Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

[The effect of PKC-delta inhibitor Rottlerin on human colon cancer cell line SW1116 and its mechanism].

Abstract: Objective To evaluate the effect of PKC-δ inhibitor Rottlerin on human colon cancer cells and its mechanism. Methods Human colon cancer cell line SW1116 cells were treated with Rottlerin. The transcriptional level of DNA methyltransferase （Dnmt）1, Dnmt3a and Dnmt3b was detected by real-time RT-PCR. Cell cycle distribution was evaluated by flow cytometry （FCM）. In addition, cellular morphological changes were examined by light microscopy. Results PKC-δ inhibitor decreased the expression of Dnmtl, Dnmt3a mRNA, up-regulated APC, p21^WAF1 and p16^INK4A mRNA. Demonstarted by flow cytometry, Rottlerin increased the percentage of cell cycle G0/G1 phase cell numbers （ P = 0.02 ） and decreased the percentage of cell cycle GJM phase cell numbers （P = 0.01 ）. Remarkable changes of cellular morphology were observed under light microscope： The volume and cytoplasm of cells treated with Rottlerin were increased. The cell contour was not very clear, and mitotic figures were less frequently seen. Conclusion PKC-δ inhibitor Rottlerin inhibites cell division and proliferation of the colon cancer SW1116 cells through regulating DNA methylation and blocking the signaling pathway of mitogen-activated protein kinase （ MAPK）. Key words: PKC-δ inhibitor; Rottlerin; Human colon cancer cells

Therapeutic effect of kangke injection on viral myocarditis and its anticoxsackie virus mechanism

Abstract: OBJECTIVE To assess the efficacy of Kangke Injection in treating viral myocarditis. METHODS Kangke Injection is the effective ingredient extracted from Radix Sophora flavescens (RSF). Seventy-six cases of virus myocarditis suffering from the continuous positive Coxsackie B virus ribose nucleic acid-polymerase chain reaction (CBVRNA-PCR) in blood, their peak value in blood was determined by high performance liquid chromatography (HPLC) and compared with 50 cases treated by glucose-insulin-potassium chloride (GIK). RESULTS The clearance rate of CBVRNA-PCR and RSF was dose-dependent. The effective rate of RSF on palpitation, chest distress, dispnea was 96.02%, and that of arrhythmia was 100%, all of them were better than those of control. After RSF therapy, the parameters of heart function of ejection fraction (EF), stroke volume (SV), cardiac output (CO), and cardiac index (CI) elevated significantly (P < 0.01), left ventricular mass (LVM) and left ventricular mass index (LVMI) were decreased statistically significantly, while after 5 month therapy, the anti-Coxsackie group B virus neutralizing antibodies of RSF group was returned to normal titer, natural killer (NK) cell activity elevated, P < 0.01. CONCLUSION RSF was an effective substance for regressing the "Pathologic status" of viral myocarditis.

Spiroplakortone, an unprecedented spiroketal lactone from the Chinese sponge Plakortis simplex

Abstract: A new polyketide-based metabolite, spiroplakortone (1), characterized as an unprecedented γ-spiroketal-γ-lactone, was obtained from the Chinese sponge Plakortis simplex. Structural characterization of spiroplakortone (1) was based on extensive spectroscopic analysis and its configuration was partly established by GIAO 13C-NMR, supported by DP4 probability analysis, and ECD calculations. A plausible pathway for the biosynthesis of spiroplakortone (1), envisaging the formation of a carbon–carbon linkage between polyketide and aminoacid (leucine)-derived moieties, has been proposed.

Antisense downregulation of SARS‐CoV gene expression in Vero E6 cells

Abstract: Background Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). It is an enveloped, single-stranded plus-sense RNA virus with a genome of similar to30 kb. The structural proteins E, M and N of SARS-CoV play important roles during host cell entry and vital morphogenesis and release. Therefore, we have studied whether expression of these structural proteins can be down-regulated using an antisense technique. Methods Vero E6 cells were transfected with plasmid constructs containing exons of the SARS-CoV structural protein E, M or N, genes or their exons in frame with the reporter protein EGFP. The transfected cell cultures were treated with antisense phosphorothioated oligonucleotides (antisense PS-ODN, 20mer) or a control oligonucleotide by addition to the culture medium. Results Among a total of 26 antisense PS-ODNs targeting E. N-and N 0 a. genes, we obtained six antisense PS-ODNs which could sequence-specifically reduce target genes expression by over 90% at the concentration of 50 mu,M in the cell culture medium tested by RT-PCR. The antisense effect was proved by down-regulating the expression of the fusion proteins containing the structural proteins E. M or N in frame with the reporter protein EGFP. In Vero E6 cells, the antisense effect was dependent on the concentrations of the antisense PS-ODNs in a range of 0-10 muM or 0-30 muM. Conclusions The antisense PS-ODNs are effective in downregulation of SARS. The findings indicate that antisense knockdown of SARS could be a useful strategy for treatment of SARS, and could also be suitable for studies of the pathological function of SARS genes in a cellular model system. Copyright (C) 2004 John Wiley Sons, Ltd.

Seroepidemiological comparison of Helicobacter pylori infection rates in Shanghai urban districts in 1990 and 2001

Abstract: BACKGROUND: The incidence of diseases related to Helicobacter pylori infection, such as gastric cancer, has been decreasing in developed countries and areas where the economy is rapidly developing. This is also the situation in Shanghai, the largest city in China. However, the prevalent trend of Helicobacter pylori infection in Shanghai is unknown. OBJECTIVE: To investigate changes in the sero-prevalence rate of Helicobacter pylori infection in Shanghai residents between 1990 and 2001. METHODS: We tested serum H. pylori immuno­globulin G antibodies in 1557 blood samples from healthy residents of the Shanghai urban area in 2001 using the ELISA method. The sero-prevalence rate of H. pylori infection in 2001 was compared with that in 1990. RESULTS: In the Shanghai urban area the overall sero-prevalence rate of H. pylori infection in 2001 was 58.3%, higher than that in 1990 (40.5%, P = 0.001). No significant differences in sero-prevalence rates were found in subjects aged 1−9, 10−19 and 20−29 years between 1990 and 2001 (19.8, 42.7, 52.4 vs 27.4, 39.6, and 41.4; P = 0.683, 0.707 and 0.068, respectively). In subjects older than 30 years, a significantly higher prevalence rate of H. pylori infection was observed in 2001 than in 1990 (P < 0.05). CONCLUSION: The prevalence of H. pylori infection in urban Shanghai residents in 2001 was greater than that found in 1990.