Journal ArticleDOI
Barley embryo globulin 1 gene, Beg1: characterization of cDNA, chromosome mapping and regulation of expression.
TLDR
This paper reported identification of a 2189 bp cDNA clone from barley corresponding to a single-copy gene, Beg1 (Barley embryo globulin), on chromosome 4, which encodes a storage globulin.Abstract:
We report identification of a 2189 bp cDNA clone from barley corresponding to a single-copy gene, Beg1 (Barley embryo globulin), on chromosome 4, which encodes a storage globulin In barley, the major protein reserve in the aleurone layer belongs to the 7S globulin class of proteins found in many seeds Electrophoretically and antigenically similar proteins are present in the barley embryo Accumulation of Beg1 mRNA was noted beginning 15-20 days post-anthesis in both the aleurone layer and embryo of the developing barley grain but not in the starchy endosperm A high level of Beg1 mRNA is also present in the mature imbibed aleurones, which can be repressed by treatment with gibberellic acid This repressive effect of gibberellin on the levels of Beg1 mRNA is confirmed in the gibberellin response-constitutive mutant, slender, whose aleurone layers do not accumulate Beg1 mRNA even in the absence of applied gibberellic acid The deduced primary translation product of the Beg1 mRNA is a 637 amino acid (72 kDa) protein with homology to maize embryo globulin 1 (GLB1) and a partial sequence of a wheat 7S globulin The internal amino acid sequence of BEG1 closely matches the N-terminal sequence of isolated barley aleurone globulin Seven imperfect tandem repeats of 16 amino acids each are present near the N-terminus of BEG1, which conform to the consensus HGEGEREEEXGRGRGR, and contribute to the observed unusual amino acid composition of this protein A second, distinct barley globulin gene, Beg2, which is homologous to maize Glb2, was detected by Northern and Southern analysis Beg2 and Beg1 are regulated differently which may indicate variation in storage or utilization properties among the barley globulinsread more
Citations
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Journal ArticleDOI
Cereal seed storage proteins: structures, properties and role in grain utilization
Peter R. Shewry,Nigel G. Halford +1 more
TL;DR: The role of the gluten proteins of wheat in determining the quality of the grain for breadmaking and how their amount and composition can be manipulated leading to changes in dough mixing properties is discussed.
Journal ArticleDOI
Gibberellins : perception, transduction and responses
TL;DR: As the understanding of GA-regulated events in aleurone cells has advanced through molecular and cell biology approaches, other GA-responsive plant tissues have also proved to be tractible for studying GA-action.
Journal ArticleDOI
Developmental changes in the metabolic protein profiles of wheat endosperm.
William H. Vensel,Charlene K. Tanaka,Nick Cai,Joshua H. Wong,Bob B. Buchanan,William J. Hurkman +5 more
TL;DR: Analysis of protein number and spot volume revealed that carbohydrate metabolism, transcription/translation, and protein synthesis/assembly were the principal endosperm functions at 10 dpa followed by nitrogen metabolism, protein turnover, cytoskeleton, cell division, signal transduction, and lipid metabolism.
Journal ArticleDOI
AGL15, a MADS domain protein expressed in developing embryos.
TL;DR: It is suggested that AGL15 is likely to be an important component of the regulatory circuitry directing seed-specific processes in the developing embryo, and found in embryos of a variety of angiosperms, including plants as distantly related as maize.
Journal ArticleDOI
A family of antimicrobial peptides is produced by processing of a 7S globulin protein in Macadamia integrifolia kernels
TL;DR: The results suggest that some vicilins play a role in defence during seed germination, and the purification of the predicted family members MiAMP2b and 2d, both of which also exhibited antimicrobial activity in vitro, are suggested.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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