Journal ArticleDOI
Chromosome mapping and expression of a putative testis-determining gene in mouse.
TLDR
Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes, and mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.Abstract:
Isolation and mapping of a mouse complementary DNA sequence (mouse Y-finger) encoding a multiple, potential zinc-binding, finger protein homologous to the candidate human testis-determining factor gene is reported. Four similar sequences were identified in Hind III-digested mouse genomic DNA. Two (7.2 and 2.0 kb) were mapped to the Y chromosome. Only the 2.0-kb fragment, however, was correlated with testis determination. Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes. A 3.6-kb fragment was mapped to the X chromosome between the T16H and T6R1 translocation breakpoints, and a fourth (6.0 kb) was mapped to chromosome 10. Hence, mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.read more
Citations
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Journal ArticleDOI
Male development of chromosomally female mice transgenic for Sry
TL;DR: It is shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos.
Journal ArticleDOI
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
John Gubbay,Jérôme Collignon,Peter Koopman,Blanche Capel,Androulla Economou,Andrea Münsterberg,Nigel Vivian,Peter N. Goodfellow,Robin Lovell-Badge +8 more
TL;DR: A gene mapping to the sex-determining region of the mouse Y chromosome is deleted in a line of XY female mice mutant for Tdy, and is expressed at a stage during male gonadal development consistent with its having a role in testis determination.
Journal ArticleDOI
The origin and efficient derivation of embryonic stem cells in the mouse
F. A. Brook,Richard L. Gardner +1 more
TL;DR: By explanting tissues isolated microsurgically from implanting strain 129 mouse blastocysts individually on STO feeder cells, it is established that embryonic stem (ES) cells originate from the epiblast (primitive ectoderm).
Journal ArticleDOI
Evolution of the Zfx and Zfy genes: rates and interdependence between the genes.
Pekka Pamilo,Néstor O. Bianchi +1 more
TL;DR: A phylogenetic analysis of sex-chromosomal zinc-finger genes (Zfx and Zfy) indicates that the genes have not evolved completely independently since their initial separation.
Journal ArticleDOI
Mouse embryonic germ (EG) cell lines : transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines
TL;DR: The derivation of multiple embryonic germ cell lines from 8.5 days post coitum embryos of C57BL/6 inbred mice are reported, showing that pluripotent cell lines capable of forming teratocarcinomas and coat color chimeras can be established from primordial germ cells of 8.0 days p.c.
References
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
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TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
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Repetitive zinc‐binding domains in the protein transcription factor IIIA from Xenopus oocytes.
TL;DR: Analysis of the amino acid sequence reveals nine tandem similar units, each consisting of approximately 30 residues and containing two invariant pairs of cysteines and histidines, the most common ligands for zinc in the 7S particle of Xenopus laevis oocytes, which suggests that the protein contains repetitive zinc‐binding domains.
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Wound Macrophages Express TGF-α and Other Growth Factors in Vivo: Analysis by mRNA Phenotyping
TL;DR: Macrophages isolated from a wound site, and not exposed to cell culture conditions, expressed messenger RNA transcripts for TGF-alpha, T GF-beta, platelet-derived growth factor A-chain, and insulin-like growth factor-1 by a novel method for RNA analysis.