B Cell Epitopes of Four Fimbriae Antigens of Klebsiella pneumoniae: A Comprehensive In Silico Study for Vaccine Development.
Fatemeh Nemati Zargaran,Alisha Akya,Shahab Rezaeian,Keyghobad Ghadiri,Roya Chegene Lorestani,Hamid Madanchi,Hamid Madanchi,Sadegh Safaei,Mosayeb Rostamian +8 more
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TLDR
Four B cell epitopes of K. pneumoniae Fim antigens are found that are immunogen, antigenic, not similar to human peptides, not allergen and not toxic, and suitable physiochemical properties to administrate as vaccine, although their complete efficacy should be also shown in vitro and in vivo.Abstract:
Klebsiella pneumoniae is one of the major causes of nosocomial infections worldwide which can cause several diseases in children and adults. The globally dissemination of hyper-virulent strains of K. pneumoniae and the emergence of antibiotics-resistant isolates of this pathogen narrows down the treatment options and has renewed interest in its vaccines. Vaccine candidates of Klebsiella pneumoniae have not been adequately protective, safe and globally available yet. In K. pneumoniae infection, it is well known that B cells that induce robust humoral immunity are necessary for the host complete protection. Identifying the B cell epitopes of antigens is valuable to design novel vaccine candidates. In the present study using immunoinformatics approaches we found B cell epitopes of four K. pneumoniae type 1 fimbriae antigens namely FimA, FimF, FimG, and FimH. Linear and conformational B cell epitopes of each antigen were predicted using different programs. Subsequently, many bioinformatics assays were applied to choose the best epitopes including prediction antigenicity, toxicity, human similarity and investigation on experimental records. These assays resulted in final four epitopes (each for one Fim protein). These final epitopes were modeled and their physiochemical properties were estimated to be used as potential vaccine candidates. Altogether, we found four B cell epitopes of K. pneumoniae Fim antigens that are immunogen, antigenic, not similar to human peptides, not allergen and not toxic. Also, they have suitable physiochemical properties to administrate as vaccine, although their complete efficacy should be also shown in vitro and in vivo.read more
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Immunoinformatics and molecular docking studies reveal a novel Multi-Epitope peptide vaccine against pneumonia infection.
Soumya Ranjan Mahapatra,Jyotirmayee Dey,Taranjeet Kaur,Rajlaxmi Sarangi,Atul Anand Bajoria,Gajraj Singh Kushwaha,Namrata Misra,Mrutyunjay Suar +7 more
TL;DR: In this paper, a variety of immunoinformatics techniques was utilized to develop a novel multi-epitope vaccine that targets the highly immunodominant type 3 fimbrial protein of Klebsiella pneumoniae, the causal organism for pneumonia.
Journal ArticleDOI
Prophylaxis and Treatment against Klebsiella pneumoniae: Current Insights on This Emerging Anti-Microbial Resistant Global Threat
Vanessa Arato,Maria Michelina Raso,Gianmarco Gasperini,Francesco Berlanda Scorza,Francesca Micoli +4 more
TL;DR: A review of the current status of the epidemiology and the molecular pathogenesis of Klebsiella pneumoniae and explore specific bacterial antigens as potential targets for both prophylactic and therapeutic solutions is presented in this paper.
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Harnessing the Potential of Multiomics Studies for Precision Medicine in Infectious Disease.
Rebecca A Ward,Nima Aghaeepour,Roby P. Bhattacharyya,Roby P. Bhattacharyya,Clary B. Clish,Brice Gaudilliere,Nir Hacohen,Nir Hacohen,Michael K. Mansour,Philip A. Mudd,Shravani Pasupneti,Shravani Pasupneti,Rachel M. Presti,Eugene P. Rhee,Pritha Sen,Andrej Spec,Jenny M. Tam,Jenny M. Tam,Alexandra-Chloé Villani,Alexandra-Chloé Villani,Ann E. Woolley,Joe L. Hsu,Joe L. Hsu,Jatin M. Vyas +23 more
TL;DR: In this paper, the authors proposed a system approach to define the true risk of future infection for a patient, and integrated patient-specific data using machine learning to improve the effectiveness of prediction.
Journal ArticleDOI
Detecting the Dominant T and B Epitopes of Klebsiella pneumoniae Ferric Enterobactin Protein (FepA) and Introducing a Single Epitopic Peptide as Vaccine Candidate
TL;DR: In this article, the T and B cell-specific epitopes of K. pneumoniae FepA were identified using comprehensive in-silico approaches and an antigenic peptide was introduced for vaccine development.
Journal ArticleDOI
Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
Silvia da Silva Fontes,Fernanda de Moraes Maia,Laura Santa’Anna Ataides,Fernando de Paiva Conte,Josué da Costa Lima-Junior,Tatiana Rozental,Matheus Ribeiro da Silva Assis,Adonai Alvino Pessoa Júnior,Jorlan Fernandes,Elba Regina Sampaio de Lemos,Rodrigo Nunes Rodrigues-da-Silva +10 more
TL;DR: In this article, the authors used immunoinformatics and computational biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins (OMP-H, Com-1, and OMP-P1).
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