Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine
I. Ghidoni,Theodora Chlapanidas,M. Bucco,F. Crovato,Mario Marazzi,Daniele Vigo,Maria Luisa Torre,Massimo Faustini +7 more
TLDR
The SVF represents a rich source of mesenchymal cells potentially able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, and neuronal, epithelial and endothelial cells, which are ideal candidates for use in regenerative medicine, tissue engineering, including gene therapy and cell replacement cancer therapies.Abstract:
Cell encapsulation, a strategy whereby a pool of live cells is entrapped within a semipermeable membrane, represents an evolving branch of biotechnology and regenerative medicine. For example, over the last 20 years, male and female gametes and embryos have been encapsulated with or without somatic cells for different purposes, such as in vitro gametogenesis, embryo culture, cell preservation and semen controlled release. Beside that, cell encapsulation technology in alginate, which is a natural biodegradable polymer that mimics the extracellular matrix and supports both cell functions and metabolism, has been developed with the aim of obtaining three-dimensional (3D) cultures. In this context, adipose-derived stromal vascular fraction (SVF) has attracted more and more attention because of its enormous potential in tissue regeneration. In fact, the SVF represents a rich source of mesenchymal cells (ADSCs), potentially able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, and neuronal, epithelial and endothelial cells. These cells are ideal candidates for use in regenerative medicine, tissue engineering, including gene therapy and cell replacement cancer therapies. As long as technological resources are available for large-scale cell encapsulation intended for advanced therapies (gene therapy, somatic cell therapy and tissue engineering), the state-of-the-art in this field is reviewed in terms of scientific literature.read more
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Alginate derivatization: a review of chemistry, properties and applications.
Siddhesh N. Pawar,Kevin J. Edgar +1 more
TL;DR: Progress towards controlled synthesis of alginate derivatives, and the properties and applications of these derivatives are reviewed.
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Stem cells from adipose tissue allow challenging new concepts for regenerative medicine.
TL;DR: The harvesting and processing of clinically relevant quantities of adipose tissue-derived mesenchymal stem cells, triggering of these stem cells toward lineage-specific differentiation, seeding of the triggered stem cells on a bioresorbable scaffold, and implantation of the resulting tissue-engineered construct are addressed.
Journal ArticleDOI
A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting.
Ju Young Park,Jong-Cheol Choi,Jin-Hyung Shim,Jung-Seob Lee,HyoungJun Park,Sung Won Kim,Junsang Doh,Dong-Woo Cho +7 more
TL;DR: The behavior of chondrocytes and osteoblasts to hyaluronic acid (HA) and type I collagen (Col-1) hydrogels is examined and bioprinting-based approaches can be successfully applied for osteochondral tissue regeneration.
Journal ArticleDOI
Polymer-based microparticles in tissue engineering and regenerative medicine.
Mariana B. Oliveira,João F. Mano +1 more
TL;DR: This review focuses on polymeric microparticle processing techniques and overviews several examples and general concepts related to the use of these systems in TE and RE applications.
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Encapsulated human mesenchymal stem cells: a unique hypoimmunogenic platform for long-term cellular therapy.
TL;DR: Alginate‐PLL micro‐capsules are designed that can encapsulate human MSCs (hMSCs) for extended periods, as demonstrated by fluorescence and H3‐thymidine assays, and clearly demonstrate that hMSCs are the cell of choice for microencapsulation cell based therapy, thus bringing this technology closer to clinical application.
References
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Microencapsulated islets as bioartificial endocrine pancreas
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TL;DR: The microencapsulated islets remained morphologically and functionally intact throughout long-term culture studies lasting over 15 weeks.
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Linda G. Griffith,Gail Naughton +1 more
TL;DR: In the future, engineered tissues could reduce the need for organ replacement, and could greatly accelerate the development of new drugs that may cure patients, eliminating theneed for organ transplants altogether.
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Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells.
Gino Rigotti,Alessandra Marchi,Mirco Galiè,Guido Baroni,Donatella Benati,Mauro Krampera,Annalisa Pasini,Andrea Sbarbati +7 more
TL;DR: This surgical procedure is a low-invasive therapeutic approach for resolving the late side effects of radiotherapy, and treatment with lipoaspirate transplantation is potentially extended to other forms of microangiopathies.