An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter
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TLDR
Results suggest that direct binding of MLTF to an upstream element activates transcription from the major late promoter.About:
This article is published in Cell.The article was published on 1985-12-01 and is currently open access. It has received 613 citations till now. The article focuses on the topics: Upstream activating sequence & Response element.read more
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Journal ArticleDOI
A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.
Marianne Frommer,Louise E. McDonald,Douglas Spencer Millar,Christina M. Collis,Fujiko Watt,Geoffrey W. Grigg,Peter L. Molloy,Cheryl L. Paul +7 more
TL;DR: A genomic sequencing method is reported that provides positive identification of 5-methylcytosine residues and yields strand-specific sequences of individual molecules in genomic DNA, which suggests that the high methylation level of single-copy sequences in sperm may be locally modulated by binding of protein factors in germ-line cells.
Journal ArticleDOI
Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
Ranjan Sen,David Baltimore +1 more
TL;DR: In this paper, an electrophoretic mobility shift assay with end-labeled DNA fragments was used to characterize proteins that bind to the immunoglobulin (Ig) heavy chain and the kappa light chain enhancers.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
PatentDOI
Max: a helix-loop-helix zipper protein that forms a sequence-specific dna-binding complex with myc and mad
TL;DR: In this paper, the Max polypeptide when associated with the Myc or Mad polyPEptide is capable of binding to nucleotide sequences containing CACGTG.
BookDOI
The Cell Cycle
TL;DR: It is shown that the presence of phosphatase inhibitors can have major effects on the levels of phosphorylation and the activities of the kinase extracted from cells, and the combination of okadaic acid and sodium vanadate was most effective in protecting p34cdc2 against cellular phosphatases.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
David J. Galas,Albert Schmitz +1 more
TL;DR: Estimates indicate that 10-fold sequence-specificity (differential binding constant) could be studied easily using this technique, and the binding of lac repressor to lac operator is visualized by "footprinting" as an example.
Journal ArticleDOI
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter
William S. Dynan,Robert Tjian +1 more
TL;DR: Results suggest that direct binding of Sp 1 to sequences in the upstream promoter element is the mechanism by which this factor activates transcription by RNA polymerase II at the SV40 early promoter.
Journal ArticleDOI
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
TL;DR: A cell-free system for studying the synthesis of mRNA in mammalian cells, which consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA.
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