Showing papers in "European Journal of Cell Biology in 1998"

TL;DR: Data indicate that a phospholipase C beta2 isoform mediates a denatonium benzoate-induced second messenger response of taste sensory cells in the circumvallate papillae.

TL;DR: Evidence is provided that enhanced tyrosine phosphorylation of intercellular junction proteins in MDCK epithelia increases paracellular permeability and can also induce prominent reorganization of the junctional complex.

TL;DR: The results show that actin microfilaments are not needed for either the retrograde fusion of the Golgi complex with the endoplasmic reticulum promoted by brefeldin A or the anterograde reassembly after the removal of the drug, or the ER-Golgi transport of VSV-G glycoprotein.

TL;DR: The immortalized HaCaT cells reveal a generally high competence to realize an epidermal phenotype in a natural environment and appear therefore qualified for in vitro studies on structural and regulatory aspects of keratinocyte physiology and pathology.

TL;DR: Three new human syntaxin cDNAs, presumably originating from alternative splicing of the same transcript, are reported, indicating that this protein is conserved through evolution, and syntaxin-16 belongs to a new subgroup of syntaxins.

TL;DR: Data provide direct evidence that the rat mast cell chymase RMCP-II can, in the absence of other inflammatory mediators, increase epithelial permeability via an effect on the paracellular route.

TL;DR: A mechanistic role for gelsolin cleavage during SMC apoptosis is suggested, a process implicated in vessel development as well as stability of atherosclerotic plaque.

TL;DR: GFP-actin's fluorescence was used in long-term imaging studies aimed at documenting changes in the cytoskeleton of rat bladder NBT-II carcinoma cells during the 24-hour growth factor-mediated epithelia to mesenchyme transformation.

TL;DR: It is shown by confocal laser scanning microscopy that αB-crystallin and hsp25 become associated with sarcomeric structures directly after the heat shock, indicating a cytoskeletal protective function.

TL;DR: It is suggested that transcriptional shutdown precedes lamina reorganization and chromatin breakdown during lens fibre cell denucleation during bovine lens Denucleation.

TL;DR: Subcellular localizations and interactions of actin and two actin cytoskeleton-related proteins, Cdc8 tropomyosin and actin-related protein 3, Arp3, in the fission yeast Schizosaccharomyces pombe are investigated, using specific antibodies and by gene disruption to suggest that the F-actin cables may be involved in the formation of the medial ring.

TL;DR: Cx37 protein was more abundant in embryonic kidney and lung than in the corresponding adult tissues, and differential centrifugation of plasma membrane fractions in sucrose gradients found that Cx37-containing gap junctions in lung were much smaller than Cx32 and Cx26 aggregates from liver.

TL;DR: Mint 3 is ubiquitously expressed in all tissues, with lowest levels in brain and testis whereas mint 1 and 2 appear to be brain-specific, suggesting that mints form a diverse family of proteins with specialized neuronal and ubiquitous isoforms.

TL;DR: It is found that adrenoleukodystrophy and adrenoleUKody Strophy related genes have strikingly different expression in most mouse tissues and human cell lines analyzed, indicating that the proteins do not function as obligatory partners but might rather fulfill similar metabolic functions in different tissues.

TL;DR: This work reports the construction of a truncation mutant of ovine Cx50 which mimicks the cleavage in the intact lens and reveals how fibre cells in the core region remain connected despite the acidic environment caused by elevated lactate levels.

TL;DR: The results indicated that heparin and fucoidan can be used as tools to further investigate the cellular mechanisms regulating the proliferation and migration of human vascular cells.

TL;DR: Endocytic traffic is blocked in multivesicular carrier endosomes at and below 26 degrees C, and reduced temperature slows down transport in the recycling pathway, without a complete block.

TL;DR: It is shown that the principal binding site for Vps45p on intracellular membranes is provided by Tlg2p rather than Pep12p, and that Vps 45p is required for stable expression of TlG2p, suggesting that the role of VPS45p in protein traffic is more complex than has previously been assumed.

TL;DR: The described method for isolation and characterization of BCVs proved to be a valuable tool to characterize the vacuolar compartment occupied by Y. pseudotuberculosis, and has potential to be applied to other vacuole resident pathogens whose trafficking is thought to play a role in pathogenesis.

TL;DR: This hypothesis well explains the reason not only for the survival of the stress fibers at a particular oblique angle, but also for the reduced numbers of stress fibers found at the other angles on cyclic deforming fields.

TL;DR: It is demonstrated that the Sec1p-like protein Vps45p is required for the fusion of Golgi-derived vesicles with the prevacuolar compartment indicating that VPS45 functions before VPS27 in the vacuolar biogenesis pathway.

TL;DR: This work has isolated a cDNA clone of high sequence identity to and with the characteristic features of human vimentin from the zebrafish, which was produced and studied its assembly behaviour and found that assembly is best at the normal body temperature of various species.

TL;DR: The similarity between tau phosphorylation in dividing neuronal cells and Alzheimer degenerating neurons may indicate that aging neurons exposed to inappropriate signals respond by an attempt to activate their machinery for regeneration.

TL;DR: Results suggest that the presence of adequately sulphated molecules, in particular HSPG, is important for lactoferrin interaction and activity on the breast cancer cells MDA-MB-231.

TL;DR: A proteasome activator protein termed PA28 or 118 regulator, composed of two homologous subunits (α and β) and a separate but related protein termed Ki antigen or PA28γ have been characterized and it was found that both PA28α and PA28β antisera label the cytoplasm and the nucleoli.

TL;DR: The results suggest that PrPc expression is associated with regulation of cellular resistance to oxidative stress in skeletal muscle.

TL;DR: It is indicated that bax may function as a primary response gene and together with lowered bcl-2 level may determine the induction of apoptotic process in LU10 leukemic cells exposed to TGF-βl.

TL;DR: Cell surface exposure of phosphatidylserine during apoptosis serves recognition and removal of the dying cell by phagocytes and loss of phospholipid asymmetry and PS exposure is investigated by immunocytochemistry and related to morphological changes.

TL;DR: It is concluded that malaria parasites utilize acidic intracellular compartments to regulate their cytosolic free calcium concentration, and addition of nigericin to intact parasites in calcium free-medium resulted in a transient elevation in the parasite cytosol.

TL;DR: The powerful synchronous contractions of the uterus in labor depend on electrical coupling of myometrial smooth muscle cells by gap junctions, and multiple connexin expression may contribute to modulation of gap junction function in human myometrians in vivo.