Journal ArticleDOI
Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector.
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The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid and comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions.Abstract:
The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions where lactose carrier protein comprises 1.4% of the cytoplasmic membrane protein.read more
Citations
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Journal ArticleDOI
Sequence of the lactose permease gene
TL;DR: The nucleotide sequence of the lacY gene coding for lactose permease (M protein) in Escherichia coli has been determined and is predicted to consist of 417 residues (71% nonpolar), resulting in a protein with a molecular weight of 46,504.
Journal ArticleDOI
Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
K B Heller,E C Lin,T H Wilson +2 more
TL;DR: The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.
Journal ArticleDOI
Lessons from lactose permease
Lan Guan,H. Ronald Kaback +1 more
TL;DR: An X-ray structure of the lactose permease of Escherichia coli (LacY) in an inward-facing conformation has been solved and a working model for the mechanism is presented that involves alternating access of both the sugar- and H-binding sites to either side of the membrane.
Journal ArticleDOI
Purification and reconstitution of functional lactose carrier from Escherichia coli.
TL;DR: Results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow and that the purified protein was identified as the product of the lac y gene.
Journal ArticleDOI
The kamikaze approach to membrane transport
TL;DR: Novel approaches that have been developed and applied to one membrane transport protein, the lactose permease from Escherichia coli, are now being used to study various other membrane proteins.
References
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Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Genetic regulatory mechanisms in the synthesis of proteins.
François Jacob,Jacques Monod +1 more
TL;DR: The synthesis of enzymes in bacteria follows a double genetic control, which appears to operate directly at the level of the synthesis by the gene of a shortlived intermediate, or messenger, which becomes associated with the ribosomes where protein synthesis takes place.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
TL;DR: Preliminary analysis of the phospholipid composition of the isolated fractions of Salmonella typhimurium showed significant quantitative differences in the relative distribution of the major glycerophosphatides.