Journal ArticleDOI
β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production
Sang-Mok Lee,Li Hua Jin,Jae Hyun Kim,Sung Ok Han,Hyon Bin Na,Taeghwan Hyeon,Yoon Mo Koo,Jungbae Kim,Jung heon Lee +8 more
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TLDR
It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.Abstract:
β-Glucosidase (βG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.read more
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Journal ArticleDOI
Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization
Oveimar Barbosa,Claudia Ortiz,Ángel Berenguer-Murcia,Rodrigo Torres,Rafael C. Rodrigues,Roberto Fernandez-Lafuente +5 more
TL;DR: Glutaraldehyde, an apparently old fashioned reactive, remains the most widely used and with broadest application possibilities among the compounds used for the design of biocatalyst.
Journal ArticleDOI
Perspective of Recent Progress in Immobilization of Enzymes
Daniel N. Tran,Kenneth J. Balkus +1 more
TL;DR: Recent work regarding the immobilization of enzymes in inorganic mesoporous materials as well as the modifications to those materials is summarized in this paper.
Journal ArticleDOI
Enzyme stabilization by nano/microsized hybrid materials
TL;DR: This review discusses different approaches to improve enzyme stability in various materials such as nanoparticles, nanofibers, mesoporous materials, sol–gel silica, and alginate‐based microspheres to be environmental friendly, inexpensive, and easy to use for enzyme‐based industrial applications.
Journal ArticleDOI
From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.
TL;DR: This review highlights and summarizes various studies that have aimed to improve the biochemical properties of industrially significant enzymes.
Journal ArticleDOI
Application of nanoparticles in biofuels: an overview
Patrick T. Sekoai,Cecil N.M. Ouma,Stephanus P. du Preez,Phillimon Modisha,Nicolaas Engelbrecht,Dmitri Bessarabov,Anish Ghimire +6 more
TL;DR: This review critically examines the various studies in literature that have explored nanoparticles in biofuel processes such as biohydrogen, biogas, biodiesel and bioethanol production, towards enhancing their process yields and elucidates the different types of nanomaterials that have been used in these bioprocesses.
References
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Journal ArticleDOI
BIOMASS ETHANOL: Technical Progress, Opportunities, and Commercial Challenges
TL;DR: In this article, the authors discuss the benefits of cellulose and hemicellulose fractions and the major challenges to realizing the great benefits of biomass ethanol without subsidies, and propose to use genetically engineered bacteria to ferment all five sugars in biomass to ethanol.
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Journal ArticleDOI
Nanobiocatalysis and Its Potential Applications
TL;DR: Recent developments in nanobiocatalysis are described and their potential applications in various fields, such as trypsin digestion in proteomic analysis, antifouling, and biofuel cells are described.
Book ChapterDOI
Effects of Sugar Inhibition on Cellulases and β-Glucosidase During Enzymatic Hydrolysis of Softwood Substrates
TL;DR: This study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.