Journal ArticleDOI
Characterization of the subunits of Q-beta replicase.
Robert Kamen,Robert Kamen +1 more
TLDR
The RNA replicating enzyme induced by RNA phage Qβ consists of a single phage specific and three host specific polypeptide chains, and none are known subunits of E. coli DNA-dependent RNA polymerase.Abstract:
The RNA replicating enzyme induced by RNA phage Qβ consists of a single phage specific and three host specific polypeptide chains. At least one of the host subunits is shown to be essential for enzymatic activity, and none are known subunits of E. coli DNA-dependent RNA polymerase.read more
Citations
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Journal ArticleDOI
A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more
TL;DR: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein.
Journal ArticleDOI
Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene.
Walter Fiers,Roland Contreras,F Duerinck,Guy Haegeman,Dirk Iserentant,J. Merregaert,W. Min Jou,Francis Molemans,A Raeymaekers,A Van den Berghe,Guido Volckaert,M. Ysebaert +11 more
TL;DR: The complete, primary chemical structure of a viral genome has now been established and biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis.
Journal ArticleDOI
The concept of parasite-derived resistance—Deriving resistance genes from the parasite's own genome
John C. Sanford,S.A. Johnston +1 more
TL;DR: A broadly-applicable strategy for genetically engineering resistance to parasites involves deriving resistance genes from the genome of the parasite itself, and the QB bacteriophage is used to illustrate, specifically, how parasite-derived resistance might be engineered.
Book ChapterDOI
3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures
Klaus Weber,Mary Osborn +1 more
Journal ArticleDOI
Quaternary Structure of Proteins
TL;DR: The history and present situation of mushroom infestation in the United States, as well as some of the myths and legends surrounding the phenomenon, are described.
References
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Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
TL;DR: A method for the purification of Escherichia coli DNA-dependent RNA polymerase which is rapid, reproducible, high in yield, and able to handle preparations using from 1 g to 3 kg of cells is described.
Journal ArticleDOI
Separation and characterization of the subunits of ribonucleic acid polymerase.
TL;DR: It is concluded that phosphocellulose enzyme has the structure α2ββ' where the molecular weights of the chains α, β, and β' are 39,000 ± 5%, 155,000 +/- 10%, and 165,000± 10%, respectively.
Journal ArticleDOI
Analysis of C14-labeled proteins by disc electrophoresis
TL;DR: This report presents an alternative procedure involving autoradiography of dried longitudinal gel slices that is relatively uncomplicated and can be used to develop the entire pattern of radioactivity in a gel without significant sacrifice of resolution.