Patent
Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
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TLDR
In this paper, the gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxalkanoates using transgenic organisms, including both bacteria and plants.Abstract:
The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.read more
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Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.
Takakazu Kaneko,Shusei Sato,Hirokazu Kotani,Ayako Tanaka,Erika Asamizu,Yasukazu Nakamura,Nobuyuki Miyajima,Makoto Hirosawa,Masahiro Sugiura,Shigemi Sasamoto,Takaharu Kimura,Tsutomu Hosouchi,Ai Matsuno,Akiko Muraki,Naomi Nakazaki,Kaoru Naruo,Satomi Okumura,Sayaka Shimpo,Chie Takeuchi,Tsuyuko Wada,Akiko Watanabe,Manabu Yamada,Miho Yasuda,Satoshi Tabata +23 more
TL;DR: The sequence determination of the entire genome of the Synechocystis sp.