Q2. What have the authors contributed in "Bacterial cellulose production, properties and applications with different culture methods - a review" ?
The aims of this review are to provide an overview of the production of BC from different culture methods, to analyze the characteristics of particular BC productions, to indicate existing problems associated with different methods, and to choose suitable culture approaches for BC applications in different fields.
Q3. What have the authors stated for future works in "Bacterial cellulose production, properties and applications with different culture methods - a review" ?
Due to the outstanding advantages of this method, it is likely to be used widely in future research. Thus, 3D print technology can expand applications of BC-based materials in future work. It has been shown that modification of properties, AC CE PT ED M AN US CR IP T increasing yields, reducing production costs, and choosing appropriate industrial fabrication lines are the main future goals for all researchers. BC can be prepared with an oriented structure, a weave structure, a multiple fiber layer, or a gradient structure, and with desirable properties for particular applications such as blood scaffolds, tendon tissue, and transparent materials.
Q4. What is the effect of the increase in BC film thickness?
when nutrients are depleted by constant consumption, the increase in BC film thickness can affect the oxygen supply for strains, suppressing the formation of BC.
Q5. Why is cellulose not degradable in the physiological environment?
due to the lack of appropriate enzymes to break down the beta acetal linkages, cellulose is not degradable in the physiological environment.
Q6. What is the morphology of BC obtained from the trickling bed reactor?
BC obtained from the trickling bed reactor has excellent properties, such as high degree of -OH association, as well as high polymerization, purity, water holding capacity, porosity, and thermal stability.
Q7. What is the basic idea behind the design of an agitated/shaking culture?
The basic idea behind the design of an agitated/shaking culture was to increase or optimize the delivery of oxygen to the bacteria during culture.
Q8. What is the main influence of the continuous shear force during agitated culture?
Under rotationally agitated cultivation, the continuous shear force during agitated culture is the first influence that forms a spherical structure.
Q9. What is the way to prepare a sphere-like BC?
In the past, in order to prepare sphere-like BC, a hydrogel fiber cultivation method and electrospinning have been used to form spherical BC (Higashi and Mik, 2018).
Q10. What is the common method for the formation of BC?
The static culture method is a relatively simple technique with low shear force environment; therefore, it is the most frequently used technique for the formation of BC at lab scale.
Q11. What is the main reason for the use of a trickling bed?
a trickling bed reactor, a kind of vinegar manufacturing equipment, has also been used for BC production (Lu and Jiang, 2014).
Q12. What are some drawbacks of the agitated/shaking method?
In general, the instability of the bacterial strain, the non-Newtonian behavior during mixing of the BC, and the high shear force are some drawbacks of the agitated/shaking method.
Q13. What was the BC production rate in the airlift bioreactor?
With the use of oxygen-enriched air, the BC production rate increased to 0.093 g/l/h and the BC yield was enhanced from 11% in AC CE PT ED M ANUS CRIP Tair to 18%.
Q14. What is the effect of the agitated culture on BC productivity?
the appearance of a non-cellulose mutant usually results in a reduction of BC productivity in the agitated/shaking culture.
Q15. What is the drawback of the static culture method?
The delivery of oxygen is directly associated with the production of BC and is known as aACCEPT EDM ANUS CRIP Tmajor drawback of the static culture method.
Q16. What is the role of the rotation speed in the formation of sphere-like BC?
With a rotational speed less than 100 rpm, it is difficult to find any sphere-like BC particles; rather, irregular shapes of the synthesized BC have been observed.