Allergen- and bacterial antigen-specific T-cell clones established from atopic donors show a different profile of cytokine production.
Paola Parronchi,Donatella Macchia,Marie-Pierre Piccinni,Priscilla Biswas,C Simonelli,Enrico Maggi,Mario Ricci,Aftab A. Ansari,Sergio Romagnani +8 more
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It is demonstrated that most allergen-specific helper T cells in atopic individuals are able to produce high amounts of IL-4 (and IL-5), but no IFN-gamma, which may explain why allergens induce production of IgE antibodies and increase eosinophils.Abstract:
We have established a large panel of T-cell clones (TCCs) specific for Dermatophagoides pteronyssinus and Lolium perenne group I grass pollen allergens (total, 61) and for tetanus toxoid and protein purified derivative bacterial antigens (total, 38) from the peripheral blood of two atopic individuals and then analyzed their ability to produce interleukin 4 (IL-4), IL-5, and interferon gamma (IFN-gamma). Upon stimulation with phorbol 12-myristate 13-acetate plus anti-CD3 antibody, the great majority of TCCs specific for bacterial components was able to produce both IL-4 and IFN-gamma, whereas most D. pteronyssinus- and L. perenne group I-specific TCCs produced IL-4, but no, or limited, IFN-gamma. Moreover, the mean amounts of IL-4 and IFN-gamma released by allergen-specific TCCs were significantly higher and lower, respectively, than the mean amounts produced by TCCs specific for bacterial components. Under the same experimental conditions, virtually all allergen-specific TCCs, but only one-third of tested TCCs specific for bacterial components, expressed IL-5 RNA and secreted IL-5 in their supernatants. Eighteen TCCs (nine specific for allergens and nine specific for bacterial components) were also assessed for their ability to induce IgE synthesis by autologous B cells in response to stimulation with the specific antigen. Under these experimental conditions, all allergen-specific TCCs, but only one-third of TCCs specific for bacterial components that produced IL-4 but no, or little, IFN-gamma induced the synthesis of detectable amounts of IgE. The demonstration that most allergen-specific helper T cells in atopic individuals are able to produce high amounts of IL-4 (and IL-5), but no IFN-gamma, may explain why allergens induce production of IgE antibodies and increase eosinophils.read more
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Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TL;DR: Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.
Journal ArticleDOI
Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
Roberto Manetti,Paola Parronchi,Maria Grazia Giudizi,Marie-Pierre Piccinni,Enrico Maggi,Giorgio Trinchieri,Sergio Romagnani +6 more
TL;DR: IL-12 and CD16+ cells appear to have inhibitory effects on the development of IL-4-producing cells and to play an inductive role in promoting Th1-like responses.
Journal ArticleDOI
Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones.
Padmini Salgame,John S. Abrams,Carol Clayberger,Harris Goldstein,Jacinto Convit,Robert L. Modlin,Barry R. Bloom +6 more
TL;DR: Functional subsets of human T cells were delineated by analyzing patterns of lymphokines produced by clones from individuals with leprosy and by T cell clones of known function, and interleukin-4 was found to be necessary for suppression in vitro.
Journal ArticleDOI
Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity.
David B. Corry,Hans G. Folkesson,Martha L. Warnock,David J. Erle,Michael A. Matthay,Jeanine P. Wiener-Kronish,Richard M. Locksley +6 more
TL;DR: These data implicate IL-4 generated during the period of lymphocyte priming with antigen in establishing the cascade of responses required to generate airway hyperractivity to inhaled antigen and suggest a role for IL-5 or eosinophils.
Journal ArticleDOI
Indoor allergens and asthma : Report of the third international workshop
TL;DR: The Third International Workshop on Indoor Allergens and Asthma was designed to discuss recent progress in basic and clinical research in this area, to formulate recommendations for allergen-specific management of asthma, and to consider future research directions.
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