A new gadolinium-based MRI zinc sensor.
Ana Cristina Esqueda,Jorge A. López,Gabriel Andreu-de-Riquer,José C. Alvarado-Monzón,S. James Ratnakar,Angelo J. M. Lubag,A. Dean Sherry,Luis M. De León-Rodríguez +7 more
TLDR
The properties of a novel Gd(3+)-based MRI zinc sensor are reported and it is shown to have a relatively strong binding affinity for Zn(2+) (K(D) = 33.6 nM), similar to the affinity of the Zn (2+) ion with HSA alone.Abstract:
The properties of a novel Gd3+-based MRI zinc sensor are reported. Unlike previously reported Gd3+-based MRI contrast agents, this agent (GdL) differs in that the agent alone binds only weakly with human serum albumin (HSA), while the 1:2 GdL:Zn2+ ternary complex binds strongly to HSA resulting in a substantial, 3-fold increase in water proton relaxivity. The GdL complex is shown to have a relatively strong binding affinity for Zn2+ (KD = 33.6 nM), similar to the affinity of the Zn2+ ion with HSA alone. The agent detects as little as 30 μM Zn2+ in the presence of HSA by MRI in vitro, a value slightly more than the total Zn2+ concentration in blood (∼20 μM). This combination of binding affinity constants and the high relaxivity of the agent when bound to HSA suggests that this new agent may be useful for detection of free Zn2+ ions in vivo without disrupting other important biological processes involving Zn2+.read more
Citations
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