University of Central Florida

About: University of Central Florida is a education organization based out in Orlando, Florida, United States. It is known for research contribution in the topics: Laser & Population. The organization has 18822 authors who have published 48679 publications receiving 1234422 citations. The organization is also known as: UCF.

Enhanced-alignment Measure for Binary Foreground Map Evaluation

Abstract: The existing binary foreground map (FM) measures to address various types of errors in either pixel-wise or structural ways. These measures consider pixel-level match or image-level information independently, while cognitive vision studies have shown that human vision is highly sensitive to both global information and local details in scenes. In this paper, we take a detailed look at current binary FM evaluation measures and propose a novel and effective E-measure (Enhanced-alignment measure). Our measure combines local pixel values with the image-level mean value in one term, jointly capturing image-level statistics and local pixel matching information. We demonstrate the superiority of our measure over the available measures on 4 popular datasets via 5 meta-measures, including ranking models for applications, demoting generic, random Gaussian noise maps, ground-truth switch, as well as human judgments. We find large improvements in almost all the meta-measures. For instance, in terms of application ranking, we observe improvementrangingfrom9.08% to 19.65% compared with other popular measures.

Multicolor CRISPR labeling of chromosomal loci in human cells

Abstract: The intranuclear location of genomic loci and the dynamics of these loci are important parameters for understanding the spatial and temporal regulation of gene expression. Recently it has proven possible to visualize endogenous genomic loci in live cells by the use of transcription activator-like effectors (TALEs), as well as modified versions of the bacterial immunity clustered regularly interspersed short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we report the design of multicolor versions of CRISPR using catalytically inactive Cas9 endonuclease (dCas9) from three bacterial orthologs. Each pair of dCas9-fluorescent proteins and cognate single-guide RNAs (sgRNAs) efficiently labeled several target loci in live human cells. Using pairs of differently colored dCas9-sgRNAs, it was possible to determine the intranuclear distance between loci on different chromosomes. In addition, the fluorescence spatial resolution between two loci on the same chromosome could be determined and related to the linear distance between them on the chromosome's physical map, thereby permitting assessment of the DNA compaction of such regions in a live cell.

An empirical examination of factors contributing to the creation of successful e-learning environments

Abstract: Although existing models of e-learning effectiveness in information systems (IS) have increased our understanding of how technology can support and enhance learning, most of our models do not take into account the importance of social presence. Thus, this study extends previous research by developing a model of e-learning effectiveness which adds social presence to other oft studied variables including application-specific computer self-efficacy (AS-CSE), perceived usefulness, course interaction, and e-learning effectiveness. Using data from 345 individuals, this model was validated through a field study in an introductory IS survey course. Results indicate that AS-CSE and perceived usefulness were related to course performance, course satisfaction, and course instrumentality. In addition, course interaction was related to course performance and satisfaction. Finally, social presence was related to course satisfaction and course instrumentality. Implications for research and practice are discussed.

Team Training in the Skies: Does Crew Resource Management (CRM) Training Work?:

Abstract: The aviation community has invested great amounts of money and effort into crew resource management (CRM) training. Using D. L. Kirkpatrick's (1976) framework for evaluating training, we reviewed 58 published accounts of CRM training to determine its effectiveness within aviation. Results indicated that CRM training generally produced positive reactions, enhanced learning, and promoted desired behavioral changes. However, we cannot ascertain whether CRM has an effect on an organization's bottom line (i.e., safety). We discuss the state of the literature with regard to evaluation of CRM training programs and, as a result, call for the need to conduct systematic, multilevel evaluation efforts that will show the true effectiveness of CRM training. As many evaluations do not collect data across levels (as suggested by D. L. Kirkpatrick, 1976, and by G. M. Alliger, S. I. Tannenbaum, W. Bennett, Jr., & H. Traver, 1997), the impact of CRM cannot be truly determined; thus more and better evaluations are needed and should be demanded.

Effect of synthesis conditions on the growth of ZnO nanorods via hydrothermal method

Abstract: ZnO nanorods with hexagonal structures were synthesized by a hydrothermal method under different conditions. The effect of synthesis conditions on ZnO nanorod growth was systematically studied by scanning electron microscopy. All samples were characterized by X-ray diffraction, energy-dispersive X-ray spectroscopy and micro-Raman spectroscopy. The results demonstrate that the morphology and ordering of ZnO nanorods are determined by the growth temperature, the overall concentration of the precursors and deposition time. ZnO nanorod morphology and surface-to-volume ratio are most sensitive to temperature. The width of ZnO nanorods can be controlled by the overall concentration of the reactants and by temperature. The influence of the chemical reactions, the nucleation and growth process on the morphology of ZnO nanorods is discussed.