A human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial HMG-box protein both in vivo and in vitro.
TLDR
It is reported here that the nuclear gene for human mitochondrial transcription factor A can be stably expressed in yeast cells devoid of the yeast homolog protein, effectively substituting for the yeast protein.Abstract:
Human mitochondrial transcription factor A is a 25-kDa protein that binds immediately upstream of the two major mitochondrial promoters, thereby leading to correct and efficient initiation of transcription. Although the nature of yeast mitochondrial promoters is significantly different from that of human promoters, a potential functional homolog of the human transcriptional activator protein has been previously identified in yeast mitochondria. The importance of the yeast protein in yeast mitochondrial DNA function has been shown by inactivation of its nuclear gene (ABF2) in Saccharomyces cerevisiae cells resulting in loss of mitochondrial DNA. We report here that the nuclear gene for human mitochondrial transcription factor A can be stably expressed in yeast cells devoid of the yeast homolog protein. The human protein is imported efficiently into yeast mitochondria, is processed correctly, and rescues the loss-of-mitochondrial DNA phenotype in a yeast abf2 strain, thus functionally substituting for the yeast protein. Both human and yeast proteins affect yeast mitochondrial transcription initiation in vitro, suggesting that the two proteins may have a common role in this fundamental process.read more
Citations
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Journal ArticleDOI
Transcriptional Paradigms in Mammalian Mitochondrial Biogenesis and Function
TL;DR: These transcriptional paradigms provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that dictate cell- and tissue-specific energetic properties.
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Transcriptional regulatory circuits controlling mitochondrial biogenesis and function
TL;DR: This review summarizes the understanding of the transcriptional regulatory mechanisms involved in the biogenesis and energy metabolic function of mitochondria in higher organisms.
Journal ArticleDOI
Mitochondrial DNA maintenance in vertebrates.
TL;DR: Because features of a transcription-primed mechanism appear to be conserved in vertebrates, a general model for initiation of vertebrate heavy-strand DNA synthesis is proposed.
Journal ArticleDOI
Mitochondrial transcription factor A regulates mtDNA copy number in mammals
Mats I. Ekstrand,Maria Falkenberg,Anja Rantanen,Chan Bae Park,Martina Gaspari,Kjell Hultenby,Pierre Rustin,Claes M. Gustafsson,Nils-Göran Larsson +8 more
TL;DR: Genetic evidence for a novel role for TFAM in direct regulation of mt DNA copy number in mammals is provided and it is demonstrated that mtDNA copy number is directly proportional to the total TFAM protein levels also in mouse embryos.
Journal ArticleDOI
DNA Replication and Transcription in Mammalian Mitochondria
TL;DR: The mitochondrion was originally a free-living prokaryotic organism, which explains the presence of a compact mammalian mitochondrial DNA in contemporary mammalian cells, and the proposition that some genes required for DNA replication and transcription were acquired together from a phage early in the evolution of the eukaryotic cell.
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