Active and Passive Smoking and Lifestyle Determinants of 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine Levels in Human Leukocyte DNA
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The results seem to confirm that ETS exposure is capable of inducing some oxidative DNA damage in circulating leukocytes and that coffee and tea consumption might partially protect against smoking-induced oxidation damage.Abstract:
We investigated the effects of smoking and exposure to environmental tobacco smoke (ETS) on oxidative DNA damage by measuring 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) levels in DNA of leukocytes of healthy donors (30 smokers, 29 nonsmokers, and 28 ETS-exposed subjects). Nonsmokers had lower 8-oxodGuo levels compared with smokers (5.94 ± 0.87 × 10−6 and 19.85 ± 4.75 × 10−6 2-deoxyguanosine, respectively, means ± SE, P = 0.00007). Subjects exposed to ETS had higher mean value of 8-oxodGuo compared with nonsmokers (9.18 ± 1.53 × 10−6 2-deoxyguanosine, mean ± SE), nonsignificant by univariate analysis ( P = 0.074). Multiregression analysis indicated that the increase of 8-oxodGuo levels induced by ETS was significant ( P = 0.045) and that coffee and tea consumption reduced DNA oxidation ( P = 0.0053). Oxidative leukocyte DNA damage was positively correlated with plasma cotinine levels in ETS-exposed subjects ( r = 0.47, P < 0.01, n = 28) and was increased by age in nonsmokers and ETS-exposed subjects ( P = 0.049). The results seem to confirm that ETS exposure is capable of inducing some oxidative DNA damage in circulating leukocytes and that coffee and tea consumption might partially protect against smoking-induced oxidation damage. (Cancer Epidemiol Biomarkers Prev 2005;14(12):2975–7)read more
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References
More filters
Journal Article
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples.
TL;DR: The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight.
Journal ArticleDOI
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG.
TL;DR: DCMP and dAMP are incorporated selectively opposite 8-oxodG with transient inhibition of chain extension occurring 3' to the modified base, and the potentially mutagenic insertion of dAMP is targeted exclusively to the site of the lesion.
Journal ArticleDOI
Tobacco Smoke Carcinogens and Lung Cancer
TL;DR: By focusing in this review on several important carcinogens in tobacco smoke, the complexities in understanding tobacco-induced cancer can be reduced, and new approaches for lung cancer prevention can be envisioned.
Journal ArticleDOI
Reactions of oxyl radicals with DNA.
Anthony P. Breen,John A. Murphy +1 more
TL;DR: This review focuses on the damage caused to DNA by reactive oxygen-centred radicals, which arise either from the radiolysis of water by ionizing radiation, or from a purely chemical source.
Journal ArticleDOI
Oxidative damage to DNA in diabetes mellitus
Paresh Dandona,Kuldip Thusu,Stephen Cook,B.D. Snyder,J Makowski,D Armstrong,Thomas M. Nicotera +6 more
TL;DR: Diabetic patients showed greater oxidative damage to DNA, with increased generation of ROS, than controls, and such changes might contribute to accelerated aging and atherogenesis in diabetes and to the microangiopathic complications of the disease.