Journal ArticleDOI
An ultrasensitive electrochemical biosensor based on multifunctional hemin/G-quadruplex nanowires simultaneously served as bienzyme and direct electron mediator for detection of lead ion
TLDR
In this paper, a direct electron transferred electrochemical biosensor with pseudo bienzyme cascade amplification for lead ion (Pb2+) detection was proposed, which showed enhanced selectivity to Pb2+ with a linear range from 10´fM to 200´nM and a low detection limit down to femtomolar level.Abstract:
In this work, the multifunctional hemin/G-quadruplex nanowires formed by rolling circle amplification (RCA) was used to develop a direct electron transferred electrochemical biosensor with pseudo bienzyme cascade amplification for ultrasensitive lead ion (Pb2+) detection. Target Pb2+ would specifically identify and cleave the Pb2+-dependent DNAzyme to facilitate the capture of primers that further triggered RCA for amounts of long hemin/G-quadruplex nanowires generation in the presence of hemin. With the introduction of nicotinamide adenine dinucleotide (NADH) in electrolyte, the formed hemin/G-quadruplex nanowires could synchronously act as NADH oxidase and horseradish peroxidase (HRP)-mimicking DNAzyme to accelerate direct electron transfer of hemin to the electrode surface. By using multifunctional hemin/G-quadruplex nanowires to simultaneously serve as bienzyme and direct electron mediator, the proposed electrochemical biosensor exhibited improved convenience, ultrasensitivity and stability without addition of other proteases and electrochemical signal tags. Under optimal conditions, this proposed electrochemical biosensor showed enhanced selectivity to Pb2+ with a linear range from 10 fM to 200 nM and a low detection limit down to femtomolar level, which provided a potential platform for Pb2+ analysis in real sample.read more
Citations
More filters
Journal ArticleDOI
Europium-Doped Lead-Free Cs3Bi2Br9 Perovskite Quantum Dots and Ultrasensitive Cu2+ Detection
Nan Ding,Donglei Zhou,Gencai Pan,Wen Xu,Xu Chen,Dongyu Li,Xiaohui Zhang,Jinyang Zhu,Yanan Ji,Hongwei Song +9 more
TL;DR: In this paper, an eco-friendly and nontoxic fluorescence probe for metal ion sensing was developed for detecting pollution triggered by highly toxic heavy metal ions, which has become of worldwide critical concern.
Journal ArticleDOI
An electrochemical aptasensor based on gold-modified MoS2/rGO nanocomposite and gold-palladium-modified Fe-MOFs for sensitive detection of lead ions
TL;DR: In this paper, an electrochemical aptasensor was proposed by using gold-modified molybdenum disulfide/reduced graphene oxide (Au@MoS2/rGO) nanocomposite and gold-palladium modified MIL-88 (Fe) metal-organic frameworks for sensitive detection of lead ions (Pb2+).
Journal ArticleDOI
G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection.
Hui Xi,Mario Juhas,Yang Zhang +2 more
TL;DR: This review highlights the latest developments in the field of G- quadruplex based biosensors, with particular focus on the G-quadruplex sequences and recent applications and the potential of G -quadru Plex based bios Sensors in SARS-CoV-2 detection.
Journal ArticleDOI
Electrochemical aptasensor based on gold modified graphene nanocomposite with different morphologies for ultrasensitive detection of Pb2
TL;DR: In this article, a sensitive electrochemical aptasensor was designed based on gold modified graphene nanocomposite with different morphologies for the detection of lead ion (Pb2+).
Journal ArticleDOI
Review of recent progress on DNA-based biosensors for Pb2+ detection
TL;DR: A review of Pb2+ recognition mechanisms from fundamental chemistry to the design of fluorescent, colorimetric, and electrochemical biosensors can be found in this article, where various signal amplification mechanisms such as rolling circle amplification, hairpin hybridization chain reaction and nuclease-assisted methods are coupled to these sensing methods to drive up sensitivity.
References
More filters
Journal ArticleDOI
Surface Plasmon Spectroscopy of Nanosized Metal Particles
TL;DR: In this paper, the use of optical measurements to monitor electrochemical changes on the surface of nanosized metal particles is discussed within the Drude model, and the absorption spectrum of a metal sol in water is shown to be strongly affected by cathodic or anodic polarization, chemisorption, metal adatom deposition, and alloying.
Journal ArticleDOI
What controls the melting properties of DNA-linked gold nanoparticle assemblies?
TL;DR: A series of experiments and a theoretical model designed to systematically define and evaluate the relative importance of nanoparticle, oligonucleotide, and environmental variables that contribute to the observed sharp melting transitions associated with DNA-linked nanoparticle structures are reported.
Journal ArticleDOI
A review on detection of heavy metal ions in water – An electrochemical approach
Manju Bhargavi Gumpu,Swaminathan Sethuraman,Uma Maheswari Krishnan,John Bosco Balaguru Rayappan +3 more
TL;DR: In this article, the toxicity mechanisms of various metal ions and their relationship towards the induction of oxidative stress have been summarized, and electrochemical biosensors employed in the detection of metal ions with various interfaces have been highlighted.
Journal ArticleDOI
Hemin-graphene hybrid nanosheets with intrinsic peroxidase-like activity for label-free colorimetric detection of single-nucleotide polymorphism.
TL;DR: This paper demonstrated for the first time a simple wet-chemical strategy for synthesizing hemin-graphene hybrid nanosheets (H-GNs) through the π-π interactions and developed a label-free colorimetric detection system for single-nucleotide polymorphisms (SNPs) in disease-associated DNA.
Journal ArticleDOI
Current progress in biosensors for heavy metal ions based on DNAzymes/DNA molecules functionalized nanostructures: A review
TL;DR: In this paper, the authors highlight the strategies for design of heavy metal biosensors benefiting from the use of DNAzymes/DNA molecules and nanomaterials and summarize the advances in biosensing devices/chip based on this method for the detection of metal ions.