Biosynthesis of Natural Flavanones in Saccharomyces cerevisiae
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TLDR
A four-step flavanone biosynthetic pathway was constructed and introduced into Saccharomyces cerevisiae and produced the flavanones naringenin and pinocembrin 62 and 22 times more efficiently compared to previously reported recombinant prokaryotic strains.Abstract:
A four-step flavanone biosynthetic pathway was constructed and introduced into Saccharomyces cerevisiae. The recombinant yeast strain was fed with phenylpropanoid acids and produced the flavanones naringenin and pinocembrin 62 and 22 times more efficiently compared to previously reported recombinant prokaryotic strains. Microbial biosynthesis of the flavanone eriodictyol was also achieved.read more
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DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
Zengyi Shao,Hua Zhao,Huimin Zhao +2 more
TL;DR: A new method, DNA assembler, is reported, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae and represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.
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Current approaches toward production of secondary plant metabolites.
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TL;DR: The present review is aimed to cover phytotherapeutic application and recent advancement for the production of some important plant pharmaceuticals.
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BglBrick vectors and datasheets: A synthetic biology platform for gene expression
Taek Soon Lee,Taek Soon Lee,Rachel Krupa,Rachel Krupa,Fuzhong Zhang,Fuzhong Zhang,Meghdad Hajimorad,Meghdad Hajimorad,William J. Holtz,Nilu Prasad,Nilu Prasad,Sung Kuk Lee,Sung Kuk Lee,Sung Kuk Lee,Jay D. Keasling +14 more
TL;DR: The standardized collection of vectors presented here allows the user to rapidly construct and test the expression of genes with various combinations of promoter strength, inducible expression system, copy number, and antibiotic resistance.
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Genome-scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA.
TL;DR: Combined effect of the genetic interventions was found to be synergistic based on a developed analysis method that correlates genetic modification to cell phenotype, specifically the identified knockout targets and overexpression targets can cooperatively force carbon flux towards malonyl-CoA.
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Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
TL;DR: The metabolic engineering of central metabolic pathways described in the present work increased the production of pinocembrin, naringenin, and eriodictyol in 36 h up to 1,379%, 183%, and 373%, respectively, over production with the strains expressing only the flavonoid pathway.
References
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The Effects of Plant Flavonoids on Mammalian Cells:Implications for Inflammation, Heart Disease, and Cancer
TL;DR: Western medicine has not yet used flavonoids therapeutically, even though their safety record is exceptional, and suggestions are made where such possibilities may be worth pursuing.
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Guide to yeast genetics and molecular biology
Christine Guthrie,Gerald R. Fink +1 more
TL;DR: This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast.
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Polyphenols and disease risk in epidemiologic studies
TL;DR: A review of available epidemiologic data on the health effects of polyphenols, focusing on the flavonoid subclasses of flavonols, flavones, and catechins and on lignans, is presented in this paper.
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Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Guide To Yeast Genetics And Molecular Biology
TL;DR: The guide to yeast genetics and molecular biology is universally compatible with any devices to read and is available in the book collection an online access to it is set as public so you can get it instantly.