Showing papers by "University of Salford published in 2005"

Interleukin-1 and neuronal injury

Abstract: Interleukin-1 is a pro-inflammatory cytokine that has numerous biological effects, including activation of many inflammatory processes (through activation of T cells, for example), induction of expression of acute-phase proteins, an important function in neuroimmune responses and direct effects on the brain itself. There is now extensive evidence to support the direct involvement of interleukin-1 in the neuronal injury that occurs in both acute and chronic neurodegenerative disorders. This article discusses the key evidence of a role for interleukin-1 in acute neurodegeneration - for example, stroke and brain trauma - and provides a rationale for targeting the interleukin-1 system as a therapeutic strategy.

TGF-β1 induces human alveolar epithelial to mesenchymal cell transition (EMT)

Abstract: Fibroblastic foci are characteristic features in lung parenchyma of patients with idiopathic pulmonary fibrosis (IPF). They comprise aggregates of mesenchymal cells which underlie sites of unresolved epithelial injury and are associated with progression of fibrosis. However, the cellular origins of these mesenchymal phenotypes remain unclear. We examined whether the potent fibrogenic cytokine TGF-β1 could induce epithelial mesenchymal transition (EMT) in the human alveolar epithelial cell line, A549, and investigated the signaling pathway of TGF-β1-mediated EMT. A549 cells were examined for evidence of EMT after treatment with TGF-β1. EMT was assessed by: morphology under phase-contrast microscopy; Western analysis of cell lysates for expression of mesenchymal phenotypic markers including fibronectin EDA (Fn-EDA), and expression of epithelial phenotypic markers including E-cadherin (E-cad). Markers of fibrogenesis, including collagens and connective tissue growth factor (CTGF) were also evaluated by measuring mRNA level using RT-PCR, and protein by immunofluorescence or Western blotting. Signaling pathways for EMT were characterized by Western analysis of cell lysates using monoclonal antibodies to detect phosphorylated Erk1/2 and Smad2 after TGF-β1 treatment in the presence or absence of MEK inhibitors. The role of Smad2 in TGF-β1-mediated EMT was investigated using siRNA. The data showed that TGF-β1, but not TNF-α or IL-1β, induced A549 cells with an alveolar epithelial type II cell phenotype to undergo EMT in a time-and concentration-dependent manner. The process of EMT was accompanied by morphological alteration and expression of the fibroblast phenotypic markers Fn-EDA and vimentin, concomitant with a downregulation of the epithelial phenotype marker E-cad. Furthermore, cells that had undergone EMT showed enhanced expression of markers of fibrogenesis including collagens type I and III and CTGF. MMP-2 expression was also evidenced. TGF-β1-induced EMT occurred through phosphorylation of Smad2 and was inhibited by Smad2 gene silencing; MEK inhibitors failed to attenuate either EMT-associated Smad2 phosphorylation or the observed phenotypic changes. Our study shows that TGF-β1 induces A549 alveolar epithelial cells to undergo EMT via Smad2 activation. Our data support the concept of EMT in lung epithelial cells, and suggest the need for further studies to investigate the phenomenon.

Developing a robust part-of-speech tagger for biomedical text

Abstract: This paper presents a part-of-speech tagger which is specifically tuned for biomedical text We have built the tagger with maximum entropy modeling and a state-of-the-art tagging algorithm The tagger was trained on a corpus containing newspaper articles and biomedical documents so that it would work well on various types of biomedical text Experimental results on the Wall Street Journal corpus, the GENIA corpus, and the PennBioIE corpus revealed that adding training data from a different domain does not hurt the performance of a tagger, and our tagger exhibits very good precision (97% to 98%) on all these corpora We also evaluated the robustness of the tagger using recent MEDLINE articles

Relationships between trauma and psychosis: an exploration of cognitive and dissociative factors.

Abstract: Objective: There is a growing awareness of the relationship between trauma and psychosis. Studies have found associations between traumatic life experiences and the development of psychosis. Method: This study examined the prevalence of trauma exposure and post-traumatic stress disorder (PTSD) in 32 people with psychotic diagnoses and investigated associations between specific traumas and psychotic symptoms. The role of dissociation and cognitive factors was also explored. Results: The prevalence of lifetime trauma was high (94% of the sample reported at least one traumatic event). The prevalence of current PTSD was 53%. Severity of trauma was associated with severity of PTSD and psychotic experiences. Physical abuse was associated with positive psychotic symptoms and sexual abuse was specifically related to hallucinations. Dissociative processes and negative beliefs formed as a result of trauma were associated with psychotic experiences (particularly hallucinations). Conclusion: These findings are consistent with the suggestion that psychosis may be trauma-induced, and other implications are considered.

On the categorization of demand patterns.

Abstract: The categorization of alternative demand patterns facilitates the selection of a forecasting method and it is an essential element of many inventory control software packages. The common practice in the inventory control software industry is to arbitrarily categorize those demand patterns and then proceed to select an estimation procedure and optimize the forecast parameters. Alternatively, forecasting methods can be directly compared, based on some theoretically quantified error measure, for the purpose of establishing regions of superior performance and then define the demand patterns based on the results. It is this approach that is discussed in this paper and its application is demonstrated by considering EWMA, Croston's method and an alternative to Croston's estimator developed by the first two authors of this paper. Comparison results are based on a theoretical analysis of the mean square error due to its mathematically tractable nature. The categorization rules proposed are expressed in terms of the average inter-demand interval and the squared coefficient of variation of demand sizes. The validity of the results is tested on 3000 real-intermittent demand data series coming from the automotive industry.

Text mining and ontologies in biomedicine: Making sense of raw text

Abstract: The volume of biomedical literature is increasing at such a rate that it is becoming difficult to locate, retrieve and manage the reported information without text mining, which aims to automatically distill information, extract facts, discover implicit links and generate hypotheses relevant to user needs. Ontologies, as conceptual models, provide the necessary framework for semantic representation of textual information. The principal link between text and an ontology is terminology, which maps terms to domain-specific concepts. This paper summarises different approaches in which ontologies have been used for text-mining applications in biomedicine.

Remobilization of southern African desert dune systems by twenty-first century global warming

Abstract: Desert dunes cover some 5% of Earth's land surface, including almost 30% of Africa, where vegetated dunes are widely used in agriculture, but the potential impact of global warming on dune systems is not well understood. New climate modelling studies suggest that for a range of CO2 emissions scenarios, extensive dune systems from South Africa to Angola will be activated as a consequence of future global warming by 2010. This shift in desert dunes could have drastic effects on the environment, and marked socio-political implications for the countries in which the dune systems fall. Although desert dunes cover 5 per cent of the global land surface and 30 per cent of Africa, the potential impacts of twenty-first century global warming on desert dune systems are not well understood1. The inactive Sahel and southern African dune systems, which developed in multiple arid phases since the last interglacial period2, are used today by pastoral and agricultural systems3,4 that could be disrupted if climate change alters twenty-first century dune dynamics. Empirical data and model simulations have established that the interplay between dune surface erodibility (determined by vegetation cover and moisture availability) and atmospheric erosivity (determined by wind energy) is critical for dunefield dynamics5. This relationship between erodibility and erosivity is susceptible to climate-change impacts. Here we use simulations with three global climate models and a range of emission scenarios to assess the potential future activity of three Kalahari dunefields. We determine monthly values of dune activity by modifying and improving an established dune mobility index6 so that it can account for global climate model data outputs. We find that, regardless of the emission scenario used, significantly enhanced dune activity is simulated in the southern dunefield by 2039, and in the eastern and northern dunefields by 2069. By 2099 all dunefields are highly dynamic, from northern South Africa to Angola and Zambia. Our results suggest that dunefields are likely to be reactivated (the sand will become significantly exposed and move) as a consequence of twenty-first century climate warming.

Attention to pain localization and unpleasantness discriminates the functions of the medial and lateral pain systems.

Abstract: Functional imaging studies have identified a matrix of structures in the brain that respond to noxious stimuli. Within this matrix, a division of function between sensory-discriminative and affective responses has so far been demonstrated by manipulating either pain intensity or unpleasantness under hypnosis in two different normal volunteer groups studied on separate occasions. Our study used positron emission tomography (PET) to demonstrate this division of function under more natural conditions in a healthy group of volunteers, using a CO(2) laser to provide nociceptive stimuli that selectively activate A-delta and C-fibres without contamination by touch sensations. We measured the differential cerebral responses to noxious and innocuous laser stimuli during conditions of selective attention to either the unpleasantness or location of the stimuli. Attention to location increased responses in the contralateral (right) primary somatosensory and inferior parietal cortices. This result implies that these components of the lateral pain system are concerned mainly with the localization of pain. In contrast, attention to unpleasantness increased responses in bilateral perigenual cingulate and orbitofrontal cortices, contralateral (right) amygdala, ipsilateral (left) hypothalamus, posterior insula, M1 and frontal pole. These areas comprise key components of the medial pain and neuroendocrine systems and the results suggest that they have a role in the affective response to pain. Our results indicate the importance of attentional effects on the pattern of nociceptive processing in the brain. They also provide the first clear demonstration, within a single experiment, of a major division of function within the neural pain matrix.

Satellite remote sensing of forest resources: three decades of research development

Abstract: Three decades have passed since the launch of the first international satellite sensor programme designed for monitoring Earth’s resources. Over this period, forest resources have come under increasing pressure, thus their management and use should be underpinned by information on their properties at a number of levels. This paper provides a comprehensive review of how satellite remote sensing has been used in forest resource assessment since the launch of the first Earth resources satellite sensor (ERTS) in 1972. The use of remote sensing in forest resource assessment provides three levels of information; namely (1) the spatial extent of forest cover, which can be used to assess the spatial dynamics of forest cover; (2) forest type and (3) biophysical and biochemical properties of forests. The assessment of forest information over time enables the comprehensive monitoring of forest resources. This paper provides a comprehensive review of how satellite remote sensing has been used to date and, building on...

British Association of Dermatologists guidelines for use of biological interventions in psoriasis 2005.

Abstract: Psoriasis is a common, persistent, relapsing inflammatory skin disease that can be associated with significant morbidity. Quality of life studies in psoriasis reveal a negative impact on patients comparable with that seen in cancer, arthritis and heart disease.1–5 Patients with severe disease constitute approximately 20–30% of all patients with psoriasis, often require systemic treatment, and represent a major economic burden to the Health Service. All standard systemic therapies for severe disease are associated with the potential for major long-term toxicity, many are expensive, and a proportion of patients has treatmentresistant disease.6 Biological therapies or ‘biologics’ describe agents designed to block specific molecular steps important in the pathogenesis of psoriasis and have emerged over the last 3–5 years as potentially valuable alternative therapeutic options. Currently, biological therapies for psoriasis comprise two main groups: (i) agents targeting the cytokine tumour necrosis factor (TNF)-a (e.g. etanercept, infliximab, adalimumab) and (ii) agents targeting T cells or antigen-presenting cells (e.g. efalizumab, alefacept). Two of these, etanercept (Enbrel) and efalizumab (Raptiva) were licensed in 2004 in the U.K. for patients with moderate to severe psoriasis.

Fearnot!: an experiment in emergent narrative

Abstract: We discuss the experience of constructing the application FearNot! (Fun with Empathic Agents Reaching Novel Outcomes in Teaching), an application of virtual drama to anti-bullying education inspired by Forum Theatre. An appraisal-driven agent architecture is presented as a mechanism for generating an emergent, that is, unscripted, narrative. A small-scale evaluation is discussed and the lessons learned are described.

Qualitative neuropsychological performance characteristics in frontotemporal dementia and Alzheimer's disease.

Abstract: Background: Frontotemporal dementia (FTD) and Alzheimer's disease are clinically distinct disorders, yet neuropsychological studies have had variable success in distinguishing them. A possible reason is that studies typically rely on overall accuracy scores, which may obscure differences in reasons for failure. Objectives: To explore the hypothesis that analysis of qualitative performance characteristics and error types, in addition to overall numerical scores, would enhance the neuropsychological distinction between FTD and Alzheimer's disease. Methods: 38 patients with FTD and 73 with Alzheimer's disease underwent assessment of language, visuospatial abilities, memory, and executive function, using a neuropsychological screening instrument and standard neuropsychological tests. In each of these cognitive domains, performance characteristics and error types were documented, in addition to numerical scores on tests. Results: Whereas comparison of neuropsychological test scores revealed some group differences, these did not occur consistently across tests within cognitive domains. However, analysis of performance characteristics and error types revealed qualitative differences between the two groups. In particular, FTD patients displayed features associated with frontal lobe dysfunction, such as concrete thought, perseveration, confabulation, and poor organisation, which disrupted performance across the range of neuropsychological tests. Conclusions: Numerical scores on neuropsychological tests alone are of limited value in differentiating FTD and Alzheimer's disease, but performance characteristics and error types enhance the distinction between the two disorders. FTD is associated with a profound behavioural syndrome that affects performance on cognitive assessment, obscuring group differences. Qualitative information should be included in neuropsychological research and clinical assessments.

Mold sensitization is common amongst patients with severe asthma requiring multiple hospital admissions.

Abstract: Background Multiple studies have linked fungal exposure to asthma, but the link to severe asthma is controversial. We studied the relationship between asthma severity and immediate type hypersensitivity to mold (fungal) and non-mold allergens in 181 asthmatic subjects.

Outcome uncertainty and the couch potato audience

Abstract: Previous studies of attendance demand for professional team sports have failed to yield clear- cut findings on the importance of outcome uncertainty to consumers. But potentially fewer problems should arise in examining the link between outcome uncertainty and demand in the television market for team sports, which in the case of English Premier League football is in fact a more important component in total club revenue. This study models both the choice of which games to show and the size of audience attracted by each game, exploiting data on audience sizes for games between 1993 and 2002. We propose a new measure of match outcome uncertainty and, from our results, both the broadcaster and the audience appear interested in competitive balance.

Learning by feeling: evoking empathy with synthetic characters

Abstract: Virtual environments (VEs) are now becoming a promising new technology to be used in the development of interactive learning environments for children. Perhaps triggered by the success of computer games, VEs are seen as an emergent and engaging new way by which children can learn experimental sciences and other disciplines. Inhabiting these IVEs can be agents or intelligent characters that are responsible for events that happen in the environment and make it not predictive or completely controlled. However, to build such environments, in particular, if populated by synthetic characters, one needs to carefully address the problem of how do the learners respond to the characters in the virtual environment. Do learners like the characters? Do learners identify themselves with characters in virtual environments? This relation between learners and characters in virtual environments can be studied in several perspectives. In this paper, we will focus primarily on the issue of empathy as one desirable a...

Uncovering the Limits of Patient-Centeredness: Implementing a Self-Management Trial for Chronic Illness:

Abstract: Research evaluating self-management of chronic conditions points to the effectiveness of interventions' changing the health behavior of individuals. However, we know little about how self-management is negotiated within health services. The authors designed a qualitative investigation to illuminate the quantitative findings of a randomized controlled trial (RCT) of a self-management program for people with inflammatory bowel disease. They conducted in-depth interviews with physicians and patients, and qualitative analysis illuminated the nature of doctor-patient encounters and possible reasons for lack of change in patient satisfaction with the consultation. The findings suggest that factors inhibiting effective patient-centered consultations include failure of physicians to incorporate expressed need relevant to people's self-management activities fully, interpretation of self-management as compliance with medical instructions, and the organization of outpatients' clinics. Giving attention to these barriers might maximize the opportunities for patient self-management of chronic illness based on a therapeutic alliance with health care professionals.

Echinococcosis in Tibetan populations, western Sichuan Province, China.

Abstract: We screened 3,199 people from Shiqu County, Sichuan Province, China, for abdominal echinococcosis (hydatid disease) by portable ultrasound combined with specific serodiagnostic tests. Both cystic echinococcosis (CE) (Echinococcus granulosus infection) and alveolar echinococcosis (AE) (E. multilocularis) were co-endemic in this area at the highest village prevalence values recorded anywhere in the world: 12.9% were infected with one or the other form (6.8% CE and 6.2% AE). Prevalences of both CE and AE were significantly higher in female than male patients and increased with the age of the person screened. Pastoral herdsmen were at highest risk for infection (prevalence 19.0%). Prevalence of CE varied in 5 townships from 0% to 12.1%, whereas AE prevalence ranged from 0% to 14.3%. Risk factors associated with both infections included the number of owned dogs, frequency of contact with dogs, and sources of drinking water.

Exercise referral: the public health panacea for physical activity promotion? A critical perspective of exercise referral schemes; their development and evaluation

Abstract: This review critically explores the development, impact and evaluation of exercise referral schemes (ERS) in the UK. A rapid expansion in the use of such ERSs has been recorded throughout leisure and primary care settings, but the evidence underpinning their implementation has been sparse and predominantly limited to randomized control trial (RCT) research design. Consequently, understanding of exercise referral as a ‘real world’ intervention has been limited. Considering the increasing importance being placed on evidence-based practice and clinical effectiveness, it is no longer sufficient for service providers of exercise referral to ignore the need to evaluate schemes. The guidelines on evaluation provided by the National Quality Assurance Framework for Exercise Referral are limited, hence practitioners are often unsure of the best measures to use when assessing effectiveness. Predominantly, exercise professionals focus on the collection of physiological data but tend to ignore relevant psychological a...

Main contractor experiences of partnering relationships on UK construction projects

Abstract: Partnering provides a major opportunity for improving project performance, whilst offering direct benefits to the whole of the supply chain. Many research studies reinforce this assertion although there is less critical analysis examining the nature of partnering in practice and whether the claims made for it are consistently justified. The experiences of commercial surveyors and managers within the UK construction industry have been gathered in a pilot study, drawing on the opinions of 48 commercial managers employed by a leading national contractor. The perceptions and experiences of partnering relationships are generally positive, although the early optimism at the beginning of such arrangements is seldom sustained throughout the project lifecycle. Attitudes to partnering are similar whether the relationship is upstream (client/main contractor) or downstream (main contractor/subcontractor). The growth in popularity of alternative procurement methods and statutory adjudication are both regarded as havin...

Risk factors in patients presenting as an emergency with colorectal cancer

Abstract: Of 905 patients with colorectal cancer admitted to a single district general hospital, 272 (30 per cent) were admitted as emergencies. Emergency patients had more advanced tumours (Dukes stage B and C 96 per cent versus 88 per cent of those admitted electively, P < 0.006), a shorter history (median 3 versus 11 weeks, P < 0.0001), were less likely to be fully ambulatory (44 versus 80 per cent, P < 0.0001) and more likely to have abdominal pain (74 versus 51 per cent, P < 0.001) and vomiting (40 versus 10 per cent, P < 0.0001). More emergency patients were given stomas (56 versus 35 per cent, P < 0.0001) and died in hospital (19 versus 8 per cent, P < 0.0001). Of those who survived to be discharged, patients admitted as an emergency spent longer in hospital (median stay 16 versus 13 days, P < 0.0001) and had a poorer overall 5-year survival rate (29 versus 39 per cent, P = 0.0001). Emergency patients were significantly older (median 74 versus 72 years, P = 0.04) and much more likely to be widowed (41 versus 27 per cent, P = 0.0002) than those admitted for elective surgery. If the personal and resource disaster of emergency colorectal cancer admission is to be reduced, screening strategies targeted by demographic characteristics require investigation.