AMP-activated protein kinase phosphorylation of endothelial NO synthase
Zhi-Ping Chen,Ken I. Mitchelhill,Belinda J. Michell,David Stapleton,Ignacio Rodríguez-Crespo,Lee A. Witters,David A. Power,Paul R. Ortiz de Montellano,Bruce E. Kemp +8 more
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TLDR
It is shown that AMPK co‐immunoprecipitates with cardiac endothelial NO synthase (eNOS) and phosphorylates Ser‐1177 in the presence of Ca2+‐calmodulin (CaM) to activate eNOS both in vitro and during ischaemia in rat hearts.About:
This article is published in FEBS Letters.The article was published on 1999-01-29 and is currently open access. It has received 855 citations till now. The article focuses on the topics: AMPK & AMP-activated protein kinase.read more
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AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism
TL;DR: Through signaling, metabolic, and gene expression effects, AMPK enhances insulin sensitivity and fosters a metabolic milieu that may reduce the risk for obesity and type 2 diabetes.
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Calcium, ATP, and ROS: a mitochondrial love-hate triangle
TL;DR: A "two-hit" hypothesis is developed, in which Ca(2+) plus another pathological stimulus can bring about mitochondrial dysfunction, and the delicate balance between the positive and negative effects of Ca( 2+) and the signaling events that perturb this balance is highlighted.
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Myocardial Substrate Metabolism in the Normal and Failing Heart
TL;DR: This review discusses the metabolic changes that occur in chronic heart failure, with emphasis on the mechanisms that regulate the changes in the expression of metabolic genes and the function of metabolic pathways and the consequences of these metabolic changes on cardiac function.
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AMPK in Health and Disease
TL;DR: This review looks at how AMPK integrates stress responses such as exercise as well as nutrient and hormonal signals to control food intake, energy expenditure, and substrate utilization at the whole body level and the possible role of AMPK in multiple common diseases.
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AMP-activated protein kinase—an energy sensor that regulates all aspects of cell function
TL;DR: Although best known for its effects on metabolism, AMPK has many other functions, including regulation of mitochondrial biogenesis and disposal, autophagy, cell polarity, and cell growth and proliferation.
References
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Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
David S. Bredt,Solomon H. Snyder +1 more
TL;DR: It is shown that nitric oxide synthetase activity requires calmodulin, and the native enzyme appears to be a monomer.
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme (endothelium-derived relaxing factor/arginine/cGMP)
David S. Bredt,Solomon H. Snyder +1 more
TL;DR: In this paper, it was shown that NO synthetase is a calmodulin-requiring enzyme, and showed that NO formation is accompanied by the stoichiometric conversion of arginine to citrulline.
Journal ArticleDOI
The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell?
TL;DR: AMP-activated protein kinase and SNF1-related protein kinases in higher plants are likely to be involved in the response of plant cells to environmental and/or nutritional stress.
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Nitric oxide synthases: which, where, how, and why?
Thomas Michel,Olivier Feron +1 more
TL;DR: eNOS, the last of the three mammalian NOS isoforms to be isolated, was originally purified and cloned from vascular endothelium, but has since been discovered in cardiac myocytes, blood platelets, brain (hippocampus), and elsewhere.
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Evidence for 5' AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport
TL;DR: Data suggest that AICAR and contraction stimulate glucose transport by a similar insulin-independent signaling mechanism and are consistent with the hypothesis that AMPK is involved in exercise-stimulated glucose uptake.