A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti.
Karla Saavedra-Rodriguez,Ludmel Urdaneta-Marquez,Shavanthi Rajatileka,Shavanthi Rajatileka,M. Moulton,Adriana E. Flores,Ildefonso Fernández-Salas,Juan A. Bisset,Mario H. Rodriguez,Philip J. McCall,Philip J. McCall,Martin J. Donnelly,Hilary Ranson,Hilary Ranson,Janet Hemingway,William C. Black +15 more
TLDR
The tools and information presented provide a means for early detection and characterization of kdr that is critical to the development of strategies for resistance management and showed a high rate of recombination even though the two codons are only separated by a ~250 bp intron.Abstract:
Pyrethroids are commonly used as mosquito adulticides and evolution of resistance to these compounds is a major threat to public health. 'Knockdown resistance' to pyrethroids (kdr) is frequently caused by nonsynonymous mutations in the voltage-gated sodium channel transmembrane protein (para) that reduce pyrethroid binding. Early detection of kdr is critical to the development of resistance management strategies in mosquitoes including Aedes aegypti, the most prevalent vector of dengue and yellow fever viruses. Brengues et al. described seven novel mutations in hydrophobic segment 6 of domain II of para in Ae. aegypti. Assays on larvae from strains bearing these mutations indicated reduced nerve sensitivity to permethrin inhibition. Two of these occurred in codons Iso1011 and Val1016 in exons 20 and 21 respectively. A transition in the third position of Iso1011 encoded a Met1011 replacement and a transversion in the second position of Val1016 encoded a Gly1016 replacement. We have screened this same region in 1318 mosquitoes in 32 additional strains; 30 from throughout Latin America. While the Gly1016 allele was never detected in Latin America, we found two new mutations in these same codons. A transition in the first position of codon 1011 encodes a Val replacement while a transition in the first position of codon 1016 encodes an Iso replacement. We developed PCR assays for these four mutations that can be read either on an agarose gel or as a melting curve. Selection experiments, one with deltamethrin on a field strain from Santiago de Cuba and another with permethrin on a strain from Isla Mujeres, Mexico rapidly increased the frequency of the Iso1016 allele. Bioassays of F(3) offspring arising from permethrin susceptible Val1016 homozygous parents and permethrin resistant Iso1016 homozygous parents show that Iso1016 segregates as a recessive allele in conferring kdr. Analysis of segregation between alleles at the 1011 and 1016 codons in the F(3) showed a high rate of recombination even though the two codons are only separated by a ~250 bp intron. The tools and information presented provide a means for early detection and characterization of kdr that is critical to the development of strategies for resistance management.read more
Citations
More filters
Journal ArticleDOI
Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans.
Catherine L. Moyes,John Vontas,John Vontas,Ademir Jesus Martins,Lee Ching Ng,Sin Ying Koou,Isabelle Dusfour,Kamaraju Raghavendra,João Pinto,Vincent Corbel,Jean-Philippe David,David Weetman +11 more
TL;DR: The available evidence for the geographical distribution of insecticide resistance in Aedes aegytpi and Ae.
Journal ArticleDOI
Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti
John Vontas,E. Kioulos,Nena Pavlidi,Evangelia Morou,A. Della Torre,Hilary Ranson,Hilary Ranson +6 more
TL;DR: The levels of resistance in Aedes albopictus is relatively low at present compared to Ae.
Journal ArticleDOI
Role of cytochrome P450s in insecticide resistance: impact on the control of mosquito-borne diseases and use of insecticides on Earth
TL;DR: Recent technological advances are helping to build a functional profile of the P450 determinants of insecticide metabolic resistance in mosquitoes, which will enable the design of new insecticides with optimized efficacy in different environments.
Journal ArticleDOI
Impact of environment on mosquito response to pyrethroid insecticides: Facts, evidences and prospects
TL;DR: Current knowledge on pyrethroid resistance mechanisms in mosquitoes is updated and available data on the impact of the environment on mosquito response to pyrethroids, including key environmental factors, such as the presence of urban or agricultural pollutants and biotic interactions between mosquitoes and their microbiome are discussed, and research perspectives to fill in knowledge gaps are suggested.
Journal ArticleDOI
Pyrethroid resistance in Aedes aegypti and Aedes albopictus: Important mosquito vectors of human diseases
TL;DR: The status of pyrethroid resistance in A. aegypti and A. albopictus is reviewed, mechanisms of resistance, fitness costs associated with resistance alleles and suggestions for future research are presented.
References
More filters
Journal ArticleDOI
Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
Journal ArticleDOI
DnaSP, DNA polymorphism analyses by the coalescent and other methods.
TL;DR: The present version of DnaSP introduces several new modules and features which, among other options, allow handling big data sets and conducting a large number of coalescent-based tests by Monte Carlo computer simulations.
Journal ArticleDOI
Linkage disequilibrium in finite populations
William G. Hill,Alan Robertson +1 more
TL;DR: A theoretical investigation has been made of the influence of population size and recombination fraction on linkage disequilibrium between a pair of loci and it was found that the mean value ofr2 was determined almost entirely byN c and time, measured proportional toN.
Journal ArticleDOI
A Test of Neutral Molecular Evolution Based on Nucleotide Data
TL;DR: A conservative statistical test of the neutral theory of molecular evolution's prediction that regions of the genome that evolve at high rates, as revealed by interspecific DNA sequence comparisons, will also exhibit high levels of polymorphism within species is presented.
Journal ArticleDOI
Statistical properties of the number of recombination events in the history of a sample of dna sequences
TL;DR: It is found that RM is often much less than R, therefore, the number of recombination events may be greatly under-estimated in a parsimonious reconstruction of the history of a sample.