Journal ArticleDOI
Aloin promotes osteogenesis of bone-marrow-derived mesenchymal stem cells via the ERK1/2-dependent Runx2 signaling pathway.
Peng Li,Junchao Kong,Zhuming Chen,Shuai Huang,Guihe Lv,Bo Wei,Jinsong Wei,Kaipeng Jing,Juanhua Quan,Jiaqi Chu +9 more
TLDR
Aloin promotes osteogenic differentiation of BMSCs through activation of the ERK1/2-Runx2 signaling pathway and effectively attenuated Runx2 activation in these cells and also suppressed osteoblastic differentiation.Abstract:
Osteoporosis is characterized by low bone mass and the degeneration of bone structure, conditions which increase the risk of fracture. Aloin has been shown to affect bone metabolism, but its role in osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) remains unclear. The aim of our study was to determine whether aloin promotes the proliferation and osteogenic differentiation of BMSCs and, if so, whether it acts via activation of the ERK1/2-Runx2 signaling pathway. We found that the different concentrations of aloin tested had no obvious cytotoxic effects on the viability of BMSCs. Under osteogenic induction conditions, aloin increased cellular alkaline phosphatase activity, promoted BMSC mineralization, and increased osteogenic-related gene expression. In addition, treating the BMSCs with the signal transduction inhibitor PD98059 (ERK1/2) effectively attenuated Runx2 activation in these cells and also suppressed osteoblastic differentiation. Overall, our study demonstrates that aloin promotes osteogenic differentiation of BMSCs through activation of the ERK1/2-Runx2 signaling pathway.read more
Citations
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Regulation of Runx2 by post-translational modifications in osteoblast differentiation.
TL;DR: Runx2 serves as the best therapeutic model gene for studying bone and bone-related diseases and its activity at the post-translational levels by the virtue of phosphorylation, acetylation, and ubiquitination in controlling the bone homeostasis.
Journal ArticleDOI
Disproportionate Effect of Sub-Micron Topography on Osteoconductive Capability of Titanium
TL;DR: Sub-micro-rough titanium is reported, the first report on sub-micron topography on a titanium surface promoting osteoblast function with minimal osseointegration, which ameliorates the disadvantage of micro-rough Titanium by restoring cell attachment and proliferation.
Journal ArticleDOI
Antiglycation Activity and HT-29 Cellular Uptake of Aloe-Emodin, Aloin, and Aloe arborescens Leaf Extracts
TL;DR: The present data surely support the traditional use of A. arborescens leaf extracts against hyperglycemic conditions, while aloin and aloe-emodin as potential drugs need further study.
Journal ArticleDOI
Concentration-dependent cellular behavior and osteogenic differentiation effect induced in bone marrow mesenchymal stem cells treated with magnetic graphene oxide
Yi He,Yiming Li,Guanhui Chen,Changbo Wei,Xiliu Zhang,Binghui Zeng,Chen Yi,Chao Wang,Dongsheng Yu +8 more
TL;DR: It is found that MGO was not only biocompatible at low concentrations, but also could significantly accelerate osteogenic differentiation in BMSCs, which could provide new insight into bone regeneration in the future.
Journal ArticleDOI
Non-flavonoid polyphenols in osteoporosis: preclinical evidence.
Daniele Bellavia,Fabio Caradonna,Eufrosina Dimarco,Viviana Costa,Valeria Carina,Angela De Luca,Lavinia Raimondi,Milena Fini,Carla Gentile,Gianluca Giavaresi +9 more
TL;DR: In this paper, a systematic review of non-flavonoid polyphenolic compounds for the treatment of osteoporosis is presented, highlighting pathways implicated in the action of these molecules that act often epigenetically.
References
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Osteoporosis: now and the future
TL;DR: Novel treatment strategies have been developed that aim to inhibit excessive bone resorption and increase bone formation and the most promising novel treatments include denosumab, a monoclonal antibody for receptor activator of NF-κB ligand; odanacatib, a specific inhibitor of the osteoclast protease cathepsin K; and antibodies against the proteins sclerostin and dickkopf-1, two endogenous inhibitors of bone formation.
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Pathogenesis of osteoporosis: concepts, conflicts, and prospects
TL;DR: This review examines the fundamental pathogenetic mechanisms underlying osteoporosis, which include: (a) failure to achieve a skeleton of optimal strength during growth and development; (b) excessive bone resorption resulting in loss of bone mass and disruption of architecture; and (c) Failure to replace lost bone due to defects in bone formation.
Journal ArticleDOI
Role and regulation of RUNX2 in osteogenesis.
TL;DR: The regulation of RUNX2 by factors commonly used during osteogenic studies will be discussed and the complexity of its regulation and its interactions within the osteoblast differentiation pathway are often overlooked.
Journal Article
Aloe-emodin Is a New Type of Anticancer Agent with Selective Activity against Neuroectodermal Tumors
Teresa Pecere,M. V. Gazzola,C. Mucignat,Cristina Parolin,Francesca Dalla Vecchia,Andrea Cavaggioni,Giuseppe Basso,Alberto Diaspro,B. Salvato,Modesto Carli,Giorgio Palù +10 more
TL;DR: Aloe-emodin (AE), a hydroxyanthraquinone present in Aloe vera leaves, has a specific in vitro and in vivo antineuroectodermal tumor activity and might represent a conceptually new lead antitumor drug.
Journal ArticleDOI
Mechanical stress-mediated Runx2 activation is dependent on Ras/ERK1/2 MAPK signaling in osteoblasts.
TL;DR: Findings indicate that mechanical stress regulates Runx2 activation and favors osteoblast differentiation through the activation of MAPK signal transduction pathways and Ras/Raf‐dependent ERK1/2 activation, independent of p38 MAPK signaling.