Showing papers by "University of Oviedo published in 1990"
TL;DR: The causative viral agent of a lethal rabbit hemorrhagic disease has been purified and characterized and the major structural protein was 60 kilodaltons, which constitutes a unique characteristic of the Caliciviridae.
Abstract: The causative viral agent of a lethal rabbit hemorrhagic disease has been purified and characterized. In negative-stained preparations, the virions were icosahedral, measured 27 to 35 nm in diameter, were without an envelope, and showed 10 peripheral cup-shaped depressions. The major structural protein was 60 kilodaltons, which constitutes a unique characteristic of the Caliciviridae.
194 citations
TL;DR: The main clinical features of SEB were high frequency in male patients greater than 40 years of age, fever, leukocytosis, anemia, and phlebitis, and several potentially toxic exoproteins were detected.
Abstract: Staphylococcus epidermidis was established as the causal agent of true bacteremia on the basis of microbiologic, epidemiologic, clinical, and prognostic data on 65 episodes of S. epidermidis bacteremia (SEB) recorded in the Hospital Covadonga of Oviedo, Spain, during 1982-1986. All isolates were susceptible in vitro to vancomycin, mercury, silver, cadmium, and malachite green, and 95.2% of isolates were susceptible to cephalothin; a dominant biotype, four resistotypes (ion-dye resistance patterns), and four antibiotypes (antibiotic resistance patterns) were found. A high percentage of isolates (76.7%) produced slime, and several potentially toxic exoproteins were detected in various and generally lower proportions. The main clinical features of SEB were high frequency in male patients greater than 40 years of age, fever, leukocytosis, anemia, and phlebitis. Indwelling devices were the most common portal of entry. The overall death rate was 36.9%. Underlying disease, hemodynamic status, predisposing factors, and treatment were statistically significant parameters in relation to mortality.
115 citations
TL;DR: In this paper, an upwelling process taking place in the season of the thermal stratification which is persistent throughout the summer was detected near the surface in some locations off the Central Cantabrian Coast (Bay of Biscay).
Abstract: North Atlantic Central Waters were detected near the surface in some locations off the Central Cantabrian Coast (Bay of Biscay). This feature suggests an upwelling process taking place in the season of the thermal stratification which is persistent throughout the summer. The exact mechanism of formation is unknown but the wind driven currents are probably the main force. The prevailing winds off the Cantabrian coast are northeast during summer, generating westward superficial currents that produce an Eckman transport offshore. Factors such as shape of the coast and slope topography maybe modulators. Although the upwelling was subsuperficial, our results suggest that cold and nutrient rich waters reached the surface previously. The biomass distribution of phytoplankton varied according to the upwelling and different populations could be recognized. In stations not affected by upwelling phytoplankton occured mainly on top of the thermocline, whereas in those affected by upwelled waters it was concentrated near the surface. The mean size and composition of the populations were also different, and two distinct types could be recognized and related to nutrient concentrations.
108 citations
TL;DR: Two previously described mussel pump characteristics initiated in response to increases in ambient flow pressure — pump shutdown or gill bypass shunting — are possible explanations for feeding inhibition in the blue mussel.
104 citations
TL;DR: A possible role of apolipoprotein D in progesterone transport is proposed after it was found that all the sequenced tryptic peptides from protein GCDFP-24 matched perfectly with regions present in the amino acid sequence determined for human apoliprotein D.
Abstract: GCDFP(gross-cystic-disease-fluid protein)-24, a progesterone-binding protein present in large amounts in cyst fluid from human breast gross cystic disease, was purified in a one-step procedure by size-exclusion h.p.l.c. Peptide fragments obtained by trypsin digestion of the intact protein were purified by reverse-phase h.p.l.c. and analysed for their amino acid composition and subjected to automated Edman degradation. A search of the National Biomedical Research Foundation Data Bank revealed that all the sequenced tryptic peptides from protein GCDFP-24 matched perfectly with regions present in the amino acid sequence determined for human apolipoprotein D. Additional data on N-terminal sequence of the unblocked proteins, carbohydrate-attachment sites, amino acid composition and molecular-mass estimations supported the identity between both molecules. On the basis of this identity a possible role of apolipoprotein D in progesterone transport is proposed.
103 citations
TL;DR: The most remarkable ideas suggested by this crystal simulation are the following: the crystal potential produces a contraction of the free-ion valence radial density that works as a bonding mechanism able to describe accurately the stability and equilibrium elastic constants of simple ionic crystals.
Abstract: A new theoretical scheme appropriate for studying the electronic structure of ionic crystals is presented and applied to nine AB-type cubic lattices (A=Li,Na,K; B=H,F,Cl). The scheme, called the ab initio perturbed-ion (PI) method, is based on the theory of electronic separability and the ab initio model-potential approach of Huzinaga. In the PI method, the self-consistent-field (SCF) equations for each different lattice ion are first solved in a lattice potential that contains nuclear attraction, Coulombic, and nonlocal exchange operators, and lattice projectors enforcing the required ion-lattice orthogonality. The ionic SCF solutions are then used to compute the lattice potential, and the process is repeated until ion-lattice consistency is achieved. The lattice energy and other equilibrium properties are immediately obtained from the PI wave functions. The most remarkable ideas suggested by this crystal simulation are the following: (a) The crystal potential produces a contraction of the free-ion valence radial density, large for the anions but very small for the cations, that works as a bonding mechanism able to describe accurately the stability and equilibrium elastic constants of simple ionic crystals; (b) the PI method explains well the variation of several crystal properties with hydrostatic pressure; (c) the crystal bonding can be clearly analyzed in terms of simple cationic and anionic contributions. Moreover, the PI code may be used as an efficient source of environment-consistent ionic wave functions and energies.
97 citations
TL;DR: Results confirmed that the homoeologous relationships between chromosome arms of the A and B genomes in T. turgidum are the same as inT.
Abstract: Meiotic pairing was analyzed at metaphase I in ABRR triticale × rye hybrids to identify the arm homoeology of chromosomes ofTriticum turgidum Wheat chromosomes were identified using a C-banding technique Results confirmed that the homoeologous relationships between chromosome arms of the A and B genomes inT turgidum are the same as inT aestivum, and that a double translocation involving4AL,5AL, and7BS is present inT turgidum It is proposed that a pericentric inversion involving a substantial portion of chromosome4A is present inT turgidum andT aestivum
73 citations
TL;DR: The influence of different nutritional compounds on oleandomycin biosynthesis by Streptomyces antibioticus was studied, resulting in the design of a chemically defined medium for production of the antibiotic, showing a triphasic pattern of resistance.
Abstract: Summary: The influence of different nutritional compounds on oleandomycin biosynthesis by Streptomyces antibioticus was studied, resulting in the design of a chemically defined medium for production of the antibiotic. Of the variety of carbon and nitrogen compounds tested, fructose and aspartic acid (carbon and nitrogen sources, respectively) supported the highest oleandomycin titres. Addition of propionate but not acetate, both precursors of the skeleton of the macrolide lactone ring, stimulated the biosynthesis of the antibiotic. Oleandomycin biosynthesis was repressed by glucose but not by phosphate. S. antibioticus develops oleandomycin resistance shortly before the antibiotic begins to be synthesized, showing a triphasic pattern of resistance: spores and producing mycelium are resistant, while non-producing mycelium is sensitive.
73 citations
TL;DR: In this paper, an initial concentration of molybdenum between 1 and 10g 1−1 and tungsten between 5 and 10 g 1 −1 as a function of initial pH, organic/aqueous phase volume ratio and tengsten/molybensen ratio was obtained.
66 citations
TL;DR: The gene encoding protein p72, the major structural protein of African swine fever virus and one of the most immunogenic proteins in natural infection has been mapped and sequenced and contains the coding information for all the sequenced tryptic peptides fromprotein p72.
64 citations
TL;DR: In this paper, a succession of stress regimes was applied to the same three major fault systems, NE-SW, NW-SE and E-W, which were inherited from the Variscan Orogeny, some of them having already been involved in the latest Stephanian-Early Permian rifting episode.
TL;DR: A flow-injection analysis (FIA) system utilizing a horseradish peroxidase-modified amperometric electrode is described, and the reduction current was found to be proportional to the concentration of hydrogen peroxide.
Abstract: A flow-injection analysis (FIA) system utilizing a horseradish peroxidase-modified amperometric electrode is described. The enzyme was immobilized through adsorption onto a glassy carbon electrode, and the system is used to determine hydrogen peroxide at submicromolar levels. The response is based on the enzymatic reduction of hydrogen peroxide in the presence of hydroquinone acting as an electron mediator. The oxidized quinone produced in the enzymatic reaction is reduced at the electrode, and the reduction current was found to be proportional to the concentration of hydrogen peroxide. Calibration curves were found to be linear between 2.5 × 10−8 and 1.0 × 10−6 M, and the method has a detection limit of 1.0 × 10−8 M.
TL;DR: A method to isolate the major allergen from olive pollen (Ole e I) in high yield by reverse-phase HPLC and the amino acid sequence of the first 27 amino acid residues from the N-terminal end is given.
TL;DR: D. magnitorata exhibited greater digestive efficiency when it was fed with the algae for which the isopod showed strong preference, and this result suggests that factors other than food quality might be important in food selection by D. bidentata, particularly availability of the resource in time and space.
TL;DR: The production of extracellular protease by Streptomyces clavuligerus was strongly influenced by the nature of the nitrogen source and was related to the growth rates of the cultures and was stimulated by low concentrations of casamino acids or yeast extract.
Abstract: The production of extracellular protease by Streptomyces clavuligerus was strongly influenced by the nature of the nitrogen source. Production took place in batch cultures during growth at low or intermediate growth rates, but was delayed to the post-exponential phase in media supporting high growth rates. Protease formation could be initiated by a nutritional shift-down induced by casamino acids deprivation. Under both types of conditions maximal production was related to the growth rates of the cultures and was stimulated by low concentrations of casamino acids or yeast extract. Some purine compounds also influenced production in shift-down conditions. Ammonium interfered with protease formation whenever it was added to the medium. Some mutants with ltered nitrogen control of primary metabolism were also affected in the production of protease. A partial characterization of the activity indicated that it was due to a single metalloprotease with an apparent molecular mass of 41,700 Da.
TL;DR: In this article, n-Alkylamines have been intercalated by α-titanium phosphate by exposing the solid to the vapour of the amines, and X-ray diffraction patterns and information on the disposition of the guests within the interlayer region have been derived.
TL;DR: The effect of sinefungin and SAH on several Streptomyces methyltransferases (DNA and protein MTases) and on other bacterial DNA-MTases is described.
Abstract: Sinefungin is a naturally occurring nucleoside isolated from cultures of Streptomyces griseolus and S. incarnatus. It is structurally related to S-adenosyl-methionine (SAM) and S-adenosyl-l-homocysteine (SAH). Its effect and level of action on prokaryotes has not been studied with the same detail as with eukaryotic cells. In this report we describe the effect of sinefungin and SAH on several Streptomyces methyltransferases (DNA) and protein MTases) and on other bacterial DNA-MTases. Protein Mtases are resistant to sinefungin, whereas DNA-MTases are inhibited. Adenine MTases however, seem more sensitive to this analogue than cytosine MTases.
TL;DR: Results indicate that three months cold storage greatly increases morphogenic capacity and reduces contamination and oxidation of tissues, giving rise to a higher microplant production than the one obtained using previously described methods.
Abstract: Apical buds (0.5 cm) and nodal shoot segments (1.5 cm) excised from: A) field-grown branches, B) newly developed shoots from the forced outgrowth of axillary buds on A branches, C) newly developed shoots from the forced outgrowth of axillary buds on A branches submitted to cold storage were used as primary explants. Results indicate that three months cold storage greatly increases morphogenic capacity and reduces contamination and oxidation of tissues. Consequently, a multiplying chain could be easily established by culturing the tissues on a modified Murashige & Skoog (1962) medium plus 6-benzyl-aminopurine 5 mg l-1, indole-3-acetic acid 0.01 mg l-1 and gibberellic acid 0.1 mg l-1. During the initiation and proliferation phases, both the proliferation and the elongation rate were significantly increased when a double-phase culture system (Viseur 1987) was used, giving rise to a higher microplant production than the one obtained using previously described methods. Plant regeneration was achieved by immersing the single microshoot's basal end in an IBA (0.1–1 mg ml-1) solution for 10 s followed by a 20-day culture on a 1/2 MS2 medium.
TL;DR: The similarity scores obtained after comparison of known thymidine kinase sequences indicated that the African swine fever virus thymine kinase is more distantly related than the poxvirus thymazine kinases to their cellular homologs.
TL;DR: A glutathione S‐transferase from Escherichia coli has been purified approximately 800‐fold with an 11% activity yield by passage through DEAE Sephacel and glutathion‐agarose affinity columns.
TL;DR: In this paper, the reaction of N-vinylic λ5-phosphazenes with acyl halides followed by addition of nucleophiles lead to 1-donor substituted 3-alkoxycarbonyl-2-aza-1,3-butadienes with good to excellent yields.
TL;DR: Aluminium was eluted as a single peak in the same fraction as transferrin, however, following the addition of desferrioxamine most of the aluminium was liberated from transferrin and become attached to the chelator.
TL;DR: The extensive meristematic activities observed indicated the enormous morphological potential of this species.
Abstract: A twenty fold multiplication per twenty days of caper was achieved by culturing nodal shoot segments in the presence of BAP (4 μM) plus IAA (03 μM) and GA3 (03 μM) The use of a modified MS medium facilitated this response Plantlet regeneration was induced on single shoots taken from proliferating clusters subcultured for 20 days on a reduced BAP (2 μM) without auxin and gibberellin Higher rooting responses (70%) were obtained after a 20-day incubation period in darkness on solid half-strength MS1 medium plus IAA (30 μM), followed by a subsequent 20 day culture period on half-strength MSI basal medium Proliferation was mainly due to axillary shoot-bud development as revealed by histological studies The extensive meristematic activities observed indicated the enormous morphological potential of this species
TL;DR: The degree of heterozygosity of five enzymatic loci is related to the degree of fluctuating asymmetry of three bilateral traits in two farm stocks and in two natural populations of Salmo salar to detect an inverse correlation with the number of asymmetrical traits per individual and with the total magnitude of asymmetry.
TL;DR: In this paper, the oxidation of heroin on a carbon paste electrode has been studied by using voltammetric techniques under both semi-infinite linear diffusion and hydrodynamic conditions.
Abstract: The oxidation of heroin on a carbon paste electrode has been studied by using voltammetric techniques under both semi-infinite linear diffusion and hydrodynamic conditions. By employing a simple and rapid in situ pre-treatment of the electrode, excellent reproducibility of the current signal was obtained. Subsequently, the current was measured and the concentration of total heroin present could be determined. The method compared favourably with instrumental methods that are more commonly used for the quantification of heroin in clinical laboratories such as chromatographic, spectrophotometric or radiometric techniques. Moreover, the proposed method showed good selectivity and was superior with respect to cost and time of analysis, permitting the determination of heroin in seized drug samples over a wide concentration range.
TL;DR: In this article, a simple and rapid method for the determination of the antibiotic ciprofloxacin by differential pulse polarography (DPP) has been developed, and the changes in the voltammetric behavior of this drug compound with pH was studied.
Abstract: A simple and rapid method for the determination of the antibiotic ciprofloxacin by differential pulse polarography (DPP) has been developed. The changes in the voltammetric behavior of this drug compound with pH was studied, and the most suitable pH for analytical purposes was found to be pH 8.5. The DPP behavior of this compound at this pH exhibited two reduction waves at −1.44 and −1.64 V. The former wave could be used to determine ciprofloxacin between 5 × 10−7 and 3 × 10−5 M. The method was applied to the determination of ciprofloxacin in formulated tablets with a relative standard deviation of less than 0.4%.
TL;DR: In this paper, the mixing and segregation conditions of wood sawdust and coal particles in a fluidized bed of sand or alumina particles were established at room temperature under atmospheric pressure.
Abstract: This paper presents an experimental study in order to establish mixing and segregation conditions of wood sawdust and coal particles in a fluidized bed of sand or alumina particles. Experiments were performed at room temperature under atmospheric pressure. Simple equipment was used to divide the bed into layers and useful results could be obtained in a short period of time. The influence of fluidization velocity, time of an experiment, size and concentration of sawdust or coal particles was investigated.
Uniformity of mixing was found to increase with an increase in the fluidization velocity. For u/umf less than 2.5, strong segregation occurred between sawdust and sand particles.
Determination of good mixing conditions will be useful in gasification studies of sawdust and coal.
TL;DR: In this paper, a rapid and sensitive method for the simultaneous determination of primary amino acids in apple is described, where amino acids were derivatized with o-phthaldialdehyde/2-mercaptoethanol and separated on a reversed phase column with a gradient of phosphate buffer-tetrahydrofuran-methanol as the mobile phase.
Abstract: A rapid and sensitive method for the simultaneous determination of primary amino acids in apple is described. After sample preparation, amino acids were derivatized with o-phthaldialdehyde/2-mercaptoethanol and separated on a reversed phase column with a gradient of phosphate buffer-tetrahydrofuran-methanol as the mobile phase. Detection was carried out with a fluorescence detector at excitation and emission wavelengths of 340 nm and 425 nm respectively. Recovery studies showed good results for all substances (91–109%) (with coefficients of variation ranging, from 0.1 to 9.0%). This method was applied to the monitoring of amino acids during the ripening of apples.
TL;DR: Nonstructural proteins of plum pox potyvirus were partially purified following a procedure described for the isolation of tobacco etch virus nuclear inclusion proteins.