Aberrant hypermethylation of miR-9 genes in gastric cancer.
Kuo-Wang Tsai,Yu-Lun Liao,Chew-Wun Wu,Ling-Yueh Hu,Sung-Chou Li,Wen-Ching Chan,Meng-Ru Ho,Chun-Hung Lai,Hsiao-Wei Kao,Wen-Liang Fang,Kuo Hung Huang,Wen-chang Lin +11 more
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TLDR
Methylation-mediated silencing of these three miR-9 genes can be reactivated in gastric cancer cells through 5-Aza-dC treatment and showed that it was significantly down-regulated in Gastric cancers compared with adjacent normal tissues.Abstract:
Carcinogenesis of the stomach involves multiple steps including genetic mutation or epigenetic alteration of tumor suppressor genes or oncogenes. Recently, tumor suppressive miRNAs have been shown to be deregulated by aberrant hypermethylation during gastric cancer progression. In this study, we demonstrate that three independent genetic loci encoding for miR-9 (miR-9-1, miR-9-2 and miR-9-3) are simultaneously modified by DNA methylation in gastric cancer cells. Methylation-mediated silencing of these three miR-9 genes can be reactivated in gastric cancer cells through 5-Aza-dC treatment. Subsequent analysis of the expression levels of miR-9 showed that it was significantly down-regulated in gastric cancers compared with adjacent normal tissues (P value < 0.005). A similar tendency toward a tumor-specific DNA methylation pattern was shown for miR-9-1, miR-9-2 and miR-9-3 in 72 primary human gastric cancer specimens. Ectopic expression of miR-9 inhibited cell proliferation, migration and invasion, suggesting its tumor suppressive potential in gastric cancer progression.read more
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microRNA Therapeutics in Cancer — An Emerging Concept
TL;DR: The roles of miRNAs in tumorigenesis, the miRNA-based therapeutic strategies currently being evaluated for use in cancer, and the advantages and current challenges to their use in the clinic are focused on.
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DNA methylation and microRNA dysregulation in cancer.
TL;DR: It appears that miRNA genes involved in regulating cancer‐related pathways are silenced in association with CpG island hypermethylation, and re‐expression of miRNAs and the replacement of tumor suppressive miRNAAs using miRNA mimics or expression vectors could be effective approaches to cancer therapy.
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Advances in molecular biomarkers for gastric cancer: miRNAs as emerging novel cancer markers.
TL;DR: The small noncoding microRNAs (miRNAs) have been suggested to be critical regulators in the oncogenesis pathways and to serve as useful clinical biomarkers, emerging as a new type of cancer biomarker with immeasurable clinical potential.
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Epigenetic alteration and microRNA dysregulation in cancer
TL;DR: Aberrant methylation of miRNA genes is a potentially useful biomarker for detecting cancer and predicting its outcome and unraveling the relationship between epigenetic alteration and miRNA dysregulation may lead to the discovery of new therapeutic targets.
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microRNA-9 Suppresses the Proliferation, Invasion and Metastasis of Gastric Cancer Cells through Targeting Cyclin D1 and Ets1
TL;DR: It is indicated that miR-9 suppresses the expression of cyclin D1 and Ets1 via the binding sites in their 3′-UTR, thus inhibiting the proliferation, invasion and metastasis of gastric cancer.
References
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miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis
Li Ma,Jennifer J. Young,Harsha K. Prabhala,Elizabeth Pan,Pieter Mestdagh,Daniel Muth,Julie Teruya-Feldstein,Ferenc Reinhardt,Tamer T. Onder,Scott Valastyan,Frank Westermann,Frank Speleman,Jo Vandesompele,Robert A. Weinberg +13 more
TL;DR: In this article, the authors uncover a regulatory and signalling pathway involving a metastasis-promoting miRNA that is predicted to directly target expression of the key metastasissuppressing protein E-cadherin.
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Marilena V. Iorio,Carlo M. Croce +1 more
TL;DR: Current knowledge about the involvement of microRNAs in cancer and their potential as diagnostic, prognostic, and therapeutic tools is reviewed.
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