University of Calgary

About: University of Calgary is a education organization based out in Calgary, Alberta, Canada. It is known for research contribution in the topics: Population & Health care. The organization has 44284 authors who have published 104970 publications receiving 3669161 citations. The organization is also known as: U of C & UCalgary.

Growth of Rhodococcus sp. strain BCP1 on gaseous n-alkanes: new metabolic insights and transcriptional analysis of two soluble di-iron monooxygenase genes.

Abstract: Rhodococcus sp. strain BCP1 was initially isolated for its ability to grow on gaseous n-alkanes, which act as inducers for the co-metabolic degradation of low-chlorinated compounds. Here, both molecular and metabolic features of BCP1 cells grown on gaseous and short-chain n-alkanes (up to n-heptane) were examined in detail. We show that propane metabolism generated terminal and sub-terminal oxidation products such as 1- and 2-propanol, whereas 1-butanol was the only terminal oxidation product detected from butane metabolism. Two gene clusters, prmABCD and smoABCD – coding for soluble di-iron monooxgenases (SDIMOs) involved in gaseous n-alkanes oxidation – were detected in the BCP1 genome. By means of reverse transcriptase-quantitative PCR (RT-qPCR) analysis, a set of substrates inducing the expression of the sdimo genes in BCP1 were assessed as well as their transcriptional repression in the presence of sugars, organic acids or during the cell growth on rich medium (Luria Bertani broth). The transcriptional start sites of both the sdimo gene clusters were identified by means of primer extension experiments. Finally, proteomic studies revealed changes in the protein pattern induced by growth on gaseous- (n-butane) and/or liquid (n-hexane) short-chain n-alkanes as compared to growth on succinate. Among the differently expressed protein spots, two chaperonins and an isocytrate lyase were identified along with oxidoreductases involved in oxidation reactions downstream of the initial monooxygenase reaction step.

The Delphi Method for Graduate Research

Abstract: Introduction It continues to be an exciting time to be a researcher in the information systems discipline; there seems to be a plethora of interesting and pressing research topics suitable for research at the masters or PhD level. Researchers may want to look forward to see what will be the key information systems issues in a wireless world, the ethical dilemmas in social network analysis, and the lessons early adopters learn. Practitioners may be interested in what others think about the strengths and weaknesses of an existing information system, or the effectiveness of a newly implemented information system. The Delphi method can help to uncover data in these research directions. The Delphi method is an iterative process used to collect and distill the judgments of experts using a series of questionnaires interspersed with feedback. The questionnaires are designed to focus on problems, opportunities, solutions, or forecasts. Each subsequent questionnaire is developed based on the results of the previous questionnaire. The process stops when the research question is answered: for example, when consensus is reached, theoretical saturation is achieved, or when sufficient information has been exchanged. The Delphi method has its origins in the American business community, and has since been widely accepted throughout the world in many industry sectors including health care, defense, business, education, information technology, transportation and engineering. The Delphi method's flexibility is evident in how it has been used. It is a method for structuring a group communication process to facilitate group problem solving and to structure models (Linstone & Turloff, 1975). The method can also be used as a judgment, decision-aiding or forecasting tool (Rowe & Wright, 1999), and can be applied to program planning and administration (Delbeq, Van de Ven, & Gustafson, 1975). The Delphi method can be used when there is incomplete knowledge about a problem or phenomena (Adler & Ziglio, 1996; Delbeq et al., 1975). The method can be applied to problems that do not lend themselves to precise analytical techniques but rather could benefit from the subjective judgments of individuals on a collective basis (Adler & Ziglio, 1996) and to focus their collective human intelligence on the problem at hand (Linstone & Turloff, 1975). Also, the Delphi is used to investigate what does not yet exist (Czinkota & Ronkainen, 1997; Halal, Kull, & Leffmann, 1997; Skulmoski & Hartman 2002). The Delphi method is a mature and a very adaptable research method used in many research arenas by researchers across the globe. To better understand its diversity in application, one needs to consider the origins of the Delphi method. The Classical Delphi The original Delphi method was developed by Norman Dalkey of the RAND Corporation in the 1950's for a U.S. sponsored military project. Dalkey states that the goal of the project was "to solicit expert opinion to the selection, from the point of view of a Soviet strategic planner, of an optimal U.S. industrial target system and to the estimation of the number of A-bombs required to reduce the munitions output by a prescribed amount," (Dalkey & Helmer, 1963, p. 458). Rowe and Wright (1999) characterize the classical Delphi method by four key features: 1. Anonymity of Delphi participants: allows the participants to freely express their opinions without undue social pressures to conform from others in the group. Decisions are evaluated on their merit, rather than who has proposed the idea. 2. Iteration: allows the participants to refine their views in light of the progress of the group's work from round to round. 3. Controlled feedback: informs the participants of the other participant's perspectives, and provides the opportunity for Delphi participants to clarify or change their views. 4. Statistical aggregation of group response: allows for a quantitative analysis and interpretation of data. …

Guidelines for the management of hemophilia.

Abstract: Hemophilia is a rare disorder that is complex to diagnose and to manage. These evidence-based guidelines offer practical recommendations on the diagnosis and general management of hemophilia, as well as the management of complications including musculoskeletal issues, inhibitors, and transfusion-transmitted infections. By compiling these guidelines, the World Federation of Hemophilia aims to assist healthcare providers seeking to initiate and/or maintain hemophilia care programs, encourage practice harmonization around the world and, where recommendations lack adequate evidence, stimulate appropriate studies.

Trade, Growth and the Environment

Abstract: For the last ten years environmentalists and the trade policy community have engaged in a heated debate over the environmental consequences of liberalized trade. The debate was originally fueled by negotiations over the North American Free Trade Agreement and the Uruguay round of GATT negotiations, both of which occurred at a time when concerns over global warming, species extinction and industrial pollution were rising. Recently it has been intensified by the creation of the World Trade Organization (WTO) and proposals for future rounds of trade negotiations. The debate has often been unproductive. It has been hampered by the lack of a common language and also suffered from little recourse to economic theory and empirical evidence. The purpose of this essay is set out what we currently know about the environmental consequences of economic growth and international trade. We critically review both theory and empirical work to answer three basic questions. What do we know about the relationship between international trade, economic growth and the environment? How can this evidence help us evaluate ongoing policy debates? Where do we go from here?