Showing papers in "Micron in 2012"

TL;DR: The results show that, independently of the cancer type, single cells are characterized by the lower Young's modulus, denoting higher deformability of cancerous cells, and proved the AFM capability in recognition of a single, mechanically altered cell, also in cases when morphological changes are not visible.

TL;DR: This review provides a brief overview about both transmission electron microscopy (TEM) and scanning electron microscope (SEM) techniques which have been employed to characterise nanoemulsions and selected examples of electron microscopic studies of nanoemulsion studies are presented.

TL;DR: Practical aspects of sample preparation, handling, data collection, 3D reconstruction, and structure analysis, and perspectives of biological microtomography as the convergence of sciences in X-ray optics, biology, and structural analysis are provided.

TL;DR: This study shows that malignant thyroid cells are 3- to 5-fold softer in comparison to primary normal thyroid cells depending on duration between cell seeding and AFM experiments, which reveals cultivation period as an important factor that influences cell mechanics and which must be considered when comparing cells.

TL;DR: The results indicate that the biophysical properties, including elastic modulus, of each layer of the human cornea are unique and may play a role in the maintenance of homeostasis as well as in the response to therapeutic agents and disease states.

TL;DR: AFM nanoindentation capabilities have provided an excellent improvement over conventional nanomechanical tools and by integration of topographical data from imaging, enabled the rapid extraction and presentation of mechanical data for biological samples.

TL;DR: Conditions to obtain in situ observations in the high-resolution transmission electron microscopy (HRTEM) mode with an image resolution of 0.10nm are examined and a discussion on the electron-gas interactions responsible for gas-induced resolution deterioration is given.

TL;DR: The methods, technologies and tools involved in the different computational stages behind structural studies by ET, from image acquisition to interpretation of tomograms are reviewed, including noise reduction, segmentation and analysis of subvolumes.

TL;DR: The beam deceleration method for scanning electron microscopes (SEM) also referred to as "retarding" was applied to back-scattered electron (BSE) imaging of the flat block face of a resin embedded biological specimen under low accelerating voltage and low beam current conditions, indicating the advantages of BD for block face imaging of biological materials such as cells and tissues under low-voltage and lowbeam current conditions.

TL;DR: A novel integrated index called Oral Malignancy Index (OMI) is proposed using the HOS, LBP, LTE features, to diagnose benign or malignant tissues using just one number to help the oral onco-pathologists to screen the subjects rapidly.

TL;DR: The first direct images of purified macromolecular protein complexes are presented using in situ liquid scanning transmission electron microscopy, establishing the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution.

TL;DR: Some of the opportunities that the environmental TEM (ETEM) offers when combined with other in situ techniques will be explored, directly in the microscope, by combining electron-based and photon-based techniques and phenomena.

TL;DR: This review collates the fine structural alterations of the human retina in aging and various pathological situations and their links to the disease pathogenesis, wherein most changes occur at the level of the retinal pigment epithelium -Bruch's membrane and the photoreceptor layer, causing visual problems to the sufferers.

TL;DR: It is demonstrated that SICM imaging, especially using an ARS/hopping mode, is a useful technique with unique capabilities for imaging the three-dimensional topography of a range of biological samples under physiologically relevant aqueous conditions.

TL;DR: In this paper, a series of diffraction patterns at atomic columns, obtained using the diffraction imaging method, exhibited higher intensities in their central regions (0-11mrad) for light elements and in their peripheral regions (11-22mrad), for heavy elements.

TL;DR: Recommendations of preferred methods and conditions for local-thickness measurement in the TEM are provided and it is suggested that a large collection semiangle should be used to reduce diffraction-contrast effects.

TL;DR: An overview of the stroboscopic ultrafast electron microscope and the nanosecond-time-resolved single-shot instrument is given and examples of materials science studies and the near-term instrumentation developments of this technique are presented.

TL;DR: The Fe average valence state as a function of lithiation (discharge) has been measured by EELS and results are compared with average Fe valence obtained from electrochemical data.

TL;DR: Results provide novel evidence that peritubular dentin lacks collagen fibrils, PTD contains an organic scaffold embedded with mineral and the PTD organic matrix is manly composed of glycosaminoglycans, whereas the lamina limitans is primarily made of proteoglycan protein cores.

TL;DR: The studies reported on this paper exploit the full potential of an high resolution quantitative microscopy such as the atomic force microscopy to investigate different aspect of the erythrocytes' life, death and interaction with the environment to investigate the relationship between structure and function in normal and pathological cells.

TL;DR: The objective of this study was to present a review of the composition and organization of the ECM of tendons and to highlight the importance of the anisotropic optical properties in the study of alterations in theECM.

TL;DR: This review focuses on recent developments offered by the rapid advances in AFM imaging and force spectroscopy with emphasizes on microbial research.

TL;DR: This critical review takes the reader on a journey through recent developments in, and applications of, correlative microscopy, examining its impact in biomedical research and in the field of plant science.

TL;DR: Charpy impact testing and fracture surface analysis of the hardened samples reveal the cooling rate induced microstructural distinctions.

TL;DR: The displacement of the gold nanoparticles over time was three orders of magnitude smaller than expected on the basis of Brownian Motion, implying that nanoscale objects of flexible structure or freely floating, including nanoparticles and biological objects, can be imaged with nanoscales resolution, as long as they are in close proximity to a solid support structure.

TL;DR: Some of the experimental needs and challenges for performing in situ nanoindentation TEM experiments on nanoparticles are discussed and both diffraction contrast and phase contrast are shown on silver nanoparticles with diameters below 50nm.

TL;DR: It is concluded that there are five types of cells in the hemolymph of B. glabrata and B. straminea, namely: blast-like cells, granulocytes, type I hyalinocyte, type II hyalinocytes and type III Hyalinocytes.

TL;DR: Results support the theory that virulence potential is multifactorial and that other factors not evaluated in this study could be involved in the CVV physiopathogeny.

TL;DR: Different growth mechanisms of Sn crystal during the lithiation and delithiation processes of SnO(2) nanowires revealed by in situ transmission electron microscopy are reported.

TL;DR: The antennal sensilla of P. favieri are investigated by SEM, TEM and focused ion beam (FIB/SEM) technology and contrary to the hypothesis that Paussus species lack a Johston's organ, a non-connective chordotonal organ composed of 9 groups of scolopidia has been found inside the pedicel.