Journal ArticleDOI
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins
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TLDR
It is concluded that Rad54 acts to overcome kinetic impediments that would limit homologous DNA pairing between recombining chromosomes in vivo.Abstract:
The Saccharomyces cerevisiae RAD51 and RAD54 genes are both required for the occurrence of homologous recombination and for the repair of double-stranded DNA breaks1. Previous studies have indicated that Rad51 protein, together with the single-stranded DNA-binding factor replication protein A (RPA), can promote the formation of heteroduplex DNA2,3,4, which is a key intermediate in homologous recombination1. Here we report the purification of the Rad54 protein to near homogeneity and the biochemical testing of its molecular function. We find that Rad54 protein possesses a double-stranded DNA-dependent ATPase activity, and that it interacts with the Rad51 protein. Addition of Rad54 protein to reactions containing Rad51 strongly stimulates the rate of pairing between homologous single-stranded and double-stranded DNA molecules. We conclude that Rad54 acts to overcome kinetic impediments that would limit homologous DNA pairing between recombining chromosomes in vivo.read more
Citations
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Journal ArticleDOI
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more
TL;DR: This review encompasses different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
Journal ArticleDOI
Sensing and repairing DNA double-strand breaks.
TL;DR: An increased knowledge of DSB repair and of other DNA DSB responses may provide opportunities for developing more effective treatments for cancer, with particular emphasis on non-homologous end-joining and homologous recombination.
Journal ArticleDOI
Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair
TL;DR: An overview of recent biochemical and structural analyses of the Rad52 group proteins is provided and how this information can be incorporated into genetic studies of recombination is discussed.
Journal ArticleDOI
Molecular views of recombination proteins and their control
TL;DR: The efficient repair of double-strand breaks in DNA is critical for the maintenance of genome stability and cell survival, especially in replicating cells, in which it plays a major role in tumour avoidance.
Journal ArticleDOI
Homologous recombination in DNA repair and DNA damage tolerance
Xuan Li,Wolf Dietrich Heyer +1 more
TL;DR: Mechanistic aspects of HR relating to DSB and ICL repair as well as replication fork support related to DNA double-stranded breaks and interstrand crosslinks are reviewed.
References
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Journal ArticleDOI
Biochemistry of homologous recombination in Escherichia coli.
TL;DR: This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination.
Journal ArticleDOI
Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein
TL;DR: The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair and it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-Stranded form, indicating that RAD 51 can catalyze strand exchange.
Journal ArticleDOI
Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions.
TL;DR: This work has divided the SNF2 family into multiple subfamilies, each of which represents what it proposes to be a functionally and evolutionarily distinct group, and used the subfamily structure to predict the functions of some of the uncharacterized proteins in the SNf2 family.
Journal ArticleDOI
DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA
TL;DR: Results presented here indicate that only the RAD51-ssDNA nucleoprotein filament is functionally relevant and pairing and strand exchange initiate at the 5' end of the complementary strand in the linear duplex, a reaction polarity opposite to that of the bacterial prototype RecA.
Journal ArticleDOI
Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination.
Jeroen Essers,Rudolf W. Hendriks,Sigrid M. A. Swagemakers,Christine Troelstra,Jan de Wit,Dirk Bootsma,Jan H.J. Hoeijmakers,Roland Kanaar +7 more
TL;DR: It is shown that mRAD54-/- mice are viable and exhibit apparently normal V(D)J and immunoglobulin class-switch recombination, which implies that, besides DNA end-joining mediated by DNA-dependent protein kinase, homologous recombination contributes to the repair of DSBs in mammalian cells.
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Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase.
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
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