the subcranial area better.

Hand-foot syndrome (HFS) and asthenia/fatigue (A/F) as biomarkers of sunitinib efficacy in Chinese patients (pts) with metastatic renal cell carcinoma (mRCC).

Abstract: e15622 Background: In an open-label phase IV study of sunitinib as 1st-line treatment (Tx) in Chinese pts with mRCC, median progression-free survival (PFS) and overall survival (OS) were 61.7 and 133.4 wk, respectively; objective response rate (ORR) was 31.1% (Ann Oncol 2012;23:851P). We retrospectively investigated correlations between on-Tx HFS or A/F and efficacy endpoints in pts from this trial. Methods: AEs were recorded and graded using NCI CTCAE v3. Median PFS and OS were estimated by Kaplan−Meier method. The log-rank test was used to compare PFS and OS between groups with and without HFS or A/F. Fisher’s exact test was used for ORR. Multivariate and time-dependent covariate analyses were conducted to assess the influence of various prognostic factors on the association between AEs and efficacy and to address potential bias from longer drug exposure, respectively. Landmark analyses were used to compare outcomes in pts with and without HFS or A/F after 6 and 12 wk of Tx. Results: Of 103 pts included...

Effect of Sophora flavescens Ait on cultured beating myocardial cells of coxsackie B3 virus infected newborn rat

Abstract: OBJECTIVE Coxsackie B viruses (CVB3) are considered to be the most common etiologic agents of viral myocarditis. There are not any special anti-CVB3 drugs yet. From previous studies, the anti-CVB3 affect of sophora flavescens ait (SFA) had been discovered. Our experiment was to study the anti-CVB3 ability of SFA to cultured heating myocardial cells of CVB3 infected newborn rat. METHODS The myocardial cells were divided into four groups: 1. infected group (n = 8), infected only with CVB3, not adding SFA; 2. treated group (n =8), infected with CVB3, adding SFA (100 microg/ml); 3. drug group (n=6), adding SFA (100 microg/ml) only; 4. control group (n = 6), not infected with CVB3, not adding SFA. RESULTS The myocardial cells of the infected group had cell pathogenic effect (CPE) on the second day after virus inoculation, the CPE progressed rapidly from + to ++++. In contrast, no CPE in the other three groups was found. The LDH and SGOT of the infected group were higher than that in the other three groups, showing a significant difference (P <0.05). The virus titer of the infected group was higher than that of the treated group. There was no influence on normal myocardial cells if the concentration of SFA was lower than 300 microg/ml. When the concentration of SFA was 6.25 microg/ml 200 microg/ml, it showed protective effect on infected myocardial cells. CONCLUSIONS The results of the experiment suggest that SFA might inhibit CVB3 replication in myocardial cells.

Bleeding Outcomes After Dental Extraction in Patients Under Direct-Acting Oral Anticoagulants vs. Vitamin K Antagonists: A Systematic Review and Meta-Analysis.

Abstract: Background: The current systematic review aimed to compare bleeding outcomes in dental extraction patients receiving uninterrupted Direct-acting oral anticoagulant (DOAC) or Vitamin K antagonists (VKAs) for various systemic diseases. Methods: PubMed, Embase, ScienceDirect, CENTRAL, and Google Scholar databases were searched for randomized controlled trials, controlled clinical trials, prospective and retrospective cohort studies, and case control studies, conducted on adult patients undergoing dental extraction under uninterrupted DOAC or VKAs therapy and reporting bleeding outcomes. The search was conducted up to March 31, 2021. We pooled data to calculate risk ratios (RR) with 95% confidence intervals (CI) in a random-effects model. Results: Eight studies comparing 539 patients on DOAC therapy and 574 patients on VKAs were included. Meta-analysis indicated a statistically significant lower bleeding risk in patients under DOAC therapy (RR 0.68 95% CI 0.49, 0.95 I2 = 0%). However, on sensitivity analysis, the results were statistically non-significant after exclusion of any of the included studies. On pooled analysis of limited number of studies, we found no statistically significant difference in the risk of bleeding between apixaban (RR 0.85 95% CI 0.45, 1.60 I2 = 0%), rivaroxaban (RR 0.95 95% CI 0.36, 2.48 I2 = 45%), dabigatran (RR 0.49 95% CI 0.19, 1.28 I2 = 5%), edoxaban (RR 0.41 95% CI 0.13, 1.27 I2 = 0%) and VKAs. Conclusion: The results of the first review comparing bleeding outcomes after dental extraction in patients on uninterrupted DOAC or VKA therapy indicates that patients on DOAC may have a reduced risk of hemorrhage. Current evidence is of very low-quality and should be interpreted with caution. Data on individual DOAC is scarce and at this point, the difference in the risk of bleeding between these drugs cannot be elucidated. Further studies with a large sample size shall supplement our conclusion.