DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA
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TLDR
Results presented here indicate that only the RAD51-ssDNA nucleoprotein filament is functionally relevant and pairing and strand exchange initiate at the 5' end of the complementary strand in the linear duplex, a reaction polarity opposite to that of the bacterial prototype RecA.About:
This article is published in Cell.The article was published on 1995-08-11 and is currently open access. It has received 507 citations till now. The article focuses on the topics: Coding strand & Replication protein A.read more
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Journal ArticleDOI
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more
TL;DR: This review encompasses different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
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Mechanism of eukaryotic homologous recombination.
TL;DR: HR accessory factors that facilitate other stages of the Rad51- and Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.
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Association of BRCA1 with Rad51 in Mitotic and Meiotic Cells
Ralph Scully,Junjie Chen,Annemieke W. Plug,Yonghong Xiao,David R. Weaver,Jean Feunteun,Terry Ashley,David M. Livingston +7 more
TL;DR: Findings suggest a functional interaction between BRCA1 and Rad51 in the meiotic and mitotic cell cycles, which, in turn, suggests a role for BRC a1 in the control of recombination and of genome integrity.
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REPLICATION PROTEIN A: A Heterotrimeric, Single-Stranded DNA-Binding Protein Required for Eukaryotic DNA Metabolism
TL;DR: Replication protein A (RPA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism, including DNA replication, DNA repair, and recombination.
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Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2
Shyam K. Sharan,Masami Morimatsu,Masami Morimatsu,Urs Albrecht,Dae-Sik Lim,Eva Regel,Christopher Dinh,Arthur T. Sands,Gregor Eichele,Paul Hasty,Allan Bradley +10 more
TL;DR: Developmental arrest in Brca2-deficient embryos, their radiation sensitivity, and the association of Brca1 with Rad51 indicate that Brca 2 may be an essential cofactor in the Rad51-dependent DNA repair of double-strand breaks, thereby explaining the tumour-suppressor function of BrCA2.
References
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Journal ArticleDOI
Biochemistry of homologous recombination in Escherichia coli.
TL;DR: This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination.
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Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
TL;DR: It is suggested that the Rad51 protein, probably together with Rad52 protein, is involved in a step to convert DSBs to the next intermediate in recombination.
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DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
TL;DR: DMC1 phenotypes provide further evidence that recombination and SC formation are interrelated processes and are consistent with a requirement for DNA-DNA interactions during SC formation, and additional evidence suggests that arrest occurs at a meiosis-specific cell cycle "checkpoint" in response to a primary defect in prophase chromosome metabolism.
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Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein
TL;DR: The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair and it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-Stranded form, indicating that RAD 51 can catalyze strand exchange.
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A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae
TL;DR: In this article, the authors identify and analyze a meiotic reciprocal recombination hot spot in S. cerevisiae and find that double-strand breaks occur at two specific sites associated with the hot spot and that occurrence of these breaks depends upon meiotic recombination functions RAD50 and SPO11.