Book ChapterDOI
5.542 – Organ Printing
TLDR
Current two-dimensional and three-dimensional strategies for assembling cells as well as the necessary support materials such as hydrogels, bioinks, and natural and synthetic polymers adopted for organ-printing research are discussed.Abstract:
Organ-printing techniques offer the potential to produce living 3D tissue constructs to repair or replace damaged or diseased human tissues and organs. Using these techniques, spatial variations along multiple axes with high geometric complexity can be obtained. The level of control offered by these technologies to develop printed tissues will allow tissue engineers to better study factors that modulate tissue formation and function, and provide a valuable tool to study the effect of anatomy on graft performance.
In this chapter, we discuss the history behind substrate patterning, and cell and organ printing, and the rationale for developing organ-printing techniques with respect to the limitations of current clinical tissue engineering strategies in effectively repairing damaged tissues. We discuss current two-dimensional and three-dimensional strategies for assembling cells as well as the necessary support materials such as hydrogels, bioinks, and natural and synthetic polymers adopted for organ-printing research. Furthermore, given the current state-of-the-art in organ-printing technologies, we discuss some of their limitations and provide recommendations for future developments in this rapidly growing field.read more
Citations
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Organ Printing: Tissue Spheroids as Building Blocks
Vladimir Mironov,Jing Zhang,Carmine Gentile,K Brakke,Thomas C. Trusk,Karoly Jakab,Gabor Forgacs,Vladimirs Kasjanovs,Richard P. Visconti,Roger R. Markwald +9 more
TL;DR: Organ printing can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks.
A review on stereolithography and its applications in biomedical engineering
TL;DR: Stereolithography is a solid freeform technique (SFF) that was introduced in the late 1980s as discussed by the authors, which has the highest fabrication accuracy and an increasing number of materials that can be processed is becoming available.
Tissue engineering of articular cartilage with biomimetic zones
TL;DR: A recent review as mentioned in this paper focuses on the zonal organization of native articular cartilage, strategies being used to develop such organization, the reorganization that occurs after culture or implantation, and future prospects for the tissue engineering of articular Cartilage with biomimetic zones.
Journal ArticleDOI
Assessing the Impact of Electrohydrodynamic Jetting on Encapsulated Cell Viability, Proliferation, and Ability to Self-Assemble in Three-Dimensional Structures
TL;DR: In this article, the authors investigate the impact of electrohydrodynamic jetting (EHDJ) encapsulation on viability, proliferation, and functionality of the encapsulated cells, which consists in applying a highvoltage electrical field between a target substrate and a jetting needle, which is fed with a suspension of cells in a polymeric solution undergoing a sol-gel transition upon contact with the target.
Book ChapterDOI
Tailoring Bioengineered Scaffolds for Regenerative Medicine
TL;DR: The proposed chapter is a qualitative review over the biomaterials, processes, and scaffold designs for tailored bioprinting, highlighting the importance of an extensively discussion on tailoring biomechanical properties for bioengineered scaffolds.
References
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
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Journal ArticleDOI
Porous scaffold design for tissue engineering
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Journal ArticleDOI
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
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