Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis
Natalia Ivanova,Alexei Sorokin,Iain Anderson,Nathalie Galleron,Benjamin Candelon,Vinayak Kapatral,Anamitra Bhattacharyya,Gary Reznik,Natalia Mikhailova,Alla Lapidus,Lien Chu,Michael Mazur,Eugene Goltsman,Niels Bent Larsen,Mark D'Souza,Theresa L. Walunas,Yuri Grechkin,Gordon D. Pusch,Robert Haselkorn,Michael Fonstein,S. Dusko Ehrlich,Ross Overbeek,Nikos C. Kyrpides +22 more
TLDR
The sequencing and analysis of the type strain B. cereus ATCC 14579 together with the gapped genome of B. anthracis A2012 enables the comparative analysis to clarify the phylogeny of the cereus group, and the latter to determine plasmid-independent species-specific markers.Abstract:
Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndromes1. It is closely related to the animal and human pathogen Bacillus anthracis and the insect pathogen Bacillus thuringiensis, the former being used as a biological weapon and the latter as a pesticide. B. anthracis and B. thuringiensis are readily distinguished from B. cereus by the presence of plasmid-borne specific toxins (B. anthracis and B. thuringiensis) and capsule (B. anthracis). But phylogenetic studies based on the analysis of chromosomal genes bring controversial results, and it is unclear whether B. cereus, B. anthracis and B. thuringiensis are varieties of the same species2 or different species3,4. Here we report the sequencing and analysis of the type strain B. cereus ATCC 14579. The complete genome sequence of B. cereus ATCC 14579 together with the gapped genome of B. anthracis A20125 enables us to perform comparative analysis, and hence to identify the genes that are conserved between B. cereus and B. anthracis, and the genes that are unique for each species. We use the former to clarify the phylogeny of the cereus group, and the latter to determine plasmid-independent species-specific markers.read more
Citations
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High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries.
Chirag Jain,Luis M. Rodriguez-R,Adam M. Phillippy,Konstantinos T. Konstantinidis,Srinivas Aluru +4 more
TL;DR: FastANI is developed, a method to compute ANI using alignment-free approximate sequence mapping, and it is shown 95% ANI is an accurate threshold for demarcating prokaryotic species by analyzing about 90,000 proKaryotic genomes.
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Developments in the use of Bacillus species for industrial production
TL;DR: Developments from the understanding of the complex protein translocation machinery of Gram-positive bacteria should allow the resolution of current secretion challenges and make Bacillus species preeminent hosts for heterologous protein production.
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From soil to gut: Bacillus cereus and its food poisoning toxins
TL;DR: The toxins associated with foodborne diseases frequently caused by B. cereus are reviewed, and recent findings regarding the associated toxins are discussed, as well as the present knowledge on virulence regulation.
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Multilocus Sequence Typing of Bacteria
TL;DR: Multilocus sequence typing (MLST) was proposed in 1998 as a portable, universal, and definitive method for characterizing bacteria, using the human pathogen Neisseria meningitidis as an example.
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Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons.
TL;DR: An upper bound of roughly threefold overestimation of bacterial diversity resulting from cloning and sequencing of 16S rRNA genes from the environment is suggested, however, the inclusion of genomes with a single rrn operon may lower this correction factor to approximately 2.5.
References
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Journal ArticleDOI
Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—One Species on the Basis of Genetic Evidence
Erlendur Helgason,Ole Andreas Økstad,Dominique A. Caugant,Henning Johansen,Agnès Fouet,Michèle Mock,Ida K. Hegna,Anne-Brit Kolstø +7 more
TL;DR: It is shown by multilocus enzyme electrophoresis and by sequence analysis of nine chromosomal genes that B. anthracis should be considered a lineage of B. cereus and this determination is not only a formal matter of taxonomy but may also have consequences with respect to virulence and the potential of horizontal gene transfer within the B. Cereus group.
Journal ArticleDOI
Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with L-lysine
Andreas Peschel,Andreas Peschel,Ralph W. Jack,Michael Otto,L. Vincent Collins,Petra Staubitz,Graeme J. Nicholson,Hubert Kalbacher,Willem F. Nieuwenhuizen,Günther Jung,Andrej Tarkowski,Kok P. M. van Kessel,Jos A. G. van Strijp +12 more
TL;DR: A novel staphylococcal gene, mprF, which determines resistance to several host defense peptides such as defensins and protegrins constitutes a novel virulence factor, which may be of general relevance for bacterial pathogens and represents a new target for attacking multidrug resistant bacteria.
Journal ArticleDOI
Epidemiology and pathogenesis of Bacillus cereus infections.
TL;DR: Bacillus cereus is a causative agent in both gastrointestinal and in nongastrointestinal infections and a special surface structure of B. cereus cells, the S-layer, has a significant role in the adhesion to host cells, in phagocytosis and in increased radiation resistance.
Journal ArticleDOI
Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group.
Sabine Lechner,Ralf Mayr,Kevin P. Francis,B. M. Prüss,T. Kaplan,E. Wiessner-Gunkel,Gordon S. A. B. Stewart,Siegfried Scherer +7 more
TL;DR: The DNA sequences investigated exhibited a high degree of sequence similarity indicating a close relationship between the species of the B. cereus group, and strongly support a hitherto unrecognized fifth sub-group within the B.'s cereus species group comprising psychrotolerant, but not mesophilic, B. mycoides strains.
Journal ArticleDOI
“Black holes” and bacterial pathogenicity: A large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli
TL;DR: These results suggest that, as Shigella spp.
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