Journal ArticleDOI
A rapid, efficient method for isolating DNA from yeast.
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TLDR
A method is described for the purification of chromosomal and plasmid DNA from the yeast Saccharomyces cerevisiae that is rapid, gives 75% of theoretical yield, and produces DNA that can be cut with restriction endonucleases.About:
This article is published in Gene.The article was published on 1986-01-01. It has received 312 citations till now. The article focuses on the topics: Ethanol precipitation & Yeast.read more
Citations
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A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
Charles S. Hoffman,Fred Winston +1 more
TL;DR: To release plasmid DNA for the transformation of Escherichia coli, cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform.
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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
Robert H. Schiestl,R D Gietz +1 more
TL;DR: A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 × 105 transformant per microgram of vector DNA and to 1.5% transformants per viable cell.
Journal ArticleDOI
The localization of replication origins on ARS plasmids in S. cerevisiae.
TL;DR: In this article, two-dimensional agarose gel electrophoresis was used to identify the origin of the yeast autonomous replication sequence ARS1 in the Saccharomyces cerevisiae.
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A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
TL;DR: An important feature of this 3.8-kb molecular construct that makes it especially useful is that recombination between the flanking direct repeats occurs at a high frequency (10-4) in vegetatively grown cultures.
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A ras-like protein is required for a post-Golgi event in yeast secretion
Antti Salminen,Peter Novick +1 more
TL;DR: It is proposed that the SEC4 product is a GTP-binding protein that plays an essential role in controlling a late stage of the secretory pathway.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Construction and applications of a highly transmissible murine retrovirus shuttle vector.
TL;DR: A murine retrovirus shuttle vector system for the efficient introduction of selectable and nonselectable DNA sequences into mammalian cells and recovery of the inserted sequences as molecular clones is developed.
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Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
David Botstein,S C Falco,Sue E. Stewart,Miles B. Brennan,Stewart Scherer,Dan T. Stinchcomb,Kevin Struhl,Ronald W. Davis +7 more
TL;DR: A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described and has recently been certified at the HV2 level by the National Institutes of Health.
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Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast
TL;DR: The mutants were tested for loss of viability, change in morphology, increase in cell number, and ability to synthesize protein, ribonucleic acid (RNA), and deoxyribonuclear acid (DNA) after a shift from 23 to 36 C as mentioned in this paper.
Sterile host yeasts (shy): a eukaryotic system of biological containment for recombinant dna
David Botstein,S. Carl Falco,Sue E. Stewart,Miles B. Brennan,Stewart Scherer,Dan T. Stinchcomb,Kevin Struhl,Ronald W. Davis +7 more
TL;DR: A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described in this article, where the principle of containment is sterility: the haploid host strains all contain a mating type-non-specific sterile mutation.