Journal ArticleDOI
Chemical Strategies for Generating Protein Biochips
TLDR
Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function.Abstract:
Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.read more
Citations
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Journal ArticleDOI
Point of care diagnostics: Status and future
TL;DR: This chapter discusses the development of personalized medicine and home testing in the developing world, and some of the strategies used to achieve this goal have not yet been developed.
Journal ArticleDOI
Protein interactions with polymer coatings and biomaterials.
Qiang Wei,Tobias Becherer,Stefano Angioletti-Uberti,Joachim Dzubiella,Christian Wischke,Axel T. Neffe,Andreas Lendlein,Matthias Ballauff,Rainer Haag +8 more
TL;DR: Current analytical methods to test mechanistic hypotheses and theories of protein-surface interactions will be discussed and special focus will be given to state-of-the-art bioinert and biospecific coatings and their applications in biomedicine.
Journal ArticleDOI
Force probing surfaces of living cells to molecular resolution
TL;DR: How atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution is reviewed to provide unique insight into how cells structurally and functionally modulate the molecules of their surfaces to interact with the cellular environment.
Journal ArticleDOI
Single molecule sensing with solid-state nanopores: novel materials, methods, and applications
Benjamin N. Miles,Aleksandar P. Ivanov,Kerry A. Wilson,Fatma Dogan,Deanpen Japrung,Deanpen Japrung,Joshua B. Edel +6 more
TL;DR: This tutorial review will introduce and explore the fundamental aspects of nanopore (bio)sensing, fabrication, modification, and the emerging technologies and applications that both intrigue and inspire those working in and around the field.
Journal ArticleDOI
Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack
TL;DR: Current methods for target identification of small molecules are summarized, primarily for a chemistry audience but also the biological community, for example, the chemist or biologist attempting to identify the target of a given bioactive compound.
References
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