Journal ArticleDOI
An essential G1 function for cyclin-like proteins in yeast
TLDR
Using strains where CLN1 was expressed conditionally, the essential function of ClN proteins was found to be limited to the G1 phase, consistent with the hypothesis that Cln proteins activate the Cdc28 protein kinase, shown to be essential for the G 1 to S phase transition in S. cerevisiae.About:
This article is published in Cell.The article was published on 1989-12-22. It has received 546 citations till now. The article focuses on the topics: Cyclin-dependent kinase 1 & Cyclin Gene.read more
Citations
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Journal ArticleDOI
G1 phase progression: Cycling on cue
TL;DR: Phosphorylation of ~40~‘~’ by Clnl,Cln2-Cdc28 might trigger its ubiquitin- mediated degradation, thereby enabling the Cln-regulated kinases to control S phase entry indirectly.
Journal ArticleDOI
PEST sequences and regulation by proteolysis
TL;DR: Recent experimental support for the hypothesis that polypeptide sequences enriched in proline, glutamic acid, serine, and threonine target proteins for rapid destruction is provided with a number of papers providing strong evidence that PEST regions serve as proteolytic signals.
Journal ArticleDOI
p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest
Vjekoslav Dulic,William K. Kaufmann,William K. Kaufmann,Sandra J. Wilson,Sandra J. Wilson,Thea D. Tisty,Thea D. Tisty,Emma Lees,J. Wade Harper,Stephen J. Elledge,Steven I. Reed +10 more
TL;DR: The data suggest a model in which ionizing radiation confers G1 arrest via the p53-mediated induction of a Cdk inhibitor protein, and it is found that both whole-cell lysates and inactive cyclin E-Cdk2 complexes prepared from irradiated cells contained an activity capable of inactivating cyclinE-C DK2 complexes.
Journal ArticleDOI
Cyclin A is required at two points in the human cell cycle.
TL;DR: It is found that DNA synthesis and entry into mitosis are inhibited in human cells microinjected with anti‐cyclin A antibodies at distinct times, suggesting that cyclin A defines novel control points of the human cell cycle.
Journal ArticleDOI
Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle
Hitoshl Matsushime,Martine F. Roussel,Martine F. Roussel,Richard A. Ashmun,Charles J. Sherr,Charles J. Sherr +5 more
TL;DR: The timing of p36CYL expression, its rapid turnover in the absence of CSF-1, and its phosphorylation and transient binding to a cdc2-related polypeptide suggest that CYL genes may function during S phase commitment.
References
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Rapid and efficient site-specific mutagenesis without phenotypic selection.
TL;DR: The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.
Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Book ChapterDOI
One-step gene disruption in yeast
TL;DR: The one-step gene disruption techniques described here are versatile in that a disruption can be made simply by the appropriate cloning experiment and the resultant chromosomal insertion is nonreverting and contains a genetically linked marker.
Journal ArticleDOI
Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis
TL;DR: The rapid degradation of injected alpha- and beta-casein as well as the inverse correlation of PEST regions with intracellular stability indicate that the presence of these regions can result in the rapid intrace cellular degradation of the proteins containing them.