Institution
Worcester Foundation for Biomedical Research
About: Worcester Foundation for Biomedical Research is a based out in . It is known for research contribution in the topics: Estrone & Estrogen. The organization has 2195 authors who have published 2646 publications receiving 115809 citations. The organization is also known as: Worcester Foundation for Experimental Biology.
Topics: Estrone, Estrogen, RNA, Sperm, Microtubule
Papers published on a yearly basis
Papers
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TL;DR: In vitro incubation of labeled immature core subparticles, enriched in P70, with a partially purified proteolytic factor fraction shows an enrichment in both P40–42 and P30, suggesting the factor is more trypsin than chymotrypsin-like.
37 citations
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TL;DR: A spermicidal factor was found in fresh human, bovine, rabbit, guinea pig, and rat sera that kills the spermatozoa of its own species and the sperms of other species.
Abstract: A spermicidal factor was found in fresh human, bovine, rabbit, guinea pig, and rat sera. It kills the spermatozoa of its own species (except in the case of human serum) and the sperms of other species. It was unstable, thermolabile, and of large molecular size. It was present in limited quantity in the fresh serum and could be used up by a definite number of spermatozoa. It could be destroyed by sodium citrate, by Seitz filtration, by trypsin, and by snake venom. This factor was not present in tissue extracts and various plasma protein fractions. The strength or concentration of this factor varies in different individuals and in different species. This factor has several characteristics similar to those of complement.
37 citations
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TL;DR: It was possible to demembrante and reactivate not only freshly collected testicular, cauda epididymal, and ejaculated ram sperm but also sperm that had been stored for several days at 0 degrees C and for several months at -196 degrees C in rete testis fluid or egg yolk citrate media.
Abstract: It was possible to demembrante and reactivate not only freshly collected testicular, cauda epididymal, and ejaculated ram sperm but also sperm that had been stored for several days at 0 degrees C and for several months at -196 degrees C in rete testis fluid or egg yolk citrate media. Sperm were usually washed free of seminal plasma before demembranation, but this was not essential for reactivation. Bovine serum albumin (1.0%) in the wash medium increased the survival of sperm, but more than 0.25% in the extraction medium decreased reactivation. A macro-molecular component of cauda epididymal fluid also inhibited the reactivation of testicular sperm. Triton X-100 concentrations between 0.01% and 1.00% in the extraction medium were satisfactory for demembranating the sperm. Rapid cooling (i.e., cold shock) mimicked the effect of detergent in making the sperm responsive to added ATP and demonstrated that damage to ram sperm in cold shock does not involve the axoneme. Ejaculated and cauda sperm were reactivated immediately on addition of ATP and activity persisted for up to 10 min. Testicular sperm, on the other hand, required about 4 min to become fully reactivated. The optimal ATP concentration for activation of sperm was 0.1-1.0 mM. Magnesium ions (0.1-1.0 mM) were important for reactivation, and testicular sperm required a higher magnesium concentration than did cauda or ejaculated sperm. Manganese ions were almost as effective as magnesium for reactivating cauda epididymal and ejaculated sperm. Cobalt and cadmium ions were much less active for cauda and ejaculated sperm and none of these ions were effective for testicular sperm. Fluoride (25-50 mM) inhibited reactivation. The presence of 50 microM cAMP in the extraction medium or preincubation of testicular sperm with theophylline or caffeine increased low levels of activation, but this was not evident with ejaculated or cauda sperm. We conclude that the motor apparatus is already functionally assembled in spermatozoa on leaving the testis, but some fine adjustment must take place during maturation in the epididymis.
37 citations
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TL;DR: In examining protein phosphorylation in intact cells incubated with 32Pi, it is evident that the 32P‐polypeptides of the plasma membrane are among the most highly labelled species in the cell, despite their minor contribution to overall protein content.
Abstract: The appreciation of protein phosphorylation as a ubiquitous mechanism for the post-translational control of protein function has drawn our attention to the phosphorylation of plasma membrane proteins. We have studied this phenomenon in the human erythrocyte and rat adipocyte, and have observed several features, common to the two systems, which may be of general significance. In examining protein phosphorylation in intact cells incubated with 32Pi, it is evident that the 32P-polypeptides of the plasma membrane are among the most highly labelled species in the cell, despite their minor contribution to overall protein content. The addition of epinephrine (to adipocytes) or cAMP1 (to erythrocytes) increases the phosphorylation of certain peptides, whereas others are unaffected. The protein kinases mediating these phosphorylations are present in the plasma membrane as isolated, and can be divided into two groups–-cAMP dependent and cAMP independent. These two classes of kinase differ markedly in their substrate specificity toward endogenous and exogenous polypeptide substrates. Two classes of protein kinases with similar properties can be detected in the cytoplasm. The relationship between the membrane-bound and cytoplasmic enzymes is uncertain.
The potential roles of the plasma membrane cAMP dependent protein kinases are evident from the diverse effects of cAMP on surface properties; however, the prevalence of plasma membrane proteins phosphorylated via cAMP independent pathways is striking. Thus, elucidation of the regulatory properties of the plasma membrane cAMP independent protein kinases may give new insight into the control of a variety of surface phenomena not mediated by cAMP.
37 citations
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TL;DR: A general theoretical approach utilizing tracer methodology has been presented for the quantitative study of homogeneous irreversible consecutive reaction sequences under conditions of first-order kinetics, finding only a relatively small fraction of the pregnenolone formation from cholesterol could be accounted by the enzymatic reaction sequences.
37 citations
Authors
Showing all 2195 results
Name | H-index | Papers | Citations |
---|---|---|---|
Robert A. Weinberg | 190 | 477 | 240903 |
Harvey F. Lodish | 165 | 782 | 101124 |
E. J. Corey | 136 | 1377 | 84110 |
Peter Palese | 132 | 526 | 57882 |
Sten Orrenius | 130 | 447 | 57445 |
Aldons J. Lusis | 127 | 673 | 73786 |
Michel Goedert | 125 | 337 | 64671 |
Frederic D. Bushman | 119 | 442 | 84206 |
Robert H. Singer | 113 | 391 | 41493 |
Joel F. Habener | 112 | 427 | 43774 |
Ryuzo Yanagimachi | 102 | 438 | 40651 |
Jaak Panksepp | 99 | 446 | 40748 |
Hagan Bayley | 97 | 344 | 33575 |
John H. Hartwig | 96 | 260 | 30336 |
Joseph Avruch | 94 | 191 | 40946 |