An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires.
Toshiyuki Ueki,David J. F. Walker,Trevor L. Woodard,Kelly P. Nevin,Stephen S. Nonnenmann,Derek R. Lovley +5 more
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TLDR
A strain of Escherichia coli was designed to express e-PNs by introducing a plasmid that contained an inducible operon with E. coli genes for type IV pili biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be assembled into e-PTs.Abstract:
Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs) are a revolutionary electronic material. They offer novel options for electronic sensing applications and have the remarkable ability to harvest electrical energy from atmospheric humidity. However, technical constraints limit mass cultivation and genetic manipulation of G. sulfurreducens. Therefore, we designed a strain of Escherichia coli to express e-PNs by introducing a plasmid that contained an inducible operon with E. coli genes for type IV pili biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be assembled into e-PNs. The e-PNs expressed in E. coli and harvested with a simple filtration method had the same diameter (3 nm) and conductance as e-PNs expressed in G. sulfurreducens. These results, coupled with the robustness of E. coli for mass cultivation and the extensive E. coli toolbox for genetic manipulation, greatly expand the opportunities for large-scale fabrication of novel e-PNs.read more
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Fundamentals, Applications, and Future Directions of Bioelectrocatalysis.
Hui Chen,Olja Simoska,Koun Lim,Matteo Grattieri,Mengwei Yuan,Fangyuan Dong,Yoo Seok Lee,Kevin Beaver,Samali Weliwatte,Erin M. Gaffney,Shelley D. Minteer +10 more
TL;DR: This review seeks to systematically and comprehensively detail the fundamentals, analyze the existing problems, summarize the development status and applications, and look toward the future development directions of bioelectrocatalysis.
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Electromicrobiology: the ecophysiology of phylogenetically diverse electroactive microorganisms
TL;DR: A growing body of research emphasizes their broad phylogenetic diversity and shows that these microorganisms have key roles in multiple biogeochemical cycles, as well as the microbiome of the gut, anaerobic waste digesters and metal corrosion as mentioned in this paper.
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Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms.
TL;DR: Three engineering strategies for improving the EET ability of EAMs are described in detail, with an emphasis on the cross-disciplinary integration of systems biology and synthetic biology to build high-performance EAM systems.
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Recent advances in methanogenesis through direct interspecies electron transfer via conductive materials: A molecular microbiological perspective
Hyun Jin Kang,Sang Hoon Lee,Tae Guen Lim,Jeong Hoon Park,Boram Kim,Pierre Buffière,Hee Deung Park +6 more
TL;DR: In this article, a review of the direct interspecies electron transfer mechanisms and relevant microorganisms identified to date using molecular microbiological methods was critically reviewed, and important findings thus revealed were analyzed.
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The blind men and the filament: Understanding structures and functions of microbial nanowires.
TL;DR: Use of cryo-electron microscopy with multimodal functional imaging and a suite of electrical, biochemical, and physiological studies find that rather than pili, nanowires are composed of cytochromes OmcS and OmcZ that transport electrons via seamless stacking of hemes over micrometers.
References
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Tunable metallic-like conductivity in microbial nanowire networks
Nikhil S. Malvankar,Madeline Vargas,Madeline Vargas,Kelly P. Nevin,Ashley E. Franks,Ching Leang,Byoung-Chan Kim,Byoung-Chan Kim,Kengo Inoue,Kengo Inoue,Tünde Mester,Tünde Mester,Sean F. Covalla,Sean F. Covalla,Jessica P Johnson,Jessica P Johnson,Vincent M. Rotello,Mark T. Tuominen,Derek R. Lovley +18 more
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