Worcester Foundation for Biomedical Research

About: Worcester Foundation for Biomedical Research is a based out in . It is known for research contribution in the topics: Estrone & Estrogen. The organization has 2195 authors who have published 2646 publications receiving 115809 citations. The organization is also known as: Worcester Foundation for Experimental Biology.

Chemical Studies of Hamster Reproductive Pheromones

Abstract: Male golden hamsters respond to the vaginal discharge of females in a number of ways. The vaginal discharge attracts males, stimulates their sexual behavior, decreases their aggressive behavior, reduces their tendency to scent mark with the flank gland, and increases their plasma testosterone levels (cf, Johnston, 1977), It is not clear to what extent these are responses to different compounds in the discharge and to what extent they are different aspects of the response to a single compound or mixture. This is one of the questions which we are attempting to answer as we study the chemistry of the discharge. This paper is primarily concerned with our recent work on the substances in vaginal discharge which stimulate sexual behavior. However, since attraction is an important component of the sexual response, we first will review the results of earlier work on the attractant response.

Mast cell distribution in the uterus of cycling and pregnant hamsters

Abstract: In this study the distribution of mast cells in the cephalic, middle, or caudal portions of the uterine horns did not differ. In cross-sections, however, mast cells were most numerous in the myometrium, intermediate in the tunica vasculare of the endometrium and least in the mucosa. In the endometrium, the mast cells were most numerous in the antimesometrial, least in the mesometrial and intermediate in the lateral quadrants. In the myometrium, however, the relative numbers of mast cells between the quadrants varied according to the day in the estrous cycle. On days 1 (the day after estrus), 2, 3, and 4, (the day of proestrus) of the estrous cycle, there were respectively 42.7, 21.8, 22.5 and 10.7 mast cells in the endometrium per 12 μ sections. The differences in means between groups was highly significant (P < 0.001). In mated females on 67, 79, 91, 103, 115, and 151 hours after ovulation, there were respectively 9.4, 8.4, 4.7, 5.6, 2.1, and 2.2 mast cells in the endometrium per 12 μ cross-section. The differences in the means were not significant. The mean number of mast cells per 12 μ cross-sections of uteri of ovariectomized females was 20 ± 8. Subcutaneous injections of 0.3 gamma estrogen did not significantly alter the number of mast cells but 0.6 gamma depressed the mean count to 11 ± 7. It appeared from these studies that the uterine mast cell numbers were related to the cyclic phenomena of estrous, but that the numerical relationship to the time of nidation was not clear.

Differential display protocol with selected primers that preferentially isolates mRNAs of moderate- to low-abundance in a microscopic system.

Abstract: A modified reverse transcription polymerase chain reaction (RT-PCR)-based differential display procedure with selected primers (SPR) was developed to increase the bias toward isolating moderate- to...