Institution
University of Manitoba
Education•Winnipeg, Manitoba, Canada•
About: University of Manitoba is a education organization based out in Winnipeg, Manitoba, Canada. It is known for research contribution in the topics: Population & Health care. The organization has 31888 authors who have published 66592 publications receiving 2095493 citations.
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TL;DR: Findings do suggest that Tat neurotoxicity is conformationally dependent, that the active site resides within the first exon of Tat between residues 31 to 61, and that these effects are mediated at least in part by excitatory amino acid receptors.
Abstract: Tat is an 86- to 104-amino-acid viral protein that activates human immunodeficiency virus type 1 expression, modifies several cellular functions, and causes neurotoxicity. Here, we determined the extent to which peptide fragments of human immunodeficiency virus type 1 BRU Tat1-86 produced neurotoxicity, increased levels of intracellular calcium ([Ca2+]i), and affected neuronal excitability. Tat31-61 but not Tat48-85 dose dependently increased cytotoxicity and levels of [Ca2+]i in cultured human fetal brain cells. Similarly, Tat31-61 but not Tat48-85 depolarized rat hippocampal CA1 neurons in slices of rat brain. The neurotoxicity and increases in [Ca2+]i could be significantly inhibited by non-N-methyl-D-aspartate excitatory amino acid receptor antagonists. Shorter 15-mer peptides which overlapped by 10 amino acids each and which represented the entire sequence of Tat1-86 failed to produce any measurable neurotoxicity. Although it remains to be determined if Tat acts directly on neurons and/or indirectly via glial cells, these findings do suggest that Tat neurotoxicity is conformationally dependent, that the active site resides within the first exon of Tat between residues 31 to 61, and that these effects are mediated at least in part by excitatory amino acid receptors.
311 citations
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TL;DR: The gonads of fetal and neonatal mice homozygous for a null mutation in the Gja1 gene encoding Cx43 were examined to determine whether the absence of this connexin has any consequences for gonadal development, and it was demonstrated that folliculogenesis can proceed to the primary (unilaminar) follicle stage in the absent of Cx 43 but that subsequent development is impaired.
Abstract: The connexins are a family of at least 15 proteins that form the intercellular membrane channels of gap junctions. Numerous connexins, including connexin43 (Cx43), have been implicated in reproductive processes by virtue of their expression in adult gonads. In the present study, we examined the gonads of fetal and neonatal mice homozygous for a null mutation in the Gja1 gene encoding Cx43 to determine whether the absence of this connexin has any consequences for gonadal development. We found that in both sexes at the time of birth, the gonads of homozygous mutants were unusually small. This appears to be caused, at least in part, by a deficiency of germ cells. The germ cell deficiency was traced back as far as Day 11.5 of gestation, implying that it arises during early stages of germ line development. We also used an organ culture technique to examine postnatal folliculogenesis in the mutant ovaries, an approach necessitated by the fact that Gja1 null mutant offspring die soon after birth because of a heart abnormality. The results demonstrated that folliculogenesis can proceed to the primary (unilaminar) follicle stage in the absence of Cx43 but that subsequent development is impaired. In neonatal ovaries of normal mice, Cx43 could be detected in the somatic cells as early as Day 1, when primordial follicles begin to appear, supporting the conclusion that this connexin is required for the earliest stages of folliculogenesis. These results imply that gap junctional coupling mediated by Cx43 channels plays indispensable roles in both germ line development and postnatal folliculogenesis.
311 citations
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TL;DR: This work reports that B. cinerea secretes a virulence factor that hijacks the plant’s own crosstalk network to promote disease development, and shows that the JA signaling pathway required for tomato resistance against B.cinerea is mediated by the systemin elicitor.
Abstract: Plants have evolved sophisticated mechanisms to sense and respond to pathogen attacks. Resistance against necrotrophic pathogens generally requires the activation of the jasmonic acid (JA) signaling pathway, whereas the salicylic acid (SA) signaling pathway is mainly activated against biotrophic pathogens. SA can antagonize JA signaling and vice versa. Here, we report that the necrotrophic pathogen Botrytis cinerea exploits this antagonism as a strategy to cause disease development. We show that B. cinerea produces an exopolysaccharide, which acts as an elicitor of the SA pathway. In turn, the SA pathway antagonizes the JA signaling pathway, thereby allowing the fungus to develop its disease in tomato (Solanum lycopersicum). SA-promoted disease development occurs through Nonexpressed Pathogen Related1. We also show that the JA signaling pathway required for tomato resistance against B. cinerea is mediated by the systemin elicitor. These data highlight a new strategy used by B. cinerea to overcome the plant’s defense system and to spread within the host.
311 citations
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TL;DR: The Patient Dignity Inventory is a valid and reliable new instrument, which could assist clinicians to routinely detect end-of-life dignity-related distress, and should help clinicians deliver quality, dignity-conserving end- of-life care.
310 citations
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TL;DR: Prostaglandin synthesis, glutamate release, histamine receptors, and visceral afferents represent functional biochemical and neural pathways through which endotoxin activates c-fos protein in specific autonomic and neuroendocrine regulatory nuclei.
310 citations
Authors
Showing all 32123 results
Name | H-index | Papers | Citations |
---|---|---|---|
George Davey Smith | 224 | 2540 | 248373 |
Peer Bork | 206 | 697 | 245427 |
David A. Weitz | 178 | 1038 | 114182 |
Yang Yang | 171 | 2644 | 153049 |
Robert E. W. Hancock | 152 | 775 | 88481 |
Peter B. Jones | 145 | 1857 | 94641 |
Peter Lang | 140 | 1136 | 98592 |
James J. Gross | 139 | 529 | 100206 |
Steven J.M. Jones | 137 | 594 | 146609 |
Rajkumar Buyya | 133 | 1066 | 95164 |
Jeff A. Sloan | 129 | 656 | 65308 |
Dafna D. Gladman | 129 | 1036 | 75273 |
Murray B. Stein | 128 | 745 | 89513 |
Robert W. Heath | 128 | 1049 | 73171 |
Jürgen Rehm | 126 | 1132 | 116037 |