Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

In situ PCR and immunohistochemical studies on p16 gene in pituitary adenomas

Abstract: Objective: To examine the occurrence of p16 gene deletion and to analyze p16 expression on paraffin-embedded human pituitary adenoma specimens Efforts were made to optimize the technical conditions forin situ PCR Methods:In situ PCR techniques and inimuno-histochemistry were used Results: Immunohistochemically, p16-positive tumor cells were observed in all cases with various proportions The majority of the stromal cells and part of tumor cells was devoid of p16 immunostaining, but signal ofin situ PCR for p16 gene, exon 2, was displayed in these cells Conclusion: The results implied that p16 gene might not be deleted in these pituitary adenomas It also indicated thatin situ PCR, both direct and indirect methods, proved feasible and informative to the aim of DNA detection It is critical to overcome non-specific amplification in directin situ PCR by means of higher annealing temperature, fewer cycle, lower magnesium concentration and stringent washing A target DNA-deleted sample as the negative control is extremely necessary For the indirect method, the way to improve the sensitivity is to loosen the conditions for amplification and washing, so that more amplification products are subject to hybridization, and signal detection is facilitated

[The relationship between cytochrome P450, subfamily IIIA, polypeptide 5 gene and drug resistance in leukemia cell lines].

Abstract: OBJECTIVE To investigate if the transcription, expression and activities of cytochrome P450, subfamily IIIA, polypeptide 5 gene (CYP3A5) are correlated with drug resistance in leukemia cell lines. METHODS Using RT-PCR, immunohistochemistry and reverse-phase high-performance liquid chromatography (HPLC) assay, CYP3A5 mRNA protein and activities in leukemia cell lines were detected. Daunorubicin (DNR) sensitivity profiles of leukemia cell lines were obtained with MTT assay. Cell cycle characteristics and apoptosis induced by DNR were observed with flow cytometry (FCM) analysis. RESULTS K562, U937, HL-60, NB4 and Jurkat cells were chosen as experimental cell line panels. It was found that CYP3A5 mRNA were measured only in K562 and U937 cells. CYP3A4 and CYP3A7 were not detectable in each cell line. The five leukemia cell lines presented sensitivity to DNR (IC(50), mg/L) in an order as follows: NB4 (0.068 +/- 0.036) > Jurkat (0.076 +/- 0.013) > HL-60 (0.092 +/- 0.016) > K562 (0.148 +/- 0.041) > U937 (0.150 +/- 0.035). Interestingly, K562 and U937 cells (cell lines with CYP3A5 gene transcription) were more resistant to DNR as compared with the other three cell lines in a statistically significant way (P < 0.01). Furthermore, expression and activities of CYP3A5 gene and cell cycle characteristics were observed in K562 and NB4 cells, which represented cell lines with or without CYP3A5 gene transcription respectively. In comparison with NB4 cells, K562 cells expressed CYP3A5 protein and showed a statistically significant higher CYP3A5 activity (3.075 +/- 0.036) x 10(-3) versus (1.635 +/- 0.196) x 10(-3), P < 0.05. FCM analysis showed that S phase cell proportions were similar in K562 and NB4 cells. Incubation with DNR (1 mg/L) for 6 hours induced a remarkable apoptosis peak in NB4 cells, while such peak not occur in K562 cells (apoptosis cell percentage 18.4% and 0.8% respectively). CONCLUSION Only CYP3A5 gene of CYP3A subfamily were detectable in leukemia cell lines, and its transcription, expression and activities were correlated with drug resistance in leukemia cell lines.

[Safety of Helicobacter pylori 'test-and-treat' strategies for the management of uninvestigated dyspepsia]

Abstract: Objective To verify the safety of Helicobacter pylori (Hp) 'test-and-treat' and 'test-and-endoscopy' strategies for management of uninvestigated dyspepsia in Shanghai patients. Methods 14,101 consecutive patients presented with dyspepsia receiving endoscopy during Oct. 2002 to Dec. 2003 were retrospectively analyzed in this study. The detective rate of esophageal or gastroduodenal malignancies and alarm symptoms were investigated and Hp status was assessed. Results Total 202 (1.43%) malignancies were found, including gastric cancer in 162 (1.15%), malignant gastric lymphoma in 4, esophageal cancer in 35 (0.25%) and duodenal cancer in 1. Among those patients with malignancies, ninety-nine (49.0%) patients were infected with Hp and 108 (53.5%) presented with alarm symptoms. Eighteen patients (0.46%) under the age of 45 years were diagnosed as gastric cancer. Among these patients, 5 (27.8%) presented with alarm symptoms and 13 (72.2%) were infected with Hp. If the Hp 'test-and-treat'strategy was used in dyspeptic patients under the age of 45 years without alarm symptoms in Shanghai region, then 13 cases (72.2%) of gastric cancer would be missed. If the Hp 'test-and-endoscopy' strategy was applied, then 3 cases (16.7%) of gastric cancer would be missed. Conclusions Hp 'test-and-treat' and 'test-and-endoscopy' strategies are all not suitable for the management of uninvestigated dyspepsia in Shanghai patients. For most Shanghai adult dyspeptic patients, prompt endoscopy should be recommended as the first-line initial management option.

[The pathological changes of lip biopsies and dental caries on Sjogren's syndrome]

Abstract: This paper showed that in 110 cases of Sjogren's syndrome(SS),the caries incidence was high and was related to the salivary gland lesions.In comparison with 90 matched no SS group,the incidence of caries in SS was 88.18%,and in no SS group 73.33%(P<0.01).The average of caries in cases with more than ten inflammatory foci were significantly increased than in those less than ten,no significant difference in the average of caries was observed between primary and secondary SS.The amounts of salivary excretion measured were significantly decreased in 30 cases with SS than in no SS group cases,and the more the inflammatory foci,the less the salivary excretion.

Correction to: WISP1 and TLR4 on Macrophages Contribute to Ventilator-Induced Lung Injury.

Abstract: Following publication of our article [1], we noticed an error in the “Funding” section