University of Sannio

About: University of Sannio is a education organization based out in Benevento, Italy. It is known for research contribution in the topics: Gravitational wave & LIGO. The organization has 1278 authors who have published 6125 publications receiving 167577 citations. The organization is also known as: Università degli Studi del Sannio & Universita degli Studi del Sannio.

The Two Isoforms of the Na+/Ca2+ Exchanger, NCX1 and NCX3, Constitute Novel Additional Targets for the Prosurvival Action of Akt/Protein Kinase B Pathway

Abstract: The proteins NCX1, NCX2, and NCX3 expressed on the plasma membrane of neurons play a crucial role in ionic regulation because they are the major bidirectional system promoting the efflux and influx of Na+ and Ca2+ ions. Here, we demonstrate that NCX1 and NCX3 proteins are novel additional targets for the survival action of the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway. Indeed, the doxycycline-dependent overexpression of constitutively active Akt1 in tetracycline (Tet)-Off PC-12 positive mutants and the exposure of Tet-Off PC-12 wild type to nerve growth factor induced an up-regulation of NCX1 and NCX3 proteins. NCX1 up-regulation induced by Akt1 activation occurred at the transcriptional level because NCX1 mRNA increased, and it was counteracted by cAMP response element-binding protein 1 inhibition through small interfering RNA strategy. In contrast, Akt1-induced NCX3 up-regulation recognized a post-transcriptional mechanism occurring at the proteasome level because 1) NCX3 transcript did not increase and 2) the proteasome inhibitor N -benzyloxycarbonyl ( Z )-Leu-Leu-leucinal (MG-132) did not further enhance NCX3 protein levels in Akt1 active mutants as it would be expected if the ubiquitin-proteasome complex was not already blocked by Akt1 pathway. As expected, in PC-12 Tet-Off wild-type cells MG-132 enhanced NCX3 protein levels. This up-regulation produced an increased activity of NCX function. Furthermore, NCX1 and NCX3 up-regulation contributed to the survival action of Akt1 during chemical hypoxia because both the silencing of NCX1 or NCX3 and the pharmacological paninhibition of NCX isoforms reduced the prosurvival property of Akt1. Together, these results indicated that NCX1 and NCX3 represent novel additional molecular targets for the prosurvival action of PI3-K/Akt pathway.

In search of an integrated framework of business longevity

Abstract: Even if the domain of business longevity has been enriched by the multidisciplinary nature of approaches used to investigate the phenomenon, the lack of a unifying perspective has impeded systematic research and caused definitional ambiguity. The main aim of this special issue is to extend existing knowledge on business longevity by integrating theoretical and empirical studies that adopt different approaches and perspectives. This is essential in order to identify the key factors of long-term success and the effects of longevity on firm performance. The multifaceted nature of business longevity research is mirrored in the five articles included in this special issue, that offer different and interesting perspectives for the investigation of the domain.

On the Impact of Refactoring Operations on Code Quality Metrics

Abstract: Refactorings are behavior-preserving source code transformations. While tool support exists for (semi) automatically identifying refactoring solutions, applying or not a recommended refactoring is usually up to the software developers, who have to assess the impact that the transformation will have on their system. Evaluating the pros (e.g., the bad smell removal) and cons (e.g., side effects of the change) of a refactoring is far from trivial. We present RIPE (Refactoring Impact Prediction), a technique that estimates the impact of refactoring operations on source code quality metrics. RIPE supports 12 refactoring operations and 11 metrics and it can be used together with any refactoring recommendation tool. RIPE was used to estimate the impact on 8,103 metric values, for 504 refactorings from 15 open source systems. 38% of the estimates are correct, whereas the median deviation of the estimates from the actual values is 5% (with a 31% average).

Resveratrol Couples Apoptosis with Autophagy in UVB-Irradiated HaCaT Cells

Abstract: UVB radiation causes about 90% of non-melanoma skin cancers by damaging DNA either directly or indirectly by increasing levels of reactive oxygen species (ROS). Skin, chronically exposed to both endogenous and environmental pro-oxidant agents, contains a well-organised system of chemical and enzymatic antioxidants. However, increased or prolonged free radical action can overwhelm ROS defence mechanisms, contributing to the development of cutaneous diseases. Thus, new strategies for skin protection comprise the use of food antioxidants to counteract oxidative stress. Resveratrol, a phytoalexin from grape, has gained a great interest for its ability to influence several biological mechanisms like redox balance, cell proliferation, signal transduction pathways, immune and inflammatory response. Therefore, the potential of resveratrol to modify skin cell response to UVB exposure could turn out to be a useful option to protect skin from sunlight-induced degenerative diseases. To investigate into this matter, HaCaT cells, a largely used model for human skin keratinocytes, were treated with 25 or 100 µM resveratrol for 2 and 24 hours prior to UVB irradiation (10 to 100 mJ/cm2). Cell viability and molecular markers of proliferation, oxidative stress, apoptosis, and autophagy were analyzed. In HaCaT cells resveratrol pretreatment: reduces UVB-induced ROS formation, enhances the detrimental effect of UVB on HaCaT cell vitality, increases UVB-induced caspase 8, PARP cleavage, and induces autophagy. These findings suggest that resveratrol could exert photochemopreventive effects by enhancing UVB-induced apoptosis and by inducing autophagy, thus reducing the odds that damaged cells could escape programmed cell death and initiate malignant transformation.