Journal ArticleDOI
Characterization of 2',7'-dichlorofluorescin fluorescence in dissociated mammalian brain neurons: estimation on intracellular content of hydrogen peroxide.
TLDR
The possibility that the DCF fluorescence may be useful in estimating the intracellular content of hydrogen peroxide of mammalian brain neurons is suggested.About:
This article is published in Brain Research.The article was published on 1994-01-28. It has received 181 citations till now. The article focuses on the topics: Reactive oxygen species & Catalase.read more
Citations
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Journal ArticleDOI
Oxidative stress in the pathogenesis of diabetic neuropathy.
TL;DR: Evidence is presented to support the idea that both chronic and acute hyperglycemia cause oxidative stress in the peripheral nervous system that can promote the development of diabetic neuropathy, and it is concluded that striving for superior antioxidative therapies remains essential for the prevention of neuropathy in diabetic patients.
Journal ArticleDOI
Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation
TL;DR: A critical role for mitochondria in the production of ROS in association with glutamate excitotoxicity is suggested, and the feasibility of measuring theProduction of ROS at the level of the single cell is demonstrated.
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Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species.
TL;DR: Results reveal that peroxynitrite, hypochlorous acid, and H2O2 plus peroxidase all oxidize DCDHF and DHR to varying degrees but that neither superoxide, H2 O2 alone, nor physiological levels of nitric oxide are capable of indicator oxidation.
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2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy
TL;DR: A comprehensive review of the basic characteristics of DCFH2 and the present views of the mechanisms of its fluorescence formation are discussed to underline its advantages and disadvantages in ROS detection as well as for the methodological considerations that arise during analysis.
Journal ArticleDOI
Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase:: Possible implications for oxidative stress measurements
TL;DR: DCF fluorescence cannot be used reliably to measure O2- in cells because O2*- itself is formed during the conversion of DCFH to DCF by peroxidases, and the use of this probe to measure H2O2 production in cells is problematic.
References
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Journal ArticleDOI
Hydroperoxide metabolism in mammalian organs.
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Oxygen-derived free radicals in postischemic tissue injury.
TL;DR: It is now clear that oxygen-derived free radicals play an important part in several models of experimentally induced reperfusion injury, and Dysfunction induced by free radicals may be a major component of ischemic diseases of the heart, bowel, liver, kidney, and brain.
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The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
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Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.