Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

Changes in airway responsiveness and relative factors in asthma with remission at puberty.

Abstract: OBJECTIVE To elucidate the airway responsiveness and its relative factors in asthma with natural remission at puberty. METHOD The airway responsiveness was evaluated by provocation of methacholine (PC20). Blood sIL-2R, IL-4 and IFN-gamma values in the cultural supernatant of mononuclear cells, eosinophil count, basophil count and basophil releasability were detected in 18 asthmatic cases with remission at puberty (group I), in 20 asthmatics without remission at puberty (group II), and in 30 healthy controls (group III). RESULT The mean value of PC20 in group I was significantly lower than that in group III (P < 0.01), but still higher than that in group II (P < 0.01). The blood sIL-2R, and eosinophil counts dropped almost to those of group III, significantly lower than those of group II. IL-4 in the culture supernatant of mononuclear cells was similar to group III, but significantly lower than that in group II. However IFN-gamma was also similar to group III, significantly higher than that of group II. The basophil count and positive rate of human basophil degranulation test stimulated by mannitol with hyper-osmolarity were still abnormal, significantly higher than those of group III, and the basophil level was significantly related to the airway responsiveness. CONCLUSION The asthmatics with remission at puberty have a certain degree of airway hyper-responsiveness that is related to the abnormality of basophil.

Reply on RC2

Abstract: An optimal estimation-based algorithm is developed to retrieve number density of excited oxygen (O2) molecules that generate airglow emissions near 0.76 μm (A band) and 1.27 μm (1Δ band) in the upper atmosphere. Both oxygen bands are important for the remote sensing of greenhouse gases. The algorithm is applied to the limb spectra observed by the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) instrument in both nominal (tangent heights below ~90 km) and mesosphere-lower thermosphere (MLT) modes (tangent heights spanning 50–150 km). The number densities of emitting O2 in the 1Δ band are retrieved in an altitude range of 25–100 km near daily in 2010, providing a climatology of O2 1Δ band airglow emission. This climatology will help disentangle airglow from backscattered light in nadir remote sensing of the 1Δ band. The global monthly distributions of the vertical column loading of emitting O2 in 1Δ state show mainly latitudinal dependence without other discernible geographical patterns. Temperature profiles are retrieved simultaneously from the spectral shapes of the 1Δ band airglow emission in the nominal limb mode and from both 1Δ and A band airglow emissions in the MLT mode. The temperature retrievals from both airglow bands are consistent internally and in agreement with independent observations from ACE-FTS and MIPAS with absolute mean bias near or below 5 K and root mean squared error (RMSE) near or below 10 K. The retrieved emitting O2 number density and temperature provide a unique dataset for remote sensing of greenhouse gases and constraining the chemical and physical processes in the upper atmosphere.

Expression of cyclooxygenase‐2 protein in colon disease

Abstract: OBJECTIVE: Several epidemiological studies have indicated that the long-term administration of non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the incidence of colorectal cancer The best known action of NSAIDs is to block cyclooxygenase, the key enzyme required for the conversion of arachidonic acid to prostaglandins Two cyclooxygenase isoforms have been identified and these are referred to as COX-1 and COX-2 Recent studies indicate that inducible COX-2 plays an important role in gastrointestinal inflammation and carcinogenesis The present study was undertaken to investigate the expression and clinical implications of COX-2 and COX-1 in normal and diseased colons METHODS: COX-2 and COX-1 protein expression in specimens from normal controls and diseased colon tissues were examined semiquantitatively by using immunohistochemical methods RESULTS: By using immunohistochemical detection methods, low COX-2 protein expression in colonic epithelial cells was observed in 200% (2/10) of normal controls Eighty percent (16/20) of specimens from inflammatory bowel disease (IBD) had a high COX-2 expression, 4640% (13/28) of adenomas and 643% of (9/14) well-differentiated colonic carcinomas had some COX-2 protein expression Expression of COX-2 protein was increased in IBD and colonic carcinomas compared with normal controls There were no significant differences between colonic adenomas and colonic carcinomas No correlation was found between COX-2 protein expression and patient gender, patient age, tumor size, tumor location or the degree of differentiation/ metastasis of the tumor Strong immunoreactive COX-2 was expressed in clusters in interstitial cells (mainly mononuclear cells) in 536% (15/28) of adenomas and 643% (9/14) of colonic carcinomas Strong COX-2 protein expression was also displayed in the normal glands that were adjacent to the adenomas and carcinomas Expression of COX-1 protein was observed in the epithelial cells and interstitial cells or tumor cells of normal colon, IBD, colonic adenomas and colonic carcinomas CONCLUSIONS: Our results indicated that COX-2 protein overexpression may contribute to the development of IBD and colonic carcinogenesis

Enhancing anti-gastrointestinal cancer activities of CLDN18.2 CAR-T armored with novel synthetic NKG2D receptors Containing DAP10 and DAP12 signaling domains

Abstract: Abstract Chimeric antigen receptor (CAR) T therapies have shown remarkable efficacy in hematopoietic malignancies, but their therapeutic benefits in solid tumors have been limited due to heterogeneities in both antigen types and their expression levels on tumor cells. NK group 2 member D ligands (NKG2DLs) are extensively expressed on various tumors and absent on normal tissues, making them a promising target for cellular immunotherapy. DAP10 and DAP12 function as adaptor proteins in NK cells to transduce activating signals, and recent studies have revealed DAP10 and DAP12’s additional role as a co-stimulatory signal in T cells. Our pre-clinical data showed that CAR-T targeting CLDN18.2 is highly effective in gastrointestinal (GI) cancers, but the heterogeneous expression of CLDN18.2 poses a treatment challenge. To complement this antigen deficiency, we demonstrated that NKG2DLs were extensively expressed in GI tumor tissues and formed an ideal dual target. Here, we reported a CLDN18.2 CAR design armored with synthetic NKG2D receptors (SNR) containing DAP10 and DAP12 signaling domains. This novel CAR-T showed improved cytotoxicity against tumor cells with heterogeneous expression of CLDN18.2. The possible underlined mechanism is that SNR promotes CAR-T memory formation and reduces their exhaustion, while also enhancing their expansion and ability to infiltrate immune-excluded tumors in vivo. Taken together, SNR with DAP10/12 signaling and their synergistic involvement, increased CAR-T function and overcame the antigen deficiency, providing a novel treatment modality for solid GI tumor.