Antimicrobial Resistance Gene Detection and Plasmid Typing Among Multidrug Resistant Enterococci Isolated from Freshwater Environment.
Sohyun Cho,John B. Barrett,Jonathan G. Frye,Charlene R. Jackson +3 more
- Vol. 8, Iss: 9, pp 1338
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TLDR
While AR genes commonly found in human and animals were detected in this study among environmental enterococci, resistance genes could not be determined for many of the isolates, which indicates that diverse AR mechanisms exist among enteritisci, and the understanding of AR mechanisms for environmentalEnterococci is limited.Abstract:
In this study, mechanisms of antimicrobial resistance (AR) as well as the abundance and diversity of plasmids were determined among multidrug resistant (MDR) enterococci from surface water in GA, USA. A total of 51 enterococci isolates were screened for the presence of 27 AR genes conferring resistance to ciprofloxacin, erythromycin, tylosin, kanamycin, streptomycin, lincomycin, Quinupristin/Dalfopristin (Q/D), and tetracycline. A plasmid classification system based on replication genes was used to detect 19 defined Gram-positive plasmid replicon families. Twelve genes were identified as conferring resistance to erythromycin and tylosin (erm(B) and erm(C)), kanamycin (aph(3')-IIIa), streptomycin (ant(6)-Ia), lincomycin (lnu(B)), Q/D (vat(E)), ciprofloxacin (qnrE. faecalis), and tetracycline (tet(K), tet(L), tet(M), tet(O) and tet(S)). Twelve different rep-families were identified in two-thirds of the isolates. While AR genes commonly found in human and animals were detected in this study among environmental enterococci, resistance genes could not be determined for many of the isolates, which indicates that diverse AR mechanisms exist among enterococci, and the understanding of AR mechanisms for environmental enterococci is limited. Diverse rep-families were identified among the enterococci recovered from the aquatic environment, and these rep-families appear to be quite different from those recovered from other sources. This work expands knowledge of AR gene reservoirs and enterococcal plasmids across a wider range of environments.read more
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Journal ArticleDOI
Characterization and Comparison of Enterococcus spp. Isolates from Feces of Healthy Dogs and Urine of Dogs with UTIs
Dagmara Stępień-Pyśniak,Fabrizio Bertelloni,Marta Dec,Giulia Cagnoli,Dorota Pietras-Ożga,Renata Urban-Chmiel,Valentina Virginia Ebani +6 more
TL;DR: In this paper, the authors analyzed and compared Enterococcus spp. isolated from healthy dog stools and sick dog urine and tested for antimicrobial resistance, biofilm production and presence of resistance and virulence genes.
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Occurrence of multidrug-resistant Enterococcus faecium isolated from environmental samples.
L.D.R. dos Santos,João Pedro Rueda Furlan,Inara Fernanda Lage Gallo,Micaela Santana Ramos,Eduardo Angelino Savazzi,Eliana Guedes Stehling +5 more
TL;DR: In this paper, a total 40 E. faecium isolates were recovered from 171 environmental samples and all of them were classified as multidrug resistant (MDR) isolates, highlighting the resistance to the fluoroquinolones class, linezolid and vancomycin.
Journal ArticleDOI
Antimicrobial Resistance, Biofilm Formation, and Virulence Genes in Enterococcus Species from Small Backyard Chicken Flocks
Othman Alzahrani,Mahmoud Fayez,Amal S. Alswat,Mohamed Alkafafy,Samy F. Mahmoud,Theeb M. Al-Marri,Ahmed Almuslem,Hassan Ashfaq,Shaymaa Yusuf +8 more
TL;DR: The results show that healthy backyard chickens could act as a reservoir for MDR and virulent Enterococcus spp.
Journal ArticleDOI
Non-point source fecal contamination from aging wastewater infrastructure is a primary driver of antibiotic resistance in surface waters.
Julian Damashek,Jason R. Westrich,Jacob M. McDonald,Morgan E. Teachey,Charlene R. Jackson,Jonathan G. Frye,Erin K. Lipp,Krista A. Capps,Elizabeth A. Ottesen +8 more
TL;DR: In this paper , the authors quantified fecal marker genes and antibiotic resistant gene (ARG) transfer in 992 stream water samples collected seasonally during a 5-year period from 115 sites across the Upper Oconee watershed (Georgia, USA).
Journal ArticleDOI
Vancomycin Resistance in Enterococcus and Staphylococcus aureus
TL;DR: In this article , the authors discuss the epidemiology of vancomycin-resistant Enterococcus and S. aureus in healthcare, community, and agricultural settings, and explore van-resistant resistance in the context of van and non-van mediated resistance development.
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