Bacillus cereus G9241 S-Layer Assembly Contributes to the Pathogenesis of Anthrax-Like Disease in Mice
Ya-Ting Wang,So-Young Oh,So-Young Oh,Antoni P. A. Hendrickx,Antoni P. A. Hendrickx,J. M. Lunderberg,J. M. Lunderberg,Olaf Schneewind,Olaf Schneewind +8 more
TLDR
B. cereus G9241 csaB mutant assembled capsular polysaccharides but displayed an increase in chain length relative to the wild-type strain, likely due to its inability to deposit BslO murein hydrolase at divisional septa.Abstract:
Bacillus cereus G9241, the causative agent of anthrax-like disease, harbors virulence plasmids encoding anthrax toxins as well as hyaluronic acid (HA) and B. cereus exopolysaccharide (BPS) capsules. B. cereus G9241 also harbors S-layer genes, including homologs of Bacillus anthracis surface array protein (Sap), extractable antigen 1 (EA1), and the S-layer-associated proteins (BSLs). In B. anthracis, S-layer proteins and BSLs attach via their S-layer homology domains (SLH) to the secondary cell wall polysaccharide (SCWP) in a manner requiring csaB, a predicted ketalpyruvate transferase. Here we used a genetic approach to analyze B. cereus G9241 S-layer assembly and function. Variants lacking the csaB gene synthesized SCWP but failed to retain Sap, EA1, and BSLs in the bacterial envelope. The B. cereus G9241 csaB mutant assembled capsular polysaccharides but displayed an increase in chain length relative to the wild-type strain. This phenotype is likely due to its inability to deposit BslO murein hydrolase at divisional septa. During growth under capsule-inducing conditions, B. cereus G9241 assembled BSLs (BslA and BslO) and the Sap S-layer protein, but not EA1, in the envelope. Finally, csaB-mediated assembly of S-layer proteins and BSLs in B. cereus G9241 contributes to the pathogenesis of anthrax-like disease in mice.read more
Citations
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Journal ArticleDOI
S-layers: principles and applications
TL;DR: Monomolecular arrays of protein or glycoprotein subunits forming surface layers (S‐layers) are one of the most commonly observed prokaryotic cell envelope components and revealed considerable application potential in (nano)biotechnology, biomimetics, biomedicine and synthetic biology.
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Biogenesis and functions of bacterial S-layers
TL;DR: The outer surface of many archaea and bacteria is coated with a proteinaceous surface layer (known as an S-layer), which is formed by the self-assembly of monomeric proteins into a regularly spaced, two-dimensional array.
Journal ArticleDOI
You Can't B. cereus - A Review of Bacillus cereus Strains That Cause Anthrax-Like Disease.
TL;DR: The emergence of these strains has reignited the debate surrounding classification of the B. cereus sensu lato group and serves as a reminder that the field of medical microbiology is constantly changing and remains an important and ongoing area of research.
Journal ArticleDOI
Assembly and Function of the Bacillus anthracis S-Layer.
TL;DR: Recent insights into the assembly and function of the S-layer and the SCWP are reviewed, which enable the pathogenesis of anthrax-like diseases.
Journal ArticleDOI
LytR-CpsA-Psr Enzymes as Determinants of Bacillus anthracis Secondary Cell Wall Polysaccharide Assembly
Megan Liszewski Zilla,Yvonne G. Y. Chan,J. M. Lunderberg,Olaf Schneewind,Dominique Missiakas,Dominique Missiakas +5 more
TL;DR: A model whereby B. anthracis LCPs promote attachment of SCWP precursors to discrete locations in the peptidoglycan, enabling BSL assembly and regulated separation of septal peptidlycercan is proposed.
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