Showing papers in "Experimental Hematology in 2007"

TL;DR: It is found that CD34+AC133+VEGFR-2+ cells are HPCs that do not yield EC progeny, and the biological mechanism for their correlation with cardiovascular disease needs to be reexamined.

TL;DR: The studies correlating the frequency of JAK2(V617F) mutant allele and clonality, as well as the presence of homozygous wild-type JAK1 erythropoietin-independent erythroid colonies, provide compelling evidence that the JAK3(V517F) is not the PV-initiating mutation.

TL;DR: B Probable signature miRNAs for erythropoiesis are identified and are predicted to target genes involved in cell development and differentiation.

TL;DR: The miRNAs with regulated expression in erythropoiesis; one appeared to be PV-specific, and their miRNA expression levels define early, intermediate, and late stages of erythroid differentiation.

TL;DR: A correlation exists between the presence of V617F JAK2 mutation and selected hemostatic activation variables and a significant increase in plasma hypercoagulation markers was found in ET patients.

TL;DR: Functional studies using gain of function and loss of function approaches showed that miR-451 is associated with erythroid maturation, and overall miRNA expression levels are increased upon erythyroid differentiation.

TL;DR: Existing data on the radiation-induced modulation of cytokine expression by skin cells are reviewed and the major cytokines in the response of skin cells to ionizing radiation include IL (interleukin)-1, IL-6, tumor necrosis factor (TNF)-alpha, transforming growth factor -beta, and the chemokines IL-8 and eotaxin are reviewed.

TL;DR: Although the majority of cytogenetic aberrations in B MSC were not clonal and differed from chromosomal markers in HC from the same individual, detection of typical chromosomal changes in BMSC suggests enhanced genetic susceptibility of these cells in MDS/AML.

TL;DR: It is demonstrated that aminated nanofibers are superior substrates for ex vivo HSPCs expansion, which was correlated with the enhanced HSPC adhesion to these aminated nano-spacers.

TL;DR: This review of low dose-induced whole-body effects, especially cancer, shows that system responses to acute or chronic low-level exposures evolve from damage to the basic molecular level, mainly to DNA of stem cells, and from adaptive responses that may occur in the whole body.

TL;DR: It is demonstrated that primitive subsets of HPC have higher affinity to human MSC and the essential role of specific junction proteins for stabilization of cell-cell contact is indicated by their significant higher expression.

TL;DR: In vivo and in vitro studies demonstrated that differentiation of Flk-1(+)MSCs is controlled by the microenvironment, and it was demonstrated that cytokines secreted by irradiated lung cells could inhibit differentiation of flk- 1(+) MSCs into epithelial cells in vitro.

TL;DR: Gen-array, proteomic, and cell-signaling studies have not only helped to identify in vivo mechanisms of action and drug resistance to novel agents, but also aided in the design of promising combination-therapy protocols.

TL;DR: This review will summarize the in vitro and in vivo studies examining UCB stem cells and their potential use for therapeutic application for nonhematopoietic tissue and cell regeneration.

TL;DR: The role of the microenvironment in the pathophysiology and progression of MDS has remained controversial, although there is evidence that stroma and matrix components, and their interactions with clonal cells, play an important role.

TL;DR: Oral fucoidan significantly amplified the CXCR4(+) HPC population and the ability to mobilize HPC using sulfated polysaccharides and mobilize more HPC with high levels of CX CR4 could be clinically valuable.

TL;DR: There was significant disease spectrum overlap between JAK2 V617F andJAK2 exon 12 mutations, and MPL gene mutations were not associated with erythrocytosis, but segregated primarily with the phenotypes of thrombocytosis and extramedullary disease, myelofibrosis, and osteosclerosis.

TL;DR: Understanding the basis of clonal selection and expansion will not only delineate further the pathophysiology of PNH but also provide new insights into stem cell biology and suggest novel therapeutic strategies for enhancing marrow function.

TL;DR: Normal tissues, including the central nervous system, tolerate single exposures to narrow planes of synchrotron-generated x-rays (microplanar beams; microbeams) up to several hundred Gy, and in both studies the repair processes could have involved "beneficial" bystander effects leading to tissue restoration.

TL;DR: Blood platelets have an immunoregulatory role that might be applied clinically in multitransfused patients (e.g., hematopoietic stem cell transplantation) and emphasize the potentially important role of platelets in the adaptive immune response.

TL;DR: A novel long-term leukemic stem/progenitor assay in which new drugs can be tested and in which genes can be overexpressed or downmodulated using a lentiviral approach is presented in order to obtain more insight into the process ofLeukemic transformation and self-renewal.

TL;DR: Although capable of incipient differentiation, cytokine secretion of hUCB-derived mononuclear cells envisages the potential of an indirect effect in vivo, providing the means for a therapeutic outcome mediated by secondary effects.

TL;DR: It is demonstrated that human Lin(-)/CD34(-)/ALDH(+) cells are capable of long-term repopulation and Hoechst exclusion ability does not seem to be the method of choice for the isolation of human hematopoietic stem cells.

TL;DR: Telomerase-positive B-CLL patients have spontaneously occurring cytotoxic hTERT-specific T cells that might be explored as a therapeutic vaccine in B- CLL.

TL;DR: The data show that MSC efficiently improve expansion of adult PBSC, together with decreasing their allostimulatory capacity, which should provide a clinically relevant method for optimizing PBSC ex-vivo expansion, in particular when poor grafts are obtained.

TL;DR: A defined miRNA profile distinguishes PMF granulocytes from those of normal subjects and, partially, also from PV or ET patients.

TL;DR: OP9 cells when cocultured with hESCs support simultaneous generation of CD34(+) primitive hematopoietic cells and CD73(+) MSCs from h ESCs, which had typical morphological and cell surface marker characteristics of adult bone marrow-derived MSC's and could be differentiated toward osteogenic, adipogenic, and chondrogenic cells in vitro.

TL;DR: The irradiation experiments suggest that the YFP(+) platelets are enriched for newly made cells because YFP has a shorter half-life than platelets, and raises the possibility that platelet activity decreases rapidly during physiological aging.

TL;DR: Zebularine is an effective inhibitor of p15INK4B methylation and cell growth in human AML in vitro and provides a strong rationale for evaluating its clinical utility in the treatment of myeloid malignancies.

TL;DR: Although excess nonrelapse mortality can not be ruled out, relapse rate was relatively low, resulting in improved outcome, and standard-risk patients with chemo-sensitive disease may also benefit from ibritumomab tiuxetan-BEAM and ASCT.