Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

Regulation of osteogenic differentiation by DNA methylation of the dishevelled gene in bone marrow mesenchymal stem cells.

Abstract: Bone marrow mesenchymal stem cells (BMSCs) are stem cells with multidirectional differentiation potential, which can be used as seed cells to repair and reconstruct many types of tissues and organs following injury or disease. Osteogenic differentiation involves a variety of pathway and factors, including cytokines, growth factors, and hormones. In the present study, we investigated the potential role of Dishevelled in osteogenic differentiation of BMSCs in induction medium containing the methyltransferase inhibitor 5-aza-2'-deoxycytidine. The expression of Dishevelled was analyzed using the reverse transcriptase-polymerase chain reaction (RT-PCR) and a Western blot. The methylation degree of the CpG island in the promoter region of the Dishevelled gene was analyzed, and protein expression levels of Wnt, Glycogen synthase kinase-3 (GSK3), axin, Dishevelled, and β-catenin were increased after the addition of the methyltransferase inhibitor. The expression of Dishevelled increased in accordance with the differentiation of osteoblasts, and the degree of methylation of the promoter affected its expression level. In conclusion, regulating the methylation degree of the Dishevelled gene promoter region appears to influence the expression of Dishevelled and therefore the osteogenic differentiation of BMSCs.

Effect of Sheng Xue Ning Tablets on Renal Anemia in Patients Subject to Maintenance Hemodialysis and Safety Evaluation: A Multi-setting Prospective Randomized Study.

Abstract: This study compared Sheng Xue Ning (SXN) tablets with ferrous succinate (FS) tablets in terms of their efficacy for the treatment of iron-deficient renal anemia and safety in patients subject to maintenance hemodialysis (MHD). A total of 94 patients undergoing MHD were randomly assigned to an experiment group (receiving oral SXN tablets, SXN group) and a control group (orally given FS tablets, FS group) and followed up for 12 weeks. Erythropoietin (EPO) was used in both groups. The efficacy was assessed by detecting the subsequent changes in hemoglobin (Hb), serum iron (SI), SF and transferrin saturation (TSAT). At the 12th week, Hb and TSAT levels in both groups were significantly increased compared to those in the screening period (P<0.05). However, no significant difference in Hb and TSAT was found between the two groups. The average weekly EPO dosage used was lower in SXN group than in FS group (P<0.05) at the 10th week and the 12th week. Our study showed that SXN tablets can effectively ameliorate renal anemia and keep iron metabolism stable in MHD patients, and its efficacy is virtually close to that of FS tablets. Meanwhile, SXN tablets can reduce the dosage of EPO and have a good safety profile.

Effect of arachidonic acid and linoleic acid on proliferation of rat hepatic stellate cells

Abstract: AIM To study the effect of arachidonic acid and linoleic acid on proliferation of rat hepatic stellate cells (HSC). METHODS HSCs were isolated and cultured from liver of Wistar rats by in situ perfusion with pronase and collagenase, and density gradient centrifugation with nycodenz. MTT colorimetric assay was detected for HSC proliferation. RESULTS Arachidonic acid and linoleic acid had an effect on proliferation of HSC. Arachidonic acid of 25mg/L promoted HSC proliferation ( P 0 01), but 50 and 100mg/L had an inhibitory effect on HSC, and showed cytotoxity on HSC under inverted microscope; 6 25,12 5 and 25mg/L of linoleic acid had no effect on HSC proliferation, but with concentration increasing, 50 and 100mg/L of linoleic acid might promote HSC proliferation. CONCLUSION Arachidonic acid and linoleic acid may promote HSC proliferation, but increased concentration had cytotoxity on HSC. Arachidonic acid and linoleic acid might be associated with fatty liver and hepatic fibrogenesis by lipid peroxidation.

Expression of leukocyte adhesion molecules CD11b, L-selectin and CD45 during hemodialysis.

Abstract: OBJECTIVE: To examine the gene and protein expression of CD11b, L-selectin and CD45, and the relationship between their expression and leukocytopenia during a hemodialysis session. METHODS: Ten maintenance hemodialysis patients, 20 uremic non-dialysis patients and 10 healthy volunteers were included in this study. The mRNA expression of CD11b, L-selectin and CD45 in peripheral blood mononuclear cells was detected by RT-PCR, while cell surface expression of these molecules on monocytes was detected by flow cytometry and leukocyte number was counted manually. RESULTS: After the start of dialysis, both mRNA expression and cell surface expression of CD11b increased rapidly, while those of L-selectin and leukocyte number fell. The mRNA expression of CD45 first decreased and the cell surface expression increased, followed by a return to pre-dialysis levels, by the end of dialysis. CONCLUSIONS: Hemodialysis using a cuprophane membrane can induce rapid upregulation of CD11b and down-regulation of L-selectin, which might influence the normal adhesion and anti-inflammatory response of leukocytes. In this process CD45 may play a role in regulating and/or transducing activation signal.

Expression of intercellular adhesion molecule 1 by activated hepatic stellate cells

Abstract: AIM To explore the relationship between activation of hepatic stellate cells (HSC) and expression of intercellular adhesion molecule 1(ICAM 1). METHODS HSCs were isolated and cultured from liver of Wistar rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. Expression of ICAM 1 in a quiescent and an activated HSC was detected by immunohistochemistry assay. RESULTS Expression of ICAM 1 was not observed in the quiescent HSC, but seen in the activated, and the amount of the expression was increasing with the time of culture. CONCLUSION Expression of ICAM 1 is related to activation of HSC and liver fibrosis.