Cholesterol ester formation in cultured human fibroblasts. Stimulation by oxygenated sterols.
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TLDR
The data suggest that the processes of cholesterol ester formation and cholesterol synthesis in human fibroblasts are regulated in a reciprocal manner by coordinate changes in the activities of these two membrane-bound enzymes.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-05-25 and is currently open access. It has received 348 citations till now. The article focuses on the topics: Reverse cholesterol transport & Acyltransferase.read more
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Book ChapterDOI
Receptor-mediated endocytosis of low-density lipoprotein in cultured cells
TL;DR: Study of the cell surface binding, internalization, and metabolism of low-density lipoprotein (LDL) in cultured cells have provided useful information regarding the general aspects of receptor-mediated endocytosis and three classes of mutant alleles at the LDL receptor locus have been deduced.
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Protein Sensors for Membrane Sterols
TL;DR: Recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake are discussed.
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Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth
TL;DR: The availability of compactin (ML-236B), a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl Coenzyme A reductase, has permitted the demonstration of a hitherto unsuspected aspect of mevalonate metabolism and isoprenoid synthesis in cultured mammalian cells.
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The LDL Receptor
TL;DR: The history of the LDL receptor is recounted by its codiscoverers to explain a genetic cause of heart attacks and introduce three general concepts to cell biology: receptor-mediated endocytosis, receptor recycling, and feedback regulation of receptors.
Journal ArticleDOI
Oxysterols: modulators of cholesterol metabolism and other processes.
TL;DR: This review comprises a detailed and critical assessment of current knowledge regarding the formation, occurrence, metabolism, regulatory properties, and other activities of oxysterols in mammalian systems.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Binding and Degradation of Low Density Lipoproteins by Cultured Human Fibroblasts COMPARISON OF CELLS FROM A NORMAL SUBJECT AND FROM A PATIENT WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA
TL;DR: It is raised that a prerequisite for the regulation of cholestero-genesis in cultured fibroblasts is the initial binding of low density lipoproteins to the high affinity surface receptor sites and that a defect in this process represents the primary genetic abnormality in the disorder familial hypercholesterolemia.
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Regulation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Activity in Cultured Human Fibroblasts COMPARISON OF CELLS FROM A NORMAL SUBJECT AND FROM A PATIENT WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA
TL;DR: Cultured cells from a homozygote with the disorder familial hypercholesterolemia, which showed a nearly complete failure of suppression of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity by low density or very low density lipoproteins, synthesized enzyme molecules at a rate nearly 60 times greater than that of normal cells.
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Familial hypercholesterolemia: Defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3-methylglutaryl coenzyme a reductase activity
TL;DR: The demonstration of a defect in binding of low-density lipoproteins to cells from subjects with the homozygous form of familial hypercholesterolemia appears to explain the previously reported failure of lipoproteinins to suppress the synthesis of this enzyme and hence may account for the overproduction of cholesterol that occurs in these cultured cells.
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Suppression of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Activity and Inhibition of Growth of Human Fibroblasts by 7-Ketocholesterol
TL;DR: It is concluded that 7-ketocholesterol may provide a powerful tool for delineation of the mechanism by which cholesterol and other steroids promote the growth of human cells in culture.