Journal ArticleDOI
An integrated salinity-driven workflow for rapid lipid enhancement in green microalgae for biodiesel application
TLDR
In this article, a laboratory-based integrated approach was undertaken for improvement of lipid accumulation in green microalgae under sodium chloride (NaCl) stress, and the maximum biomass yields were obtained in the media with initial NaCl concentrations at 2 g L−1 (for Chlorella) and 0.05 g L −1 (For Chlorococcum) after 14 days of culture.Abstract:
A laboratory based integrated approach was undertaken for improvement of lipid accumulation in green microalgae under sodium chloride (NaCl) stress. The maximum biomass yields (1.22 ± 0.04 g L−1 in Chlorella and 1.31 ± 0.04 g L−1 in Chlorococcum) were obtained in the media with initial NaCl concentrations at 2 g L−1 (for Chlorella) and 0.05 g L−1 (for Chlorococcum) after 14 days of culture. Interestingly, maximum lipid accumulation obtained was 45.8 ± 0.4% w/v for Chlorella (on day eight) and 36.33 ± 0.56% w/v for Chlorococcum (on day ten) at an exposure of 5 g L−1 and 1.5 g L−1 NaCl concentrations, respectively. Fatty acid profiles of methyl esters for neutral and polar lipid fractions showed the presence of myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1) and linoleic (C18:2) acids as major fatty acids. Fluorescence intensities (% positive Nile red stained cells) of neutral and polar lipid fractions were also higher in NaCl stress conditions. A significant correlation between polar lipid and neutral lipid was found in both Chlorella (r2 = 0.979) and Chlorococcum (r2 = 0.972). Flow cytometric study demonstrated a positive fluorescence signal for neutral and polar lipid bodies related to Nile red fluorescence. Associated biochemical changes, like those in total chlorophyll, carotenoid, carbohydrate and protein contents, were determined under variable NaCl concentrations. The elemental compositions (CHNS) and CO2 fixation rate were assessed using high lipid yielding cells under optimum NaCl concentrations. Biodiesel fuel properties were obtained from high lipid yielding cells of the test microalgae and compared with Indian and international diesel standards. Finally large-scale cultivation of Chlorella ellipsoidea was performed in a hybrid photobioreactor (800 L capacity) and the highest biomass and lipid content obtained was 1.66 ± 0.09 g L−1 (at 8 days) and 54.73 ± 1.78% w/v (at 10 days) at 5 g L−1 NaCl respectively. This optimized NaCl concentration makes it an economical substitute for biodiesel production on a large scale.read more
Citations
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Analysis of direct and indirect quantification methods of CO2 fixation via microalgae cultivation in photobioreactors: A critical review
TL;DR: In this article, a critical review of the direct and indirect microalgae CO2 fixation quantification methods in photobioreactors (PBRs) is presented, where the main aim is to analyse, contrast and discuss the differences as well as inaccuracies.
Journal ArticleDOI
Treatment of highly saline RO concentrate using Scenedesmus quadricauda for enhanced removal of refractory organic matter
Sung Kyu Maeng,Waris Khan,Ji-Won Park,Ihnsup Han,Hee Sung Yang,Kyung Guen Song,Wook Jin Choi,Sungpyo Kim,Hyoungmin Woo,Hyun-Chul Kim +9 more
TL;DR: In this article, Scenedesmus quadricauda microalgae participate in the phycoremediation of a reverse osmosis (RO) concentrate at a high salinity (8000mgCl−L−L −1).
Journal ArticleDOI
Novel biocatalyst for optimal biodiesel production from diatoms
G. Saranya,T. V. Ramachandra +1 more
TL;DR: Spectroscopic analysis on fatty acids and Fatty Acid Methyl Esters derived from diatom exhibited similarities in specific functional groups between algal oil and biodiesel, exhibiting potential scope for large scale application of environment friendly biocatalysts to enhance the conversion performances of the transesterification process.
Journal ArticleDOI
Indole-3-acetic-acid-induced phenotypic plasticity in Desmodesmus algae.
TL;DR: Indole-3-acetic acid (IAA), the most common naturally occurring plant hormone, can exert stimulatory at low concentrations and inhibitory effects at high concentrations on the growth of the green alga Desmodesmus, and it is discovered that lipid droplets accumulated in algal cells grown at a high IAA concentration.
Journal ArticleDOI
Current strategies on algae-based biopolymer production and scale-up
TL;DR: In this article , a review summarizes the global scenario as well as the precise technique of algae-based polyhydroxyalkanoates extraction and bioplastic production and different techniques for valorisation of PHA production, its biodegradability and its commercial applications.
References
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Journal ArticleDOI
Lipid content of marine microalgae Chaetoceros muelleri Lemmermann (Bacillariophyceae) grown at different salinities
Mário U. G. Barros,Anderson Alan da Cruz Coelho,José William Alves da Silva,João Henrique Cavalcante Bezerra,Renato Teixeira Moreira,Wladimir Ronald Lobo Farias,Ricardo Lafaiete Moreira +6 more
TL;DR: The present study aimed to evaluate the growth and concentration of total lipids of the microalga C. muelleri Lemmermann cultivated at three different salinities (15, 25 and 35) and showed that the lipid yield showed higher than expected levels.
Journal ArticleDOI
An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications
TL;DR: A simple and high-throughput method for determining in situ intracellular neutral lipid accumulation in Chlorella ellipsoidea and Chlorococcum infusionum with flow cytometry and confocal microscopy was established by employing a lipophilic dye, Nile red, and revealed a high rate of accumulation of cytosolic neutral lipids when stained with Nile red and other organic solvents.
Journal ArticleDOI
FAME Production and Fatty Acid Profiles from Moist Chlorella sp. and Nannochloropsis oculata Biomass
Rui Carlos Medeiros Alves Sobrinho,Laércio Vauchinski,Renata Rodrigues de Moura,Ednei Gilberto Primel,Paulo Cesar Abreu,Marcelo Gonçalves Montes D'Oca +5 more
TL;DR: Results show that in the presence of 50 % w/w water relative to the biomass, the hydrolysis–esterification process results in higher FFA and FAME yields and similar profiles of the fatty esters from dry and moist biomass materials, respectively.