University of Guadalajara

About: University of Guadalajara is a education organization based out in Guadalajara, Mexico. It is known for research contribution in the topics: Population & Context (language use). The organization has 13040 authors who have published 17399 publications receiving 168085 citations. The organization is also known as: UdeG & UdG.

Somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB) in liquid medium and scaled-up in a bioreactor

Abstract: The development of somatic embryos in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB). Explants from immature male flowers were used to form high frequency embryogenic tissue, this tissue was then used to establish embryogenic cell suspensions in a basic MS medium plus 1.0 mg l−1 biotin, 100 mg l−1 glutamine, 100 mg l−1 malt extract (Sigma), 1.0 mg l−1 2,4-D and 45 g l−1 sucrose. Secondary multiplication of somatic embryos was achieved in liquid media in rotary shaker and in bioreactors. The number of embryos per litre obtained with 80.0% DO2 and effects of pH were also studied. A high regeneration percentage of plants were obtained (89.3%) in only 1 month of culture, somatic embryos were then placed to germinate in temporary immersion systems and field testing of somaclonal variation.

Factores de riesgo de dislipidemia en niños y adolescentes con obesidad

Abstract: OBJETIVO: Evaluar el riesgo de dislipidemia asociado a obesidad en ninos y adolescentes. MATERIAL Y METODOS: En un estudio transversal analitico se incluyeron 62 ninos y adolescentes obesos (IMC > centila 95 y pliegue cutaneo tricipital > centila 90) y 70 no obesos (IMC centilas 5-85), con edades de 5 a 15 anos, sin enfermedades cronicas. Se investigaron caracteristicas personales y antecedentes familiares de enfermedades cronicas y se determino el perfil serico de lipidos. Se calculo el riesgo de presentar alteraciones en el perfil serico de lipidos en ninos con obesidad mediante la razon de momios (RM) y se realizo analisis multivariado. RESULTADOS: La edad promedio para todo el grupo fue de 9.8 ± 2.7 anos; 63 individuos eran del sexo femenino y 69 del masculino. La presencia de obesidad se asocio a riesgo de valores anormales de colesterol, trigliceridos, LDL, HDL y dislipidemia (>1 valor anormal) (RM 4.47-15.0). En obesos el analisis multivariado mostro que la pertenencia al sexo femenino se asocio significativamente a dislipidemia. CONCLUSIONES: La obesidad en ninos y adolescentes se asocia a riesgo elevado de presentar dislipidemia; este riesgo es mayor en las mujeres.

Production and characterization of esterase and lipase from Haloarcula marismortui

Abstract: The present study was conducted to investigate the capability of Haloarcula marismortui to synthesize esterases and lipases, and the effect of physicochemical conditions on the growth and the production of esterases and lipases. Finally, the effect of NaCl concentration and temperature on esterase and lipase activities was studied using intracellular crude extracts. In order to confirm the genomic prediction about the esterase and lipase synthesis, H. marismortui was cultured on a rich medium and the crude extracts (intra- or extracellular) obtained were assayed for both activities using p-nitrophenyl esters and triacylglycerides as substrates. Studies on the kinetics of growth and production of esterase and lipase of H. marismortui were performed, reaching a maximum growth rate of 0.053 h−1 and maximal productions of intracellular esterase and lipase of 2.094 and 0.722 U l−1 using p-nitrophenyl valerate and p-nitrophenyl laurate, respectively. Both enzymes were produced as growth-associated metabolites. The effects of temperature, pH, and NaCl concentration on the growth rate and production of enzymes were studied by using a Box–Behnken response surface design. The three response variables were significantly influenced by the physicochemical factors and an interaction effect between temperature and NaCl concentration was also evidenced. The surface response method estimated the following maximal values for growth rate and productions of esterase and lipase: 0.086 h−1 (at 42.5°C, pH 7.4, and 3.6 mol l−1 NaCl), 2.3 U l−1 (at 50°C, pH 7.5, and 4.3 mol l−1 NaCl), and 0.58 U l−1 (at 50°C, pH 7.6, and 4.5 mol l−1 NaCl), respectively. Esterases were active at different salt concentrations, showing two optimal activities (at 0.5 and 5 mol l−1 NaCl), which suggested the presence of two different esterases. Interestingly, in the absence of salt, esterase retained 50% residual activity. Esterases and lipase activities were maximal at 45°C and inactive at 75°C. This study represents the first report evidencing the synthesis of esterase and lipase by H. marismortui.

Clinical, serologic, and immunogenetic features of familial idiopathic inflammatory myopathy

Abstract: Objective To describe the clinical, serologic, and immunogenetic features of familial idiopathic inflammatory myopathy (IIM) and to compare these with the features of sporadic IIM. Methods Clinical signs and symptoms, autoantibodies, HLA-DRB1 and DQA1 alleles, and GM/KM phenotypes were compared among 36 affected and 28 unaffected members of 16 unrelated families in which 2 or more blood relatives developed an IIM. In addition, findings in patients with familial IIM were compared with those in 181 patients with sporadic IIM. The families included 3 pairs of monozygotic twins with juvenile dermatomyositis, 11 families with other siblings or relatives with polymyositis or dermatomyositis, and 2 families with inclusion body myositis. Results The clinical features of familial IIM were similar to those of sporadic IIM, although the frequency of myositis-specific autoantibodies was lower in familial than in sporadic IIM. DRB1*0301 was a common genetic risk factor for familial and sporadic IIM, but contributed less to the genetic risk of familial IIM (etiologic fraction 0.35 versus 0.51 in sporadic IIM). Homozygosity at the HLA-DQA1 locus was found to be a genetic risk factor unique to familial IIM (57% versus 24% of controls; odds ratio 4.2, corrected P = 0.002). Conclusion These findings emphasize that 1) familial muscle weakness is not always due to inherited metabolic defects or dystrophies, but may be the result of the development of IIM in several members of the same family, and 2) multiple genetic factors are likely important in the etiology and disease expression of familial IIM, as is also the case for sporadic myositis, but DQA1 homozygosity is a distinct risk factor for familial IIM.